In this paper we present the numerical simulation-based design of a new microfluidic device concept for electrophoretic mobility and (relative) concentration measurements of dilute mixtures. The device enables stationary focusing points for each species, where the locally applied pressure driven flow (PDF) counter balances the species’ electrokinetic velocity. The axial location of the focusing point, along with the PDF flowrate and applied electric field reveals the electrokinetic mobility of each species. Simultaneous measurement of the electroosmotic mobility of an electrically neutral specie can be utilized to calculate the electrophoretic mobility of charged species. The proposed device utilizes constant sample feeding, and results in time-steady measurements. Hence, the results are independent of the initial sample distribution and flow dynamics. In addition, the results are insensitive to the species diffusion for large Peclet number flows (Pe > 400), enabling relative concentration measurement of each specie in the dilute mixture.