In the four-year experiment the impact of four different barley varieties and selected two-and three-component mixtures were tested. The studies were carried out at two sites: Experimental Station for Variety Testing Słupia Wlk. (Wielkopolska region) and Plant Breeding Station Bąków (Opole District) during four growing seasons (2001/2002–2004/2005). The aim of the studies was to evaluate the yield through growing barley variety mixtures in combination with reduced use of fungicide and application dose rates. Positive effects (1–15% yield increase in mixtures compared to pure stands) were observed. On the base of obtained results it can be stated that winter barley variety mixtures can constitute an alternative way of growing winter barley, especially at low-input and ecological agriculture.