Culture supernatant containing laccase produced by Cerrena unicolor strain was used to examine laccase partitioning between phases in an aqueous two-phase system. The investigated system consisted of polyethylene glycol 3000 and sodium phosphate buffer adjusted to pH = 7. Influence of several parameters on partitioning was measured, including phase forming components’ concentrations, tie line lengths, phase volume ratio, supernatant dilution, process temperature and halogen salt supplementation. Partitioning coefficients up to 78 in the bottom phase were achieved with yields of over 90%. Tie line length and phase volume ratio had significant effect on enzyme partitioning.

In this paper, a study of the lightning phenomenon and its harmful effect on Aqaba Thermal Power Station (ATPS), located in the south-western border of Jordan, is presented using the Electromagnetic Transients Program – Alternative Transients Program (EMTP- ATP). This study has been arisen due to an installation need of appropriate lightning arresters (LAs) for the 15/410 kV step-up transformers of the ATPS to eliminate the destructive effect of lightning. The simulation is carried out for two cases, once without using LAs and once more with using them. Two scenarios are applied for each of these cases, once when lightning strikes the primary side of the transformer and once more when it strikes the secondary side. The results obtained by the simulation indicate the necessity of LAs installation. This study, with using the EMTP-ATP program, is done for the first time with additional details that help researchers, designers, and engineers to get a broad overview of the ATPS in order to protect it against lightning.