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Abstract

Agriculture is a signifi cant source of gaseous pollutants such as ammonia, methane, nitrous oxide and volatile organic compounds. Ammonia is particularly important due to the high emission and local, as well as global impact on the environment. The release of NH3 is one of the main ways of nitrogen emission to the atmosphere and it contributes to its subsequent deposition. The aim of the study was to analyze ammonia emissions from animal production in Poland in 2005–2017, its regional diversity and possibilities of its reduction in agriculture. The ammonia emission was calculated for the animal production groups according to the NFR classifi cation. The values of ammonia emission were calculated based on ammonia emission factors used by KOBIZE, in accordance with the EMEP/EEA methods. In 2017, the NH3 emission from Polish agriculture amounted 288 Gg and it accounted for 96% of the emission in 2005. Ammonia emission from livestock production, in 2005–2017, on average accounted for 79.8% of agricultural emissions. The largest share had the cattle (51%) and swine (30%) production. The NH3 emissions differed strongly between provinces. The emission density (kg NH3·km-2·year-1) in provinces with intensive livestock production was about 5.5 times higher than in regions, where livestock production was the lowest. The mitigation strategies should be implemented primarily in provinces where reduction potential is the largest. The assessment of the reduction potential should take into account the NH3 emission per 1 km2 and the low

NH3 emission technologies, which are already applied in the regions.

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Authors and Affiliations

Paulina Mielcarek-Bocheńska
Wojciech Rzeźnik
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Abstract

Livestock production is the basis of global food production and it is a serious threat to the environment. Significant environmental pollutants are odors and ammonia (NH3) emitted from livestock buildings. The aim of the study was to determine the concentration and emission factors of ammonia and odors, in the summer season, from a deep-litter fattening house. The research was carried out during summer in a mechanically ventilated fattening piggery located in the Greater Poland Voivodeship. Ammonia concentrations were measured using photoacoustic spectrometer Multi Gas Monitor Innova 1312, and odor concentrations were determined by dynamic olfactometry according to EN 13725:2003 using a TO 8 olfactometer. The NH3 emission factors from the studied piggery, in summer, ranged from 8.53 to 21.71 g·day-1·pig-1, (mean value 12.54±4.89 g·day-1·pig-1). Factors related to kg of body mass were from 0.11 to 0.23 g·day-1·kg b.m.-1 (mean value 0.17±0.06 g·day-1·kg b.m.-1). Odor concentrations in the studied piggery were from 755 to 11775 ouE·m-3 and they were diversified (coefficient of variation 43.8%). The mean value of the momentary odor emission factors was 179.5±78.7 ouE·s-1·pig-1. Factor related to kg of body mass was 2.27±1.71 ouE·s-1·kg b.m.-1. In Poland and many other countries, the litter systems of pigs housing are still very popular. Therefore, there is a need to monitor the pollutant emissions from such buildings to identify the factors influencing the amount of this emission. Another important issue is to verify whether the reduction techniques, giving a measurable effect in laboratory research, bring the same reduction effect in production
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Bibliography

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Authors and Affiliations

Paulina Mielcarek-Bocheńska
1
ORCID: ORCID
Wojciech Rzeźnik
2

  1. Institute of Technology and Life Sciences-National Research Institute, Poland
  2. Poznan University of Technology, Poland
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Abstract

Various types of events and emergency situations have a significant impact on the safety of people and the environment. This especially refers to the incidents involving the emission of pollutants, such as ammonia, into the atmosphere. The article presents the concept of combining unmanned aerial vehicles with contamination plume modelling. Such a solution allows for mapping negative effects of ammonia release caused by the damage to a tank (with set parameters) during its transport as well as by the point leakage (such as unsealing in the installation). Simulation based on the ALOHA model makes it possible to indicate the direction of pollution spread and constitutes the basis for taking action. And, the use of a drone allows to control contamination in real time and verify the probability of a threat occurring in a given area.
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Authors and Affiliations

Anna Rabajczyk
1
Jacek Zboina
1
Maria Zielecka
1
Radosław Fellner
2
Piotr Kaczmarzyk
1
Dariusz Pietrzela
1
Grzegorz Zawistowski
1

  1. Scientific and Research Centre for Fire Protection, National Research Institute, Nadwiślańska 213, 05-420 Józefów, Poland
  2. Fire University of Warsaw, Słowackiego 52/54, 01-629 Warsaw, Poland
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Abstract

Phylloplane microbes have been studied as strategic tools in management against plant pathogens. Non-pathogenic bacteria and fungi have been applied as crop protectants against various plant diseases. The present study aimed at evaluating the potentiality of Aspergillus niger spores in altering the activity of four key enzymes related to defense in tomato. The experiment was designed such that two groups of 50 tomato plants were considered: group 1 – sprayed with autoclaved distilled water (control) and group 2 – sprayed with A. niger spores. Spraying was carried out under aseptic conditions. The experimental parameters included analysis of the activity of peroxidase (POX), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and tyrosine ammonia lyase (TAL) as well as expression of POX and PPO isoforms. The results demonstrated an inductive effect of A. niger on the activity of POX, PPO, PAL and TAL. Enhanced expression of POX and PPO isoforms was also observed. The results indicated that A. niger can be considered probiotic for the management of tomato against its phytopathogens.

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Authors and Affiliations

Susmita Goswami
ORCID: ORCID
Prabir Kumar Paul
Prem Datt Sharma
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Abstract

In the study an accurate energy and economic analysis of the carbon capture installation was carried out. Chemical absorption with the use of monoethanolamine (MEA) and ammonia was adopted as the technology of carbon dioxide (CO2) capture from flue gases. The energy analysis was performed using a commercial software package to analyze the chemical processes. In the case of MEA, the demand for regeneration heat was about 3.5 MJ/kg of CO2, whereas for ammonia it totalled 2 MJ/kg CO2. The economic analysis was based on the net present value (NPV) method. The limit price for CO2emissions allowances at which the investment project becomes profitable (NPV = 0) was more than 160 PLN/Mg for MEA and less than 150 PLN/Mg for ammonia. A sensitivity analysis was also carried out to determine the limit price of CO2emissions allowances depending on electricity generation costs at different values of investment expenditures.
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Authors and Affiliations

Krzysztof Bochon
Tadeusz Chmielniak
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Abstract

A novel absorbing pervaporation hybrid technique has been evaluated experimentally for the recovery of ammonia from the gas mixture in a recycle loop of synthesis plants. This process of hybridization brings together the combination of energy-efficient membrane gas separation based on poly(dimethylsiloxane) poly(diphenylsilsesquioxane) with a high selective sorption technique where a water solution with polyethylene glycol 400 (PEG-400) was used as the liquid absorbent. Process efficiency was studied using the pure and mixed gases. The influence of PEG-400 content in aqueous solutions on process selectivity and separation efficiency was studied. The ammonia recovery efficiency evaluation of an absorbing pervaporation technique was performed and compared with the conventional membrane gas separation. It was shown that the absorbing pervaporation technique outperforms the conventional membrane method in the whole range of productivity, producing the ammonia with a purity of 99.93 vol.% using the PEG 80 wt.% solution. The proposed method may be considered as an attractive solution in the optimization of the Haber process.

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Authors and Affiliations

Artem A. Atlaskin
Anton N. Petukhov
Nail R. Yanbikov
Maria E. Salnikova
Maria S. Sergeeva
Vladimir M. Vorotyntsev
Ilya V. Vorotyntsev
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Abstract

Most satellites stationed in space use catalytic propulsion systems for attitude control and orbit adjustment. Hydrazine is consumed extensively as liquid monopropellant, in the thrusters. Catalytic reactor is the most important section in the catalytic thruster. Ammonia and nitrogen gases are produced as a result of complete catalytic decomposition of hydrazine in the reactor, causing an increase in temperature and a rise in specific impulse. Ammonia is subsequently decomposed, leading to nitrogen and hydrogen gases. Decomposition of ammonia leads to a decrease in temperature, molecular weight and specific impulse. The latter phenomenon is unavoidable. The effect of ammonia decomposition on the reactor temperature, molecular weight of gaseous products and conclusively on specific impulse was studied in this article. At adiabatic state, thermodynamic analysis revealed that the maximum and minimum temperatures were 1655 K and 773 K, respectively. The highest molecular weight was obtained at ammonia conversion of zero and the lowest when ammonia conversion was 100%. The maximum specific impulse (305.4 S) was obtained at ammonia conversion of zero and completely conversion of ammonia, the minimum specific impulse (about 213.7 s) was obtained. For specific impulse, the result of thermodynamic calculation in this work was validated by the empirical results.

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Authors and Affiliations

Shahram Pakdehi
Fatemeh Shirvani
Reihaneh Zolfaghari
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Abstract

The Directive on National Emission Ceilings specifies the reduction of ammonia (NH 3) emissions among other air pollutants, which is most significant for the agricultural sector. The ammonia emission limit set for Hungary was a 10% reduction by 2020, while the target of 32% should be reached by 2030 compared to the 2005 reference year. The paper presents the results of a survey on pig production technology in Hungary from 97 domestic farms. The study aims to know the level of implementation of reduction techniques in livestock production and manure management and highlights the need for further improvements in this production sector. The research found that the application of ammonia reduction techniques was not considered widespread, either in livestock buildings or in manure storage (treatment) and during field application. For almost all (more than 90%) pig production groups, the housing systems were the reference without additional emission reduction. For manure storage, farms have insulated storage under the current regulation, however, significantly more emission reduction technologies were in the variant without cover or crust. Slurry spreading was mainly used with manure application techniques, but more emission-friendly injection and band spreading were also emerging. Besides the expected immediate incorporation, a high proportion of manure was applied between 12 and 24 hours or even after 24 hours. In the studied elements of manure management, significant improvements are needed in applying techniques to reduce ammonia emissions. Effective results can be achieved even by shortening the time between manure application and incorporation with efficient work organization.
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Bibliography

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Authors and Affiliations

Tibor Vojtela
1
Marianna Magyar
3
Sándor Koós
3
Nóra Péterfalvi
2
László Fenyvesi
2
Béla Pirkó
3

  1. Hungarian University of Agriculture and Life Sciences, University Laboratory Center, Hungary
  2. Hungarian University of Agriculture and Life Sciences, Institute of Technology, Hungary
  3. Centre for Agricultural Research, Institute of Soil Sciences, Hungary
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Abstract

Barley phylloplane is seriously colonized by Drechslera graminea, the causal agent of leaf

stripe disease in the hos. The present study involved the elucidation of alterations induced

in the protein content of the host due to Drechslera infection. Naturally growing barley

plants were obtained from fields and Drechslera graminea was isolated and identified from

diseased plants’ leaves. After identification and preparation of the pure culture, the pathogen

was inoculated on plants grown under aseptic and controlled laboratory conditions.

Changes in the total soluble cytoplasmic proteins and defense enzymes of the host such

as polyphenol oxidase (PPO), peroxidase (POX), phenylalanine lyase (PAL) and tyrosine

ammonia lyase (TAL) were observed up to 5 h after inoculation. The results demonstrated

a significant effect of the pathogen on the cytoplasmic protein expression of the host as well

as in its defense system.

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Authors and Affiliations

Navodit Goel
Gaurav Jaiswal
Abhinav Kr Srivastava
Prabir Kumar Paul
Anukrati Goel
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Abstract

This study examined the effects of UV-B radiation and allelochemical stress induced by ferulic acid (FA) on the activity of phenylalanine ammonia lyase (PAL; EC 4.3.1.5) at metabolic and molecular levels in two cucumber genotypes differing in tolerance to cold and disease, in order to determine any interaction between stress effects and genotype response. Stresses were applied simultaneously, sequentially, and singly. In both genotypes, several days of UV radiation retarded growth up to 36%. The effect of FA was not significant. The response to a particular stress, including the effect on PAL activation, was enhanced by simultaneous application of the two stresses. PAL transcription was not correlated with the increase of PAL activity. Exposure to UV-B, FA, and combined UV-B and FA was detrimental to both genotypes but to different extents. The response was not correlated with the genotype of cold and disease sensitivity. PAL activity and its transcription seem to be involved in UV and allelochemical stress, but not related to the plants' tolerance of these stresses.

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Authors and Affiliations

Anna Jóźwiak-Żurek
Monika Kozłowska
Katarzyna Nuc
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Abstract

Ammonia solutions are considered to be effective solvents for carbon dioxide absorption. Despite numerous advantages of these solvents, their high volatility is a significant technical and economic problem. Therefore, in this work, silica particles were used as additives to improve CO 2 absorption and inhibit NH 3 desorption. SiO 2 microparticles and colloidal SiO 2 particles in the concentration range of 0-0.15 wt.% were used in this study. The most favorable mass transport for CO2 absorption was at the concentration of colloidal particles of 0.05 wt.%. Under these conditions, the enhancement in the number of moles of absorbed CO 2 was above 30%. However, in solvents containing 0.01 wt.% SiO2 microparticles, the increase in CO 2 absorption was about 20%. At the same time, the addition of SiO2 particles significantly reduced the escape of ammonia from the solution. The best improvement was obtained when colloidal SiO 2 particles were added, and then NH 3 escape was decreased by about 60%. This unfavorable phenomenon was also inhibited in ammonia solutions containing SiO2 microparticles at a concentration of 0.01 wt.%.
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Authors and Affiliations

Donata Konopacka-Łyskawa
1
ORCID: ORCID
Temesgen Abeto Amibo
1 2
ORCID: ORCID
Dominik Dobrzyniewski
1
ORCID: ORCID
Marcin Łapiński
3
ORCID: ORCID

  1. Gdańsk University of Technology, Faculty of Chemistry, Department of Process Engineeringand Chemical Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
  2. School of Chemical Engineering, Jimma Institute of Technology, Jimma University, Jimma,P.O. Box-378, Ethiopia
  3. Gdańsk University of Technology, Faculty of Applied Physics and Mathematics, Institute ofNanotechnology and Materials Engineering, Narutowicza 11/12, 80-233 Gdańsk, Poland
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Abstract

Ammonia-oxidizing bacteria communities were evaluated in a completely mixed, laboratory scale membrane reactor (MBR) working under anoxic conditions for 5 months. The microorganisms in activated sludge were fed a synthetic medium containing 66-150 mg NH4 +-N/l. The age of the activated sludge in MBR was 50 days and the hydraulic retention time (HRT) was 3.3 days. The estimation of the diversity and complexity of the AOB community together with the identification of the dominant bacteria in the activated sludge under anoxic conditions were performed using denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. Molecular analysis of the microbial community carried out with two microbial molecular markers, 16S rRNA gene and amoA gene, suggested that nitrification was led by a Nitrosomonas-like species. In the biocenosis of the investigated bioreactor, oxygen was the crucial selective parameter. The results obtained in this work showed that amoA gene research is more suitable to study the stability and effectiveness of ammonia oxidation. This information emphasizes the necessity of the usage of molecular markers based on functional genes instead of ribosomal ones in order to present the actual state of the process performed in bioreactors. It was also stated that Nitrosomonas -like bacteria are able to perform nitritation even in anoxic environment, that is probably the reason why these bacteria are the most common AOB in different bioreactors.

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Authors and Affiliations

Aleksandra Ziembińska
Sławomir Ciesielski
Anna Raszka
Korneliusz Miksch
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Abstract

Root associated bacteria were isolated from Suaeda nudiflora and two isolates were selected for this study: rhizospheric Bacillus megaterium and endophytic Pseudomonas aeruginosa. These isolates were inoculated into maize variety Narmada Moti during its germination. TTC (2, 3, 5-triphenyl tetrazolium chloride) staining was used to confirm the association of the isolates with the maize root. The effects of these root associated bacteria were tested alone and in combinations for cell wall reinforcement and the induction of defense enzymes such as phenylalanine ammonia lyase (PAL) and β-1,3-glucanase in the presence of fungal pathogen Aspergillus niger in maize. The results indicated that the rhizospheric bacteria had a greater fight response to fungal infection than the endophhytic bacteria due to cell wall lignification as well as the rapid induction of higher concentrations of defense related enzymes.

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Authors and Affiliations

Yachana Jha
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Abstract

This study presents the results of research on the effect of long-term use of phosphorus fertilizers on permanent sugar beet crops for more than 50 years and on the transformation of phosphate forms on light chestnut soil and its yield. Our work aims to establish the main factors of quantitative and qualitative changes in various phosphates in light chestnut soil. Despite the large amount of practical material, the influence duration of phosphorus fertilizer application has not been sufficiently studied on the irrigated soils of Kazakhstan. It should be noted that the current study was carried out in long-term stationary experimental sites for the production of sugar beet with permanent sowing. The introduction of phosphate fertilizers primarily on the permanent crops of sugar beets in the same norms contributes to a more significant increase in gross phosphorus reserves. The soil content of gross phosphorus for 58 years on the control and nitrogen-potassium variants show practically no changes. Furthermore, when phosphorus fertilizers are applied on the variant with the annual application of a single norm of phosphorus and its amount for 58 years (4400 kg∙ha–1 of application doses) its content increased by 2660 mg∙kg–1, and with the introduction of its one and a half norms (6600 kg of application doses) by 2860 mg∙kg–1 of soil.
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Authors and Affiliations

Balnur Alimbekova
1
ORCID: ORCID
Rakhimzhan Yeleshev
1 2
ORCID: ORCID
Zhenisgul Bakenova
ORCID: ORCID
Aigerim Shibikeyeva
ORCID: ORCID
Marzhan Balkozha
ORCID: ORCID

  1. Kazakh National Agrarian University, Faculty of Agronomy, Abay avenue 8, Almaty 050010, Kazakhstan
  2. National Academy of Sciences of the Republic of Kazakhstan, Almaty, Kazakhstan

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