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Abstract

The significance of American expos in the history of world’s fairs, as well as in the history of planning, urban design, architecture and landscape architecture, is extraordinary. In the article, which constitutes a supplement to the first part of a report on the carrying out of the research project entitled “Urban and architectural heritage, as well as the impact of American world expos on city planning and development”, a retrospective outlook on American fairs has been presented on the examples of Philadelphia, Buffalo, Saint Louis and Cleveland, with a particular focus on traces of expos left in the cityscape and their long-term heritage, which affects the development of host-cities.
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Authors and Affiliations

Izabela Sykta
1
ORCID: ORCID

  1. Cracow University of Technology, Faculty of Architecture, Institute of Landscape Architecture
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Abstract

Twenty apparently healthy buffaloes were withdrawn of feed and water for 48 hours. Buffaloes were administered with fluids and were subjected to endoscopy every 12 hours. Olympus™ [GIF V70] flexible video endoscope was passed through the ventral nasal meatus, the pharynx, oesophagus and then into the reticulo-rumen in physically restrained buffaloes. The entire reticulum and part of the rumen could be visualized, when the animals were off feed and water for at least 48 hours and evacuations of rumen contents were done even after 48 hours of starvation to visualize the rumen in six buffaloes. The reticulum appeared light brown to pink coloured with honeycomb shape and the rumen appeared smooth, shiny pink, with numerous papillae throughout its surface. The procedure was well tolerated by all the buffaloes and satisfactory reticular and ruminal images could be obtained including biopsy.
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Bibliography

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Authors and Affiliations

K. Sasikala
1
G. Vijayakumar
2
S. Sivaraman
3
G.A. Balasubramaniam
4

  1. Department of Veterinary Clinical Medicine, Veterinary College and Research Institute, Namakkal, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu 637 002, India
  2. Veterinary University Peripheral Hospital, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu 637 002, India
  3. Department of Veterinary Clinics, Veterinary College and Research Institute, Namakkal, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu 637 002, India
  4. Department of Veterinary Pathology, Veterinary College and Research Institute, Namakkal, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu 637 002, India
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Abstract

The aim of this study was to analyse and identify specific buffalo seminal plasma proteins (SPPs) responsible for sperm cryotolerance during low temperature storage. Computer Assisted Sperm Analysis (CASA) of the motility and viability of buffalo spermatozoa was performed before freezing and after thawing. Two sample groups were formed – ejaculates with high cryotol- erance (group A) and low cryotolerance (group B). CASA demonstrated that the initial progres- sive motility after thawing of the spermatozoa in group A is significantly higher than in group B (p<0.001). Group B showed a significant increase in the percentage of static and non-progressive spermatozoa at 240 min, when compared to group A (p<0.05). SPPs, proteins in the cryoprotec- tive medium (PM) and proteins in the mixture of PM and SP were separated by High Perfor- mance Liquid Chromatography (HPLC). Comparative analysis of the chromatographic profiles was performed to identify specific proteins related to sperm cryotolerance. SPPs profiles showed 5 distinct protein peaks in both groups, ranging from 500 kDa to 50 Da. Chromatograms of group A and group B showed quantitative and qualitative differences in protein content. Chromato- grams of proteins in PM showed 11 well-expressed peaks. HPLC analysis of the mixtures of SPPs from the two groups and PM visualized the formation of a new bio-complex structure expressed by a protein peak specific for group A (7.674 min, AU 1.50). This protein peak can be referred as a phenotypic trait for buffalo ejaculates with high sperm cryotolerance.

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Authors and Affiliations

M.G. Ivanova
D.G. Gradinarska
T.S. Tsvetkov
I.V. Kirilova
B.A. Georgiev
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Abstract

Mastitis is one of the most crucial diseases of dairy animals. Especially subclinical mastitis (SCM) has negative impacts on of dairy economy in term of reducing milk quality and quantity also premature culling and cost of therapy. Staphylococci are important etiological agents in SCM. The aim of the study was to investigate the biofilm production and antibiotic resistance profiles of Staphylococcus spp. other than S. aureus isolated from milks of Anatolian water buffalo with subclinical mastitis. Twenty-two coagulase negative staphylococci (CNS) identified phenotypically were also identified with PCR as Staphylococcus spp. other than S. aureus. Biofilm productions were investigated both by Congo Red Agar Method and PCR. The antibiotic resistance profiles of the isolates were determined by Disc Diffusion Method and they were antibiotyped. Only three (13.6%) isolates were biofilm positive both phenotypically and genotypically. All isolates except for two were resistant against at least two antibiotics. Multidrug-resistance among the isolates was low (13.6%). Antibiotyping results showed that the similarities among the strains were between 30-100%. Genotyping of the strains revealed that a genetic heterogeneity was found among CNS isolates and their similarities were between 43% and 93%. In conclusion, CNS isolates identified as subclinical mastitis agents in buffaloes showed a high antibiotic resistance profile especially against oxacillin and vancomycin. Further studies should be conducted to investigate new mechanisms and/or genes responsible for antibiotic resistance in buffaloes.
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Authors and Affiliations

H. Gurler
1
A. Findik
2
M.G. Sezener
2

  1. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey
  2. Department of Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey

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