The purpose of the present article is to provide an analysis of the iconographic sources used in the depiction of Adam and Eve in the 3rd–4th century wall paintings. The scene of the Original Sin initially appeared in wall painting, with the oldest examples dating back to the 3rd century AD. In the following century, images of Adam and Eve were used in both sarcophagus sculpture and crafts. The author of the article compares two different iconographic sources, which together shape the final appearance of Original Sin in the art of the period. A crucial literary source beside the text of Genesis is the apocryphal Lives of Adam and Eve, tracing a different sequence of events related to the story of the Original Sin. On the other hand, the analysis of ancient depictions of Hercules and the goddess Venus reveals an intriguing connection between the representations of the Original Sin and pagan iconography.