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Effect of a single-dose parenteral selenium supplement administered to pregnant dairy cows on selenium and iron concentrations and immune status of calves

K. Żarczyńska A. Snarska L. Rytel P. Sobiech
Polish Journal of Veterinary Sciences | 2018 | vol. 21 | No 2 | 401-403 | DOI: 10.24425/119045
Keywords calves cows selenium IgG GGT passive transfer
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Abstract

The study was performed on 21 H-F calves divided into 3 groups of 7 animals each. The first group was composed of calves whose mothers did not receive an injection of Se preparation. The second and third groups consisted of calves whose mothers were administered injections of Se and vitamin E in a single dose of 10 ml and 30 ml, 10 days before the expected parturition date. 24 hours after birth, blood samples were collected from all calves to determine Se, Fe and IgG concentrations and the activity of GSH-Px and GGT. The results of the study indicate that the administration of a single-dose Se supplement to cows in late pregnancy increases Se concentration in calves and promotes passive transfer of immunity from the mother to offspring.
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Authors and Affiliations

K. Żarczyńska
A. Snarska
L. Rytel
P. Sobiech

Antibody level comparison after porcine epidemic diarrhea vaccination via different immunization routes

C. Hu X. Xie D. Zhao H. Liu X. Liu T. Yang W. Sun
Polish Journal of Veterinary Sciences | 2024 | vol. 27 | No 1 | 143-146 | DOI: 10.24425/pjvs.2024.149342
Keywords porcine epidemic diarrhea IgG IgA neutralizing antibody
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Abstract

Porcine epidemic diarrhea (PED) is a disease extremely harmful to pig health. Intramuscular and Houhai acupoint injections are the main immunization routes to prevent and control PED. This study aimed to evaluate the efficacy of these two routes in pregnant sows based on serum IgG, IgA, and neutralizing antibody levels. PED virus (PEDV) immunoprophylaxis with live-attenuated and inactivated vaccines was administered. The vaccinations for the intramuscular injections elevated IgG and neutralizing antibody levels more than Houhai acupoint injections at most timepoints after immunization. However, the anti-PEDV IgA antibodies induced by vaccination with the two immunization routes did not differ significantly. In conclusion, intramuscular injections are better than Houhai acupoint injections for PEDV vaccination of pregnant sows.
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Bibliography

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Authors and Affiliations

C. Hu
1
X. Xie
2
D. Zhao
3
H. Liu
1
ORCID: ORCID
X. Liu
4
T. Yang
5
W. Sun
6
  1. Pulike Biological Engineering Inc., Luoyang, Henan, 471000, China
  2. Yiyang Vocational and Technical College, Yiyang, Hunan, 413055, China
  3. College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, 410128, China
  4. Xiangtan Center for Animal Disease Prevention and Control, Xiangtan, Hunan, 411104, China
  5. College of Life Sciences and Resource Environment, Yichun University, Yichun, Jiangxi, 336000, China
  6. Sinopharm Animal Health Corporation Ltd., Wuhan, Hubei, 430075, China

Immuno-affinity chromatography for purification of IgG from hyper-immune sera raised against 146S fraction of Foot and Mouth Disease Virus for diagnostic purposes

A. Munir A.A. Anjum I. Altaf A.R. Awan
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 3 | 393–399 | DOI: 10.24425/pjvs.2023.145045
Keywords enzyme linked immuno-sorbent assay (ELISA) Foot and Mouth disease virus (FMDV) hyper-immune sera (HIS) immunoglobulin G (IgG) immuno-affinity chromatography (IAC) protein-A size exclusion chromatography (SEC)
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Abstract

Immunoaffinity chromatography (IAC) is a fundamental isolation and purification tool which is incorporated in a substantial range of therapeutic and diagnostic applications. This study has reappraised the usefulness of immunoaffinity chromatography for the purification of polyclonal antibodies. Protein A based IAC is a convenient and reliable method for purification of IgG, from hyperimmunesera (HIS) raised in experimental animals such as rabbits, guinea pigs and mice to be utilized in pharmaceutics and diagnostics. The 146S fraction of Foot and Mouth Disease virus (FMDV) TCID50=10 5.6 was cultured on a baby hamster kidney cell line 21 (BHK-21), concentrated using salt precipitation method using PEG 6000, purified by size exclusion chromatography (SEC) using Sepharose-30 at 254nm absorbance. Purification of 146S FMDV was analyzed using 12% SDS-PAGE which provided two bands of light and heavy chains. The alum-based vaccine, consisting of ≥10μg of 146S FMDV, was applied in 10 male rabbits and 10 male guinea pigs and two animals of each group were taken as a negative control. The titer of serum was calculated using virus neutralization test. A Protein-A kit (Thermo scientific- 44667, 0528.2) was used to purify HIS raised against 146S FMDV and validated using 12% SDS PAGE in reducing condition. The data demonstrate that protein-A affinity chromatography is an efficient tool for the purification of antibodies from hyper-immune sera raised against 146S FMDV and can be used for the production of diagnostic kits e.g. Enzyme linked immuno-sorbent assay (ELISA) and radioimmunoassay.
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Authors and Affiliations

A. Munir
1
A.A. Anjum
1
I. Altaf
2
A.R. Awan
3
  1. Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Outfall road, Lahore, Pakistan
  2. Quality Operations Laboratory, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Outfall road, Lahore, Pakistan
  3. Department of Biochemistry and Biotechnology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Outfall road, Lahore, Pakistan

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