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Number of results: 9
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Abstract

53 soil samples collected in the Bellsund Region in Western Spitsbergen were examined. An acid-resistant strain difficult to identify was isolated and recognized as Mycobacterium friburgensis. 54.7% of isolated strains were acid-resistant and growing at 25˚C only. They were microorganisms at borderline of Mycobacteria and Actinomycetes. Other microorganisms isolated in the studied soil samples were bacilli (55.7%) and coccaceae (15.4%).

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Authors and Affiliations

Mieczysław Janowiec
Zofia Andrzejczyk
Anna Jezierska-Anczuków
Zbigniew Jóźwik
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Abstract

The aim of this study was to investigate the antimycobacterial activity of 39 free terpenes and their activity in combination with streptomycin. Antimicrobial activity was first evaluated by screening 39 free terpenes at concentrations from 1.56 to 400 μg/mL. None of these exhibited positive effects against any of the nontuberculous mycobacteria (NTM) strains tested. However, six of the 39 terpenes (isoeugenol, nerol, (+)-α-terpineol, (1R)-(−)-myrtenol, (+)-terpinen-4-ol, and eugenol) were shown to enhance the activity of streptomycin against the NTM strains isolated from diseased ornamental fish.
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Bibliography

1. Barbosa CR, Scherf JR, de Freitas TS, de Menezes IR, Pereira RL, dos Santos JF, de Jesus SS, Lopes TP, Silveira Z, Oliveira-Tintino CD, Junior JP, Coutinho HD, Tintino SR, da Cunha FA (2021) Effect of carvacrol and thymol on NorA efflux pump inhibition in multi-drug-resistant (MDR) Staphylococcus aureus strains. J Bioenerg Biomembr 53: 489-498.
2. Cantrell CL, Franzblau SG, Fischer NH (2001) Antimycobacterial plant terpenoids. Planta Med 67: 685–694.
3. Coêlho ML, Ferreira JH, de Siqueira-Júnior JP, Kaatz GW, Barreto HM, de Carvalho Melo Cavalcante AA (2016) Inhibition of the NorA multi-drug transporter by oxygenated monoterpenes. Microb Pathog 99: 173–177.
4. Decostere A, Hermans K, Haesebrouck F (2004) Piscine mycobacteriosis: A literature review covering the agent and the disease it causes in fish and humans. Vet Microbiol 99: 159-166.
5. Dias KJ, Miranda GM, Bessa JR, de Araújo AC, Freitas PR, Almeida RS, Paulo CL, Neto JB, Coutinho HD, Ribeiro-Filho J (2022) Terpenes as bacterial efflux pump inhibitors: A systematic review. Front Pharmacol 13: 953982.
6. De Rossi E, Aínsa JA, Riccardi G (2006) Role of mycobacterial efflux transporters in drug resistance: an unresolved question. FEMS Microbiol Rev 30: 36–52.
7. Gupta, AK, Chauhan DS, Srivastava K, Das R, Batra S, Mittal M, Goswami P, Singhal N, Sharma VD, Venkatesan K, Hasnain SE, Katoch VM (2006) Estimation of efflux mediated multi-drug resistance and its correlation with expression levels of two major efflux pumps in mycobacteria. J Commun Dis 38: 246–254.
8. Guz L, Grądzki Z, Krajewska M, Lipiec M, Zabost A, Augustynowicz-Kopeć E, Zwolska Z, Szulowski K (2013) Occurrence and anti-microbial susceptibility of Mycobacterium peregrinum in ornamental fish. Bull. Vet Inst Pulawy 57: 489-492.
9. Guz L, Puk K (2022) Antibiotic susceptibility of mycobacteria isolated from ornamental fish. J Vet Res 66: 69-76.
10. Jin J, Zhang JY, Guo N, Sheng H, Li L, Liang JC, Wang XL, Li Y, Liu MY, Wu XP, Yu L (2010) Farnesol, a potential efflux pump in-hibitor in Mycobacterium smegmatis. Molecules 15: 7750-7762.
11. Kumar G, Karthik L, Bhaskara Rao KV (2011) Haemolytic activity of Indian medicinal plants toward human erythrocytes: an in vitro study. Elixir Appl Botany 40: 5534-5537.
12. Kumar M, Singh SK, Singh PP, Singh VK, Rai AC, Srivastava AK, Shukla L, Kesawat MS, Jaiswal AK, Chung SM, Kumar A (2021) Potential anti-Mycobacterium tuberculosis activity of plant secondary metabolites: Insights with molecular docking interactions. Antioxi-dans 10: 1990.
13. Mahizan NA, Yang SK, Moo CL, Song AA, Chong CM, Chong CW, Abushelaibi A, Lim SH, Lai KS (2019) Terpene derivatives as a potential agent against antimicrobial resistance (AMR) pathogens. Molecules 24: 2631.
14. Marquez B (2005) Bacterial efflux systems and efflux pumps inhibitors. Biochimie 87: 1137-1147.
15. Martins A, Vasas A, Viveiros M, Molnár J, Hohmann J, Amaral L (2011) Antibacterial properties of compounds isolated from Car-pobrotus edulis. Int J Antimicrob Agents 37: 438-444.
16. Muniz DF, dos Santos Barbosa CR, de Menezes IR, de Sousa EO, Pereira RL, Júnior JT, Pereira PS, de Matos YM, da Costa RH, de Morais Oliveira-Tintino CD, Coutinho HD, Filho JM, de Sousa GR, Filho JR, Siqueira-Junior JP, Tintino SR (2021) In vitro and in sil-ico inhibitory effects of synthetic and natural eugenol derivatives against the NorA efflux pump in Staphylococcus aureus. Food Chem 337: 127776.
17. Puk K, Banach T, Wawrzyniak A, Adaszek Ł, Ziętek J, Winiarczyk S, Guz L (2018) Detection of Mycobacterium marinum, M. pere-grinum, M. fortuitum and M. abscessus in aquarium fish. J Fish Dis 41: 153-156.
18. Puk K, Guz L (2020) Occurrence of Mycobacterium spp. in ornamental fish. Ann Agric Environ Med 27: 535-539.
19. Puk K, Guz L (2022) Effect of alkaloid berberine on the susceptibility of nontuberculous mycobacteria to antibiotics. Pol J Vet Sci 25: 479-481.
20. Puk K, Wawrzykowski J, Guz L (2023) Evaluation of the anti-mycobacterial activity and composition of Carlina acaulis L. root extracts. Pol J Vet Sci 26: 57-63.
21. Sieniawska E, Sawicki R, Swatko-Ossor M, Napiorkowska A, Przekora A, Ginalska G, Augustynowicz-Kopeć E (2018) The effect of combining natural terpenes and antituberculous agents against reference and clinical Mycobacterium tuberculosis strains. Molecules 23: 176.
22. Sieniawska E, Swatko-Ossor M, Sawicki R, Skalicka-Woźniak K, Ginalska G (2017) Natural terpenes influence the activity of antibiot-ics against isolated Mycobacterium tuberculosis. Med Princ Pract 26: 108-112 .
23. Szmygin-Milanowska K, Grzywa-Celińska A, Zwolska Z, Krawczyk P, Guz L, Milanowski J (2016) ‘TB or not TB’ Problems of dif-ferentia diagnosis of cutaneous mycobacteriosis and tuberculosis – A case study and interdisciplinary discussion. Ann Agric Environ Med 23: 97-102.
24. Vasconcelos SS, Caleffi-Ferracioli KR, Hegeto LA, Baldin VP, Nakamura CV, Stefanello TF, Gauze GF, Yamazaki DA, Scodro RB, Siqueira VL, Cardoso RF (2018) Carvacrol activity and morphological changes in Mycobacterium tuberculosis. Future Microbiol 13: 877–888.
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Authors and Affiliations

L. Guz
1
K. Puk
1
D. Szwajgier
2
A. Pastuszka
1

  1. Department of Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
  2. Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland
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Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of infectious enteritis called paratuberculosis that has a high economic impact on the worldwide livestock production. A central important question arises: Can wildlife animals serve as a reservoir for transmission of MAP to domestic ruminants? With this in mind, we devised a study to detect MAP in various Slovakian wildlife species found in the areas where paratuberculosis had been documented in domestic ruminants. The samples of parenchymatous organs (intestines, ileocecal valve and mesenteric lymphatic nodes) from 83 wildlife animals representing 13 species, inclu- ding 7 herbivorous, 5 carnivorous and 1 omnivorous species were collected during a four-year period. The clinical and pathological examinations failed to demonstrate any manifestations of paratuberculosis in any of the wildlife samples. The detection of MAP was done by widely used tests, i.e. cultivation and the PCR analysis. The bacterial cultures revealed the growth of Mycobacterium spp. colonies in 58 (70%) of all of the wild animals, but the PCR testing demonstrated paratuberculosis only in one (7.69%) of the roe deer population.

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Authors and Affiliations

J. Čurlík
P. Lazár
A. Iglódyová
E. Barbušinová
Ľ. Šmiga
J. Novotný
J. Mojžišová
A. Ondrejková
R. Hromada
D. Konjević
M.R. Bhide
M. Drážovská
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Abstract

Nontuberculous mycobacteria (NTM) have recently emerged as important bacterial pathogens of animals and humans. Of particular concern is the high level of antimicrobial resistance displayed by these organisms, which complicates treatment and potential successful outcomes. Here, we evaluated the potential of Carlina acaulis L. as a source of novel anti-mycobacterial agents. Our goal was to measure the activity of aqueous, ethanol, and chloroform C. acaulis root extracts against 99 NTM strains. GC-MS spectroscopy analyses were performed to deliver qualitative and quantitative data on the composition of C. acaulis extract. In our study, we have shown for the first time the activity of C. acaulis extracts against NTM. The highest activity was exhibited by the chloroform extract, which inhibited the growth of more than 90% of the strains at the dose of 100 μg/mL (MIC90 = 100 μg/mL). The results of the GC-MS analysis of the C. acaulis chloroform extract contributed to the identification of 37 compounds, with carlina oxide as the most representative compound (69.52%) followed by 3,4-dihydro-2H-phenanthren- -1-one (6.54%) and stigmast-5-en-3-ol (4.14%). Our results indicate that C. acaulis chloroform and ethanol extracts have potential for treatment of NTM infections and that this plant contains anti-mycobacterial compounds.
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Authors and Affiliations

K. Puk
1
J. Wawrzykowski
2
L. Guz
1

  1. Department of Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland
  2. Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Science in Lublin, Akademicka 12, 20-033 Lublin, Poland
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Abstract

Nontuberculous mycobacteria (NTM) have recently emerged as important bacterial pathogens of both animals and humans. In this study, we aimed to evaluate the effect of a combination of ten antibiotics with an inhibitor of efflux pumps (EPI), i.e. berberine (BER), against 6 strains of NTM. Our results showed that the BER potentiated the anti-mycobacterial activities of the antibiotics. Overall, our findings show the importance of BER in increasing the efficacy of antibiotics in NTM.
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Bibliography


Gaba S, Saini A, Singh G, Monga V (2021) An insight into the medicinal attributes of berberine derivatives: a review. Bioorg Med Chem 38: 116143.
Gentry EJ, Jampani HB, Keshavarz-Shokri A, Morton MD, Velde DV, Telikepalli H, Mitscher LA, Shawar R, Humble D, Baker W (1998) Antitubercular natural products: berberine from the roots of commercial hydrastis canadensis powder. Isolation of inactive 8-oxotetrahydrothalifendine, canadine, beta-hydrastine, and two new quinic acid esters, hycandinic acid esters-1 and -2. J Nat Prod 61: 1187-1193.
Guz L, Puk K (2022) Antibiotic susceptibility of mycobacteria isolated from ornamental fish. J Vet Res 66: 69-76.
Menichini M, Lari N, Rindi L (2020) Effect of efflux pump inhibitors on the susceptibility of Mycobacterium avium complex to clarithromycin. J Antibiot 73: 128-132.
Puk K, Guz L (2020) Occurrence of Mycobacterium spp. in ornamental fish. Ann Agric Environ Med 27: 535-539.
Song L, Wu X (2016) Development of efflux pump inhibitors in antituberculosis therapy. Int J Antimicrob Agents 47: 421-429.
Van Ingen J, Boeree MJ, Van Soolingen D, Mouton JW (2012) Resistance mechanisms and drug susceptibility testing of nontuberculous mycobacteria. Drug Resist Updat 15: 149-161.
Wang Y, Fu H, Li Y, Jiang J, Song D (2012) Synthesis and biological evaluation of 8-substituted berberine derivatives as novel an-ti-mycobacterial agents. Acta Pharm Sin B 2: 581-587.
Zhou XY, Ye XG, He LT, Zhang SR, Wang RL, Zhou J, He ZS (2016) In vitro characterization and inhibition of the interaction between ciprofloxacin and berberine against multidrug-resistant Klebsiella pneumoniae. J Antibiot 69: 741-746.
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Authors and Affiliations

K. Puk
1
L. Guz
1

  1. Department of Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland
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Abstract

Bovine tuberculosis (BTB) is a dangerous zoonosis which presents a serious problem for endangered species such as European bison ( Bison bonasus). Little is known about the influence of parasitic co-infections on the course and diagnosis of tuberculosis in animals. The best known co-infection in cattle is Fasciola hepatica and Mycobacterium bovis. The aim of this study was to review the most recent literature regarding tuberculosis and parasite co-infection in ungulates and relate the results to European bison. Our findings indicate that any comprehensive diagnosis of BTB should include parasitological monitoring, and the possible impact of such invasions on cellular response-based tuberculosis tests should be taken into account. The diagnosis of BTB is complex, as is its pathogenesis, and parasitic infestations can have a significant impact on both. This should be taken into account during further research and monitoring of tuberculosis in European bison.
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Authors and Affiliations

M. Gałązka
1 2
A. Didkowska
1
K. Anusz
1
A. Pyziel-Serafin
1

  1. Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland
  2. Municipal Zoological Garden in Warsaw, Ratuszowa 1/3 03-461, Warsaw, Poland
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Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis mainly in domestic and wild ruminants; paratuberculosis is also known as Johne’s disease. This disease is endemic all over the world generating significant economic losses, especially in dairy herds, although, MAP is the cause of infection in many other species including primates. Currently, MAP mycobacteria are recognized as pathogens transmitted by food. They are a potential threat to animal and human health. Infected animals excreting mycobacteria with faeces are the main source of MAP. The development of control strategies and disease control are based on determi- nation of the genetic diversity of the MAP strains causing Johne’s disease. This study describes 43 strains isolated from a herd of dairy cows located in northern Poland. The types of MAP were determinted based on the polymorphism analysis of two insertion fragments: IS900 and IS1311. The polymorphism of IS900 was analyzed with the use of a PCR multiplex according to Collins’ method and the IS1311 polymorphism with the use of the PCR-REA method. Based on the diffe- rences observed, the strains isolated were classified into two MAP types, cattle (C-type) and sheep (S-type), with the predominance of the cattle type.

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Authors and Affiliations

J. Szteyn
K. Liedtke
A. Wiszniewska-Łaszczych
B. Wysok
J. Wojtacka

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