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Abstract

Measurements of spectral reflectance from potato plants were carried out in the years 2000 and 2001 using the field radiometer CE 313 of Cimel Electronique Company. Field experiments permitted to perceive differences in the reflectance of electromagnetic radiation from potato plant cultivars Bekas and Mila as well as differences between the plants treated with fungicides providing the protection against Phytophthora infestans and the untreated plants. A differentiation of the values of vegetative indices between potato cultivars resulted from the unequal development rate of the cultivars and from their different susceptibility to Phytophthora infestans. The assessment of potato plants infection by the studied pathogen using spectral measurements agreed with the results of field inspection.

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Authors and Affiliations

Andrzej Wójtowicz
Marek Wójtowicz
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Abstract

Phytophthora citricoia dominated among 11 genera and fungal species isolated from Rhododendron brachycarpum, R. catawbiense, R. impeditum and R. sepedonicum. In greenhouse trial isolates from Abies concolor, Chamaecyparis lawsoniana, R. catawbiense, R. impeditum and Thuya occidentalis caused dieback of rhododendron. Inoculation of leaf blades with isolates of the pathogen from 4 cultivars resulted in the spread of necrosis about 0.63 mm/hr. P. citricola was pathogenic to all tested rhododendron cultivars.
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Authors and Affiliations

Leszek B. Orlikowski
Grażyna Szkuta
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Abstract

Common alder (Alnus glutinosa) decline has been observed in most of European countries since 1993. In Poland decline of alder trees has been observed during the last 6 years. Alder Phytophthora was recorded, however, only from one sampling area in the middle of the country. Species of Armillaria, Fusarium, Mucor, Penicillium and Trichoderma were also isolated from diseased trees. Inoculation of alder stem pans, leaves and seedlings with Phytophthora isolates resulted in the development and spread of necrosis. Studies will be continued in the nearest years.
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Authors and Affiliations

Leszek B. Orlikowski
Tomasz Oszako
Grażyna Szkuta
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Abstract

Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens, P. chlororaphis, and P. putida, respectively, among the most represented. More than 36% of bacteria showed antagonistic potential through the production of both diffusible and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For the pot experiment, treatment by Pseudomonas significantly increased the enzymatic activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface, fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas have an intriguing and undeniable potential in the fight against P. colocasiae, which could lead to the development of a biopesticide in the future.
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Authors and Affiliations

Samuel Arsène Ntyam Mendo
1
ORCID: ORCID
Dorice Nguelo Dzumafo
2
Laure Brigitte Kouitcheu Mabeku
3
Severin Tchameni Nguemezi
2
ORCID: ORCID
Lambert Sameza Modeste
2
Rosalie Anne Ngono Ngane
2

  1. Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon
  2. Department of Biochemistry, Faculty of Sciences, University of Douala, Douala, Cameroon
  3. Department of Microbiology, Faculty of Science, University of Yaoundé 1, Yaounde, Cameroon
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Abstract

Phytophthora cryptogea was isolated from diseased stem base of Aquilegia discolor and rotted leaves of Saxifraga and Sempervivum spp. Additionally, Fusarium species and Botrytis cinerea were frequently isolated from diseased parts of plants. Most of Sempervivum species and cultivars except S. soculiferum were colonized in laboratory conditions by P. cryptogea. The isolates from Alstroemeria aurantiaca, Gerbera jamesonii, Saxifraga arendsii, S. paniculata, Sempervivum arachnoideum colonised houseleek leaves with the fastest spread of necrosis on plant parts inoculated with cultures from Gerbera and S. arendsii. The isolate from S. arendsii colonized 5 species and cultivars of saxifrage as well as Iberis sempervivum, Lavendula angustifolia, Sempervivum sobuliferum and Vinca minor with the slowest development on periwinkle. In laboratory trials isolate of P. cryptogea from houseleek grew on PDA and colonized leaves of that plant at temperature ranging from about 10 to 32.5°C with optimum at 20–30°C.

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Authors and Affiliations

Leszek B. Orlikowski
Magdalena Ptaszek
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Abstract

Phytophthora root and stem rot of soybean is a destructive disease of soybean in Iran. During 1998–2005, 142 isolates from soil and diseased soybean plants were collected and tested. Race identification was made possible by inoculating Rps differential soybean cultivars and lines. Of the 142 isolates tested, 110 isolates belonged to race 1 and 32 isolates belonged to race 3. Race 1 was domi nant in soil and diseased plant samples. There was no variability in virulence of Phytophthora sojae between the areas surveyed.

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Authors and Affiliations

Abbas Mohammadi
Azizollah Alizadeh
Mansore Mirabolfathi
Naser Safaie
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Abstract

Genotypic differentiation among 10 isolates of Phytophthora cinnamomi Rands and 24 isolates of Phytophthora citricola Sawada from 12 different plant species grown in Polish ornamental nurseries was determined. DNA was extracted from pure pathogen cultures and amplified by the PCR technique using ISSR and RAPD primers. 9 primers were used to amplify P. cinnamomi and 8 to amplify P. citricola DNA. The analyzed amplification products were between 300 and 2300 bp. The genotypical differentiation was from 17 to 35% in P. cinnamomi and from 10 to 60% in P. citricola. Isolates from host plants of the same family showed, with some exceptions, similar levels of differentiation.

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Authors and Affiliations

Katarzyna Wiejacha
Aleksandra Trzewik
Leszek B. Orlikowski
Grażyna Szkuta
Teresa Orlikowska
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Abstract

This study describes a newly developed index for predicting and forecasting the first (and potentially subsequent) timing of fungicide application against late blight in potato crops based on weather variables measured close to the crop. Inputs for index calculation were the following: daily minimum temperature, mean relative air humidity and daily precipitation. The decisive moment in the process of forecasting is the sum of daily index values for the previous 5 days. The index was tested in various localities of the Czech and the Slovak Republics for several years with a relatively high success rate exceeding the accuracy of previously applied strategies – NoBlight and negative prognosis. In comparison to the mentioned methods, the calculated index corresponded very well to long-term wet periods and indicated the first application date correctly. In years with no wet periods (in this case, 2015 and 2017), it allowed postponing the first application and reducing the number of required sprays during the growing season. The method does not depend on determining the emergence date, so it can be presented on the internet without cooperation with specific growers in a given locality, and thus supply information for a wider range of users. With knowledge about crop development and the degree of resistance to late blight of grown varieties, users can subsequently choose a specific fungicide and its application date.

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Authors and Affiliations

Tomas Litschmann
Ervin Hausvater
Petr Dolezal
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Abstract

The aims of this study were to prospect Penicillium and Talaromyces species associated with Austrocedrus chilensis in sites with and without Austrocedrus Root Disease (ARD), which is caused by the pathogen Phytophthora austrocedri; and to find Penicillium sp. strains with biocontrol activity against this disease. Isolations from sites with and without the prevalence of ARD were made, and the obtained isolates were phenotypically and molecularly identified to species level. Several Penicillium species were isolated from all sites; five of them were exclusively isolated from sites with ARD. Penicillium communities were compared through multivariate analyses; communities were more complex in sites with ARD. Biocontrol activity of Penicillium and Talaromyces isolates was evaluated using co-cultures with Ph. austrocedri. Penicillium glabrum, T. amestolkiae, P. palitans, and P. crustosum showed the highest biocontrol effect. Our results highlight that Penicillium species have high biocontrol activity, which also reinforces the hypothesis that some Penicillium species could be used in formulating a control strategy.
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Authors and Affiliations

Jorge Ariel Marfetán
1 2
ORCID: ORCID
María Laura Vélez
3 4
ORCID: ORCID
Ricardo Comerio
5
ORCID: ORCID
Ana Gallo
1 6
ORCID: ORCID
Stella Romero
2
ORCID: ORCID

  1. Phytopathology and Applied Microbiology, Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), Argentina
  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
  3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Phytopathology and Applied Microbiology, Centro de Investigación y Extensión Forestal Andino Patagónico (CIEFAP), Argentina
  4. Facultad de Ingeniería y Facultad de Ciencias Naturales y Ciencias de la Salud, Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB), Argentina
  5. Estación Experimental Agropecuaria Anguil “Ing. Agr. Guillermo Covas”, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina
  6. Fondo para la Investigación Científica y Tecnológica (FONCYT), Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+I), Argentina
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Abstract

Phytophthora cambivora was isolated from the bark lesions of two 10- and 15-year-old of analysed alder trees. Additionally, Botrytis cinerea, 3 Fusarium species, Mucor spp., P. alni and Trichoderma spp. were recovered from diseased tissues. Isolates of P. cambivora from six plant species, used for inoculation of alder seedlings and plant parts, cause dthe development of necrosis. Isolate from Chamaecyparis lawsoniana was the weakest pathogen whereas those from Abies alba, Acer pennsylvanicum and Alnus glutinosa were the strongest.

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Authors and Affiliations

Leszek B. Orlikowski
Tomasz Oszako
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Abstract

Phytophthora citricola was isolated from diseased seedlings of European beech and Silver fir taken from the most of surveyed nurseries. Fusarium species, Pythium ultimum and Rhizoctonia solani were also found in diseased plant tissues.Isoates of P. citricola fro mboth plants and additionally from heather and rhododendron colonised leaf blades, needles and stem parts of beech and fir. In greenhouse trials on inoculated 1-year-old seedlings necrosis spread about 2 mm/24 hr on beech stems whereas on fir about 1.5 mm/24 hr.

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Authors and Affiliations

Leszek B. Orlikowski
Barbara Duda
Grażyna Szkuta
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Abstract

In the field experiments performed in 1999–2001 the profitability of late blight control in accordance with three decision support systems: NegFry, Simphyt and Stephan with routine fungicide program was compared. Potato protection carried out according to the recommendations of the decision support systems guaranteed higher profitability of late blight control than when potato was protected routinely. The highest profitability was recorded for susceptible variety Bekas protected according to NegFr.

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Authors and Affiliations

Andrzej Wójtowicz
Jerzy J. Lipa
Erich Jörg
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Abstract

Phytophthora citricola Sawada was detected from 3 water pools situated in 2 container- grown nurseries. The highest number of spots on rhododendron leaves were observed in June whereas the lowest in October. The use of water for plant sprinkling caused browning, yellowing of shoots and root rot of Buxus sempervirens, and blight of shoot tips of Thuja occidentalis and Rhododendron sp. The disease symptoms were observed already in June and the disease developed till the first decade of October. Losses caused by the species varied from 9 to 56%.

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Authors and Affiliations

Leszek B. Orlikowski
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Abstract

Research over a three year period indicated that P. ramorum occurred rarely in Poland on Rhododendron spp., in spite of established monitoring in nurseries, trade stands, forest and water from early spring to late autumn each year. The pathogen was not found in forests on Vaccinium vitisidaea, Calluna vulgaris, Fagus sylvatica and Quercus rubra, proving its limited spread. The species was detected, however, from 2 rivers. P. citricola was isolated from most of surveyed plants. Besides this P. cactorum, P. cinnamomi, P.citrophthora and P. nicotianae var. nicotianae were isolated from diseased plants. Additionally Pestalotia sydowiana, species of Fusarium, Botrytis cinerea and Trichoderma were often found in diseased plant tissues. Laboratory and glasshouse research showed slight differences in colonization of plants by P. ramorum and P. citricola. However, taking into account the range of host plants, and frequency of pathogen occurrence in infected plant material and water, it became clear that P. citricola poses a much greater danger than P. ramorum to the natural environment in Poland.

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Authors and Affiliations

Leszek B. Orlikowski
Tomasz Oszako
Aleksandra Trzewik
Teresa Orlikowska
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Abstract

From Hedera helix and Epipremnum aureum showing necrosis of shoot base spread upwards and on leaves Phytophthora tropicalil was isolated. The species was obtained from ⅞of Hedera and ¾ of Epipremnum diseased shoot and root parts. Additionally, Botrytis cinerea, Fusarium avenaceum and Rhizoctonia solani was recovered from some of affected plants. The chosen 2 isolates colonised petioles and leaf blades of both host plants. P. tropicalis caused necrosis of leaves of 11 tested cultivars of H. helix and 13 other pot plant species and seedlings of tomato. The fastest spread of necrosis was observed on leaves of Peperomia magnoliaefolia, Pelargonium zonale and Phalaenopsis x hybridum. The development of disease was observed at temperatures ranged from 10 to 32.5°C with optimum 30°C.

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Authors and Affiliations

Leszek B. Orlikowski
Aleksandra Trzewik
Katarzyna Wiejacha
Grażyna Szkuta
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Abstract

Phytophthora citricola constituted about 70% of all Phytophthora isolates recovered from rhododendron leaves used as baits for detection of that group of organisms in water. The species was found in 4 rivers, 2 hardy nursery water reservoirs and nursery drainage canal from May to October, 2006. Analysis of spots’ number on rhododendron leaf baits as a measure of P. citricola density showed that place of holding baits had a significant influence on the species occurrence. Significantly more spots, especially in July survey, were observed on baits held in Skierniewka and Zwierzynka rivers swimming through agricultural and forest area than in Ner, the river of horticultural area. Significantly more spots/rhododendron leaves were observed when they were held in rivers downstream of nursery and in the middle of hardy nursery borders. In nursery water containers and drainage canal higher Phytophthora density was recorded in August than in other periods of surveying. Using water from reservoir for sprinkling of Picea omorika nursery trees caused the development of tip blight and from diseased twigs P. citricola was isolated.

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Authors and Affiliations

Leszek B. Orlikowski
Aleksandra Trzewik
Teresa Orlikowska
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Abstract

A survey was conducted in February of 2004 on the outbreak of stem rot and wilt disease of pepper at the Kitabawa/Danzakara and Ajiwa irrigation sites in Northern Nigeria. Laboratory investigations revealed that it was elicited by Phytophthora capsici Leon. The disease caused severe loss in yield and USD 1 700.00 to USD 3 200.00 loss in revenue/ha. The disease was probably further aggravated by the presence of Fusarium sp. as well as ecto- and endoparasitic nematodes. Reasons for outbreak were elucidated and solutions proffered.

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Authors and Affiliations

Matthew Alegbejo
Abdulahi Lawal
Paul Chindo
Olalekan Banwo
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Abstract

β-1,3-glucanases play a major role in combating the abnormal leaf fall disease (ALF) caused by the oomycete Phytophthora spp. in Hevea brasiliensis, the major commercial source of natural rubber. In this study, partial sequences of four novel promoters of different β-1,3-glucanase genomic forms were amplified through inverse PCR from the H. brasiliensis clone RRII 105 and sequence characterized. This is the first report showing β-1,3-glucanase genes driven by a different set of promoter sequences in a single clone of Hevea. The nucleotide sequencing revealed the presence of 913, 582, 553 and 198 bp promoter regions upstream to the translation initiation codon, ‘ATG’, and contained the essential cis-elements that are usually present in biotic/abiotic stress-related plant gene promoters along with other complex regulatory regions. The amplified regions showed strong nucleosome formation potential and in two of the promoters CpG islands were observed indicating the tight regulation of gene expression by the promoters. The functional efficiency of the isolated promoter forms was validated using promoter: reporter gene (GUS) fusion binary vectors through Agrobacterium mediated transformation in Hevea callus and tobacco. GUS gene expression was noticed in Hevea callus indicating that all the promoters are functional. The transgenic tobacco plants showed no GUS gene expression. The implication of these novel promoter regions to co-ordinate the β-1,3-glucanase gene expression can be utilized for defense specific gene expression in future genetic transformation attempts in Hevea and in a wide variety of plant systems.
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Authors and Affiliations

Supriya Radhakrishnan
1 2
Suni Anie Mathew
1 3
Alikunju Saleena
1 4
Arjunan Thulaseedharan
1

  1. Advanced Center for Molecular Biology and Biotechnology, Rubber Research Institute of India, Kottayam, Kerala, India
  2. Department of Biotechnology, University of Kerala,Thiruvananthapuram, Kerala, India
  3. Faculty of Science and Engineering, University of Turku, Turku, Finland
  4. Department of Cell Biology and Molecular Medicine, Rutgers New Jersey Medical School, Newark, New Jersey, United States of America
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Abstract

The influence of different nozzle types and adjuvants on the biological efficacy of fungicides in potato was investigated in four growing seasons in the years 1997-2000. The studied foliar plant disease was late blight (Phytophthora infestans). The biological efficacy of fungicides (alone or with adjuvants) applied with coarse air induction nozzles was comparable to fine standard flat fan nozzle. The differences between the nozzle types tested were small in control oflate blight in potato with systemic and contact fungicides. Coverage of upper side of leaf blades was from three to four times higher than lower side of leaf blades. In general, the highest coverage of upper and lower side of leaf was achieved when the full dosage rate of fungicide (copper oxychloride) was used with both the flat fan XR 110-03 and air induction DB 120-03 nozzle. Adding Atpolan 80 EC and Zero Piany 62 SL to 50% dosage rate of copper oxychloride decreased the spray coverage on lower side ofleaf as well as with both the XR 110-03 and DB 120-03 nozzles. However, addition of adjuvants to spray solution did not decrease the deposition on upper side of leaf blade. In general, adjuvants did not considerably influence the biological efficacy of the fungicide treatments sprayed with both the flat fan and the air induction nozzles but had favourable influence on potato yield.
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Authors and Affiliations

Roman Kierzek
Marek Wachowiak
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Abstract

The paper attempts to assess the extent of crop loss in rubber plantations in India, measured in terms of loss in latex and timber output and thereby to examine the comparative economics of plant protection measures against Phytophtora spp. induced abnormal leaf fall (ALF). The specific objectives were: a) to examine the extent of loss in latex and timber output in unsprayed plots vis-a-vis sprayed plots across prominent rubber clones; b) estimate the value of loss in latex and timber output across clones between sprayed and unsprayed plots; c) examine the comparative economics of plant protection measures in terms of the incremental costs and the incremental returns from sprayed plots across clones; and d) reflect upon the policy imperatives with respect to region specific Research and Development (R&D) interventions on plant protection measures in India. The study brings out significant clonal differences in loss of latex and timber output in the absence of prophylactic spraying against ALF. The observed clonal differences with respect to feasibility of plant disease control measures indicate the need for region and clone-specific recommendations for plant protection measures in India instead of the currently followed unilateral prescription with due allowance to the costs and potential benefit accrued from the control measures. The study also highlight the need for evolving interventions and agro-management/ plant protection measures for minimising the incidence of tree casualty in rubber plantation, as it amounts to loss of potential income from latex and timber from rubber plantations in India, dominated by the smallholder sector

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Authors and Affiliations

Pozhamkandath K. Viswanathan
Tharian George Kadavil
Chakkasseril Kuruvilla Jacob

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