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Abstract

In the paper a new method, called the Noise Scattering Pattern (NSP) method, for RTS noise identification in a noise signal is presented. Examples of patterns of the NSP method are included.

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Authors and Affiliations

A. Konczakowska
J. Cichosz
A. Szewczyk
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Abstract

Cross layer cooperative protocol which exploits the benefits of physical layer cooperative communication, is one of the widely recognized MAC layer protocol design strategies for future wireless networks. This paper presents performance analysis of a cooperative mac and these performance parameters are compared those of the legacy IEEE 802.11 DCF MAC. Appropriate relay station selection is the main hurdle in designing efficient cooperative MAC protocol for wireless networks. This cooperative mac demonstrated that intermediate relay nodes themselves can initiate cooperation for relaying data frame to the receiver on behalf of the sender. This procedure makes the selection process of a “helper node” more distributed in nature as well as it contributes to increase throughput of a wireless network by reducing the overheads that are usually incurred in the helper selection process. It has been shown by thorough analytical analysis that the proposed cooperative MAC protocol offers higher throughput and lower frame transmission delay in both ideal and error prone wireless environment. These performance metrics are also evaluated while the wireless nodes are mobile as well.

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Authors and Affiliations

Md. Ruhul Amin
Md. Shohrab Hossain
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Abstract

In the paper a new method of Random Telegraph Signal (RTS) noise identification is presented. The method is based on a standardized histogram of instantaneous noise values and processing by Gram-Charlier series. To find a device generating RTS noise by the presented method one should count the number of significant coefficients of the Gram-Charlier series. This would allow to recognize the type of noise. There is always one (first) significant coefficient (c0) representing Gaussian noise. If additional coefficients cr (where r > 0) appear it means that RTS noise (two-level as well as multiple-level) is detected. The coefficient representing the Gaussian component always has the highest value of all. The application of this method will be presented on the example of four devices, each with different noise (pure Gaussian noise signal, noise signal with two-level RTS noise, noise signal with three-level RTS noise and noise signal with not precisely visible occurrence of RTS noise).

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Authors and Affiliations

Barbara Stawarz-Graczyk
Dariusz Dokupil
Paweł Flisikowski
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Abstract

Maize dwarf mosaic virus (MDMV) is a serious and widespread virus pathogen of maize plants. This +ssRNA virus belongs to the Potyvirus genus in the Potyviridae family. Together with sugarcane mosaic virus (SCMV) it causes one of the most important viral diseases on maize crops in the world – maize dwarf mosaic. Both viruses are transmitted in the same non-persistent manner by several aphid species. They induce similar symptoms of leaf mosaic or mottling, stunting and a reduction in plant weight and grain yield. Available MDMV diagnostics include primarily commercialized enzyme-linked immunosorbent assays (ELISA) and reverse transcription-polymerase chain reactions (RT-PCR). Here, laborsaving reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was optimized for identification of genetically different MDMV isolates. For this purpose, primer sets, MDMVF3/MDMVB3 and MDMVFIP/MDMVBIP amplifying fragments of coat protein coding sequence of MDMV, were used. The specificity of the reaction was verified using three MDMV (-P1, -Sp, -PV0802-DSMZ) and three SCMV (-P1, -PV0368- -DSMZ, -PV1207-DSMZ) isolates. Obtained products were visualised by DNA staining, electrophoretic separation as well as by real-time monitoring of the reaction. The sensitivity of RT-LAMP and conventional RT-PCR reactions was comparable. Both methods could detect virus as low as 550 fg · μl–1 of total RNA. This technique has application value for screening MDMV by phytosanitary services.
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Authors and Affiliations

Katarzyna Trzmiel
1
ORCID: ORCID
Beata Hasiów-Jaroszewska
1
ORCID: ORCID

  1. Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznan, Poland
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Abstract

Numerous plant species around the world suffer from the presence of viruses, which especially in economically important crops, cause irretrievable damage and/or extensive losses. Many biotechnological approaches have been developed, such as meristem culture, chemotherapy, thermotherapy or cryotherapy, to eliminate viruses from infected plants. These have been used alone or in combination. In this work, meristem culture, thermotherapy and cryotherapy were compared for Apple mosaic virus elimination from hazelnut local cultivar “Palaz”. The virus-free plant was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) after each treatment and, the best results were obtained by cryotherapy. A one step freezing technique, droplet vitrification, was used for cryotherapy, and the best regeneration percentage was 52%. After cryotherapy, virus-free seedlings of hazelnut local cultivar “Palaz” were confirmed as being virus-free after three subcultured periods.
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Bibliography

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Authors and Affiliations

Ergun Kaya
1

  1. Molecular Biology and Genetics, Mugla Sitki Kocman University, Mugla, Turkey
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Abstract

The aim of this work was to evaluate the relative gene expression levels of the cytokines IL- 1B, IL-8, IL-12, IFN-γ, IL-4, IL-10 and TGF-β in somatic milk cells of French Alpine breed, anestrous goats that were experimentally infected in the left mammary gland with Staphylococcus chromogenes during the lactation peak. Milk samples were obtained from both glands for 21 consecutive days post infection. Total RNA was extracted, and real-time PCR was conducted using primers specific to each cytokine. The relative RNA expression of the evaluated cytokines was determined by the comparative method 2-ΔΔCT, using milk from the right gland of the goats as a reference (control) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous control. According to the Wilcoxon test results, IL-1B and IL-12 expression levels showed significant differences compared to those in the control group (p<0.05) from 24 hours post infection until the end of lactation; on day three, IL1β, IL8, IL12 and TGF-β had a statistically significant change in expression with respect to those in the control group (p<0.05); closer to the end of the lactation period, there is no overexpression of the anti-inflammatory interleukins (IL-4 and TGF-β) which may reflect the effort of the host immune system to eradicate the microorganism from the mammary gland.

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Authors and Affiliations

R.A. Ruiz-Romero
D. Martínez-Gómez
R.A. Cervantes-Olivares
E. Díaz-Aparicio
A.E. Ducoing-Watty
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Abstract

The use of lactoferrin (LF) and/or lactobacillus sp. (LB) to improve animal health and production has increased recently. However, information regarding the immune-modulatory role of LB supplementations either alone or in combination with LF in sheep remains unclear. Therefore, the present study was designed to evaluate the immune modulating properties and the antioxidant activity of supplementing commercially available LF and/or LB in healthy lambs. For this reason, twenty-four apparently healthy Ossimi lambs were used. After three weeks of acclimatization, the lambs were randomly allocated to four equal-sized groups and assigned to receive one of the following supplements: LB at a dose of ~ 1 g active ingredient/head (group 1), LF at a dose rate of 0.5 gm /head (group 2), a combination of both treatments using the same dosing regimens (group 3), and (group 4) received only 10 mL of isotonic saline and was considered as a control group. All supplements were given orally twice daily for 30 consecutive days. Blood samples were collected from each lamb before starting the experiment (T0) and two weeks (T15), and four weeks (T30) after giving supplements for hematological examinations, serum biochemical analyses, and RT-PCR assays. Our findings demonstrated that lambs receiving LB showed statistically significant (P<0.05) higher values of total leucocytes, lymphocytes and lysozyme activity than those receiving LF. In contrast, lambs that received LF had significantly (P< 0.05) higher values of serum catalase, nitric oxide and GSH with a significantly lower MDA level compared with those supplemented with LB. A combination of LF and LB supplementation elicited maximal up-regulation of Tollip, TLR4, IL-5, and IL-6 gene expression compared with other groups. The results suggest that bovine LF and or LB could be used as useful nutritional supplements to support the immune system in healthy lambs.

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Authors and Affiliations

M. El-Ashker
E. Risha
F. Abdelhamid
A. Ateya
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Abstract

A comparative analysis of a compact planar Square patch Microstrip Multiband antenna on three different substrates is proposed. The proposed design has a C-shaped slot etched on the square radiating part and the antenna is energized using microstrip feed line. RT Duroid (ε r= 2.2), Taconic (ε r= 3.2) and FR4 (ε r= 4.4) substrates are used for simulation analysis. The flow of current is modified by the C-shaped slot making the antenna to resonate at 3/4 and 6 bands for RT Duroid/Taconic and FR4 substrates respectively suitable for 5G sub GHz applications. The antenna has a compact dimension of 32 × 32 × 1.6 mm 3 and exhibits a return loss, S11 of less than -10dB for all the resonating frequencies for all three substrates. The analysis has been done by considering the S11 (Return loss <-10 dB), Directivity, Antenna Gain, VSWR and surface current distribution. Table II provides the comparison of parameters for different substrate material.
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Authors and Affiliations

P. Nagaraju
1
D.H. Sachina
2
Imran Khan
3
H.V. Kumaraswamy
1
K.R. Sudhindra
2

  1. Department of Electronics & Telecommunication Engineering, RVCE, Bangalore, India
  2. Department of Electronics & Communication Engineering, BMSCE, Bangalore, India
  3. Department of Electronics & Communication Engineering, Government Engineering College, Ramanagara, India
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Abstract

From the naturally infected cucumber plane spherical virus was isolated that mainly on basis of its serological properties has been identified as Tomato black ring virus (TBRV). Using antiserum against TBRV-ED for the specific crapping of virus followed by PCR test (immunocapture-RT-PCR) allowed co distinguish TBRV from related viruses, especially Beet ringspot virus (BRSV). Presence of as many as rwo satellite RNAs should be found as a unique feature of the cucumber isolace.
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Authors and Affiliations

Henryk Pospieszny
Magdalena Jończyk
Natasza Borodynko
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Abstract

This article validates the application of RT-Lab for the AGC studies of three-area systems. All the areas are employed with thermal-DSTS systems. A new controller named cascade FOPDN-FOPPIDN is employed. Its parameters are optimized using a CSA, subjecting to a new PI named HPA-ISE. The responses of the FOPDN-FOPIDN controller are related and are superior over PIDN and TIDN controllers. Moreover, the dominance of HPA-ISE is verified with ISE, and it performs better in terms of system dynamics. Further, the system performance reliability is analyzed with the AC-HVDC and is better than the AC system. Besides, sensitivity analysis recommends that the proposed FOPDN-FOPIDN at diverse conditions is robust and more reliability.
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Authors and Affiliations

Naladi Ram Babu
1
Tirumalasetty Chiranjeevi
2
Ramesh Devarapalli
3
ORCID: ORCID
Łukasz Knypiński
4
ORCID: ORCID
Fausto Pedro Garcìa Màrquez
5

  1. Department of Electrical and Electronics Engineering, Aditya Engineering College, Surampalem, Andhra Pradesh, India
  2. Department of Electrical Engineering, Rajkiya Engineering College Sonbhadra, U.P., India
  3. Department of Electrical/Electronics and Instrumentation Engineering, Institute of Chemical Technology, Indianoil Odisha Campus, Bhubaneswar751013, India
  4. Faculty of Control, Robotics and Electrical Engineering, Poznan University of Technology, Piotrowo 3A, 60-965 Poznan, Poland
  5. Ingenium Research Group, University of Castilla-La Mancha, Spain
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Abstract

The absolute positions of shearers on advancing coal faces are requisite for providing references for adaptive mining combined with geological models. Common coalmine localization techniques (e.g. UWB, INS, etc.) are not fully applicable to adaptive mining due to their drifting error or the messy environment. The gyro robotic total station (RTS) is versatile and precise in measuring coordinates in coal mines, while its conventional usage is of low automation and poor timeliness, impeding its application on mining faces. This article proposed an automated gyro RTS system for real-time absolute positioning on fully mechanised coal faces. The measuring process was changed to fit mining requirements, and a new state-transferring model was used to automate it. Programs were developed and installed in available instruments, forming a prototype. Field experiments were carried out on a simulative working face, verifying the system’s accuracy and applicability. Results show that the relative positioning error is better than 2.6143×10-4, which meets the demand of advancing faces. The error of the gyro is estimated at 55.5187”, justifying its nominal indicators. To sum up, the automated gyro RTS system proposed in this paper can offer real-time and accurate absolute positions of equipment on working faces, supporting adaptive mining combined with the geological model.
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Authors and Affiliations

Ben Li
1
ORCID: ORCID
Shanjun Mao
1
ORCID: ORCID
Haoyuan Zhang
1
ORCID: ORCID
Xinchao Li
2
ORCID: ORCID
Huazhou Chen
2
ORCID: ORCID

  1. Peking University, Institute of Remote Sensing and Geographic Informat ion System, Beijing 100871, China
  2. Beijing Longruan Technologies Co., Ltd., Beijing 100871, China
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Abstract

Improvements in water quality requires the removal of nitrogen compounds from wastewater. The most promising and cost-effective methods for this purpose are biological ones based on activated sludge microorganisms such as nitrifiers, denitrifiers, and anammox bacteria. Due to the most of the nitrogen removal bacteria are uncultivable in a laboratory, the application of the molecular tools is required to investigate microorganisms involved in the nitrogen removal. In case of this study for the analysis of relative genes abundance of nitrogen removal bacteria, quantitative PCR (qPCR) based on bacterial DNA and qPCR preceded by reverse transcription (RT-qPCR) based on bacterial mRNA as a template, were used with specific bacterial functional genes ( amoA, nrxA, nirS, nirK, hzo). Samples from four anammox sequencing batch reactors (SBRs) were analyzed, while the nitrogen removal process and bacteria growth were supported by biomass immobilization and nanoparticles addition. There were statistically significant differences between results obtained in the case of mRNA and DNA (p<0.05). Statistically significant positive correlations were found between results obtained with those two approaches. In case of mRNA analysis, positive results were obtained only for hzo, amoA and partly for nirS genes, despite additional purification and removal of inhibitors from samples prior to reaction.
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Authors and Affiliations

Anna Banach-Wiśniewska
1
Filip Gamoń
1
Aleksandra Ziembińska-Buczyńska
1

  1. Silesian University of Technology, Faculty of Power and Environmental Engineering, Environmental Biotechnology Department, Gliwice, Poland
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Abstract

Onion yellow dwarf virus (OYDV), an aphid-borne potyvirus is one of the major viral pathogens of garlic causing significant yield losses worldwide. It is found almost everywhere in the world where Allium species is grown. The aim of this study was to test the presence of OYDV infection in garlic from Ethiopia. The presence of the virus was tested by Reverse transcription polymerase chain reaction (RT-PCR). The direct sequencing of the PCR product produced a sequence of 296 bp. Sequence analysis showed 89.27% sequence homology with an isolate from Australia (HQ258894) and 89.29% with an isolate from Spain (JX429964). A phylogenetic tree constructed with MEGA 7.0 revealed high levels of homology with various isolates of OYDV from all over the world and thus further confirmed the identity of the virus.

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Authors and Affiliations

Yohanis Kebede
Jyoti Singh
Shahana Majumder
ORCID: ORCID
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Abstract

Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterisation of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the coat protein gene ( CP) of the Polish and other DsMV isolates described to date. The presence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse transcription polymerase chain reaction (RT-PCR) with specific, diagnostic primers in three out of ten examined plants. To obtain full-length sequences of CP, two pairs of primers were designed and used in the RT-PCR. The specificity of obtained products was confirmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other DsMV sequences retrieved from the GenBank. Analyses revealed that DsMV population is very diverse. The variability of DsMV isolates was confirmed by low sequence identity and pervasive recombination events. The phylogenetic analysis was performed based on 37 non-recombinant CP sequences. The maximum-likelihood reconstruction revealed that the Polish isolates are distinct and grouped separately from other DsMV isolates. Due to the high genetic diversity, detecting the virus could be difficult. Nonetheless disease management relies strongly on a fast and accurate identification of the causal agent. To our knowledge this is the first report of DsMV in Poland.
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Authors and Affiliations

Agnieszka Taberska
1
Julia Minicka
1
Daria Budzyńska
1
Beata Hasiów-Jaroszewska
1
ORCID: ORCID

  1. Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, Poland
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Abstract

Leaf scald, caused by the necrotrophic fungus Monographella albescens, is one of the main threats to rice (Oryza sativa L.) around the world. This disease decreases yields in rice by up to 30% because of dead leaf tissue, damaged seeds, and sterile flowers. Currently, there is limited knowledge about the molecular mechanisms involved in rice plant resistance against this pathogen. For this purpose, six commercial cultivars of rice were primarily screened for M. albescens infection and development. Dasht and Salari were found to be the most resistant and susceptible to M. albescens infection, respectively. The plants were kept in a greenhouse at 29 ± 2°C during the day and 26 ± 2°C at night with a relative air humidity of 85 ± 5%. Forty-five days after sowing, the plants with three biological replications were inoculated by transferring a PDA disc (0.3 cm2) containing M. albescens mycelia to the middle third of the 7th, 8th, and 9th completely open leaves. The leaves were collected 24, 48, 72, 96 and 120 hai. Leaf samples were also collected from the non-inoculated plants (0 h) to serve as controls. Real-time quantitative PCR (RT-qPCR) showed rapid induction and significant accumulation of jasmonic acid (JA) and ethylene (ET) responsive genes such as lipoxygenase (LOX), allene oxide synthase 2 (Aos2), jasmonic acid carboxyl methyltransferase 1 (JMT1) and ACC synthase 1 (ACS1) in the resistant Dasht cultivar after infection with M. albescens. Furthermore, the transcripts of salicylic acid (SA) responsive phenyl alanine ammonia lyase 1 (PAL1) and nonexpressor of pathogenesis-related genes 1 (NPR1) genes were induced in the incompatible interaction. The activities of the defense enzymes superoxide dismutase (SOD), peroxidase (POX) and glutathione reductase (GR) increased strongly in Dasht in response to M. albescens infection. In addition, there was an increase in the H2O2 levels in the leaves of the Dasht cultivar during the infectious period of M. albescens associated with the enhancement of catalase (CAT) activity as well as higher levels of malondialdehyde (MDA). This is the first study on the interaction between rice and M. albescens at the molecular level. It can contribute to understanding how rice responds to pathogen infection, as well as assist with future research plans of molecular breeding regarding the tolerance to leaf scald disease.

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Authors and Affiliations

Dariush Ebadi Almas
Atefeh Rahmani Kamrodi
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Abstract

Bovine parvovirus (BPV), bovine coronavirus (BCoV) and bovine parainfluenza virus (BPIV) are common etiologies causing gastrointestinal and respiratory diseases in dairy herds. However, there are few reports on the synchronous detection of BPV, BCoV and BPIV. The present article aimed to develop a quick and accurate RT-PCR assay to synchronously detect BPV, BCoV and BPIV based on their specific probes. One pair universal primers, one pair specific primers and one specific probe was designed and synthesized. After the concentrations of primer and probe and annealing temperature were strictly optimized, the specificity, sensitivity and repeatability of the established triplex probe qRT-PCR were evaluated, respectively. The results showed the recombinant plasmids of pMD18-T-BPV, pMD18-T-BCoV and pMD18-T-BPIV were 554bp, 699bp and 704bp, respectively. The optimal annealing temperature was set at 45.0°C for triplex qRT-PCR. The triplex probe qRT-PCR can only synchronously detect BPV, BCoV and BPIV. Detection sensitivities were 2.0×102, 2.0×102 and 2.0×101 copies/μL for BPV, BCoV and BPIV, being 1000-fold greater than that in the conventional PCR. Detection of clinical samples demon- strated that triplex probe qRT-PCR had a higher sensitivity and specificity. The intra-assay and inter-assay coefficient of variation were lower than 2.0%. Clinical specimens verified that the triplex qRT-PCR had a higher sensitivity and specificity than universal PCR. In conclusion, this triplex probe qRT-PCR could detect only BPV, BCoV and BPIV. Minimum detection limits were 2.0×102 copies/μL for BPV and BCoV, and 2.0×101 copies/μL for BPIV. The sensitivity of this triplex probe qRT-PCR was 1000-fold greater than that in the conventional PCR. The newly qRT-PCR could be used to monitor or differentially diagnose virus infection.

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Authors and Affiliations

J. Geng
Y. Niu
L. Wei
Q. Li
Z. Gong
S. Wei
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Abstract

In the spring of 2019, many plants, mainly winter wheat, were observed to have dwarfism and leaf yellowing symptoms. These plants from several regions of Poland were collected and sent to the Plant Disease Clinic of the Institute of Plant Protection – National Research Institute in Poznań to test for the presence of viral diseases. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) results showed numerous cases of Wheat dwarf virus (WDV) and a few cases of plant infections caused by Barley yellow dwarf viruses (BYDVs). WDV was detected in 163 out of 236 tested winter wheat plants (69.1%), in 10 out of 27 tested winter barley plants (37%) and in 6 out of 7 triticale plants (85.7%) while BYDVs were found, respectively, in 9.7% (23 out of 236) and in 18.5% (5 out of 27) of tested winter forms of wheat and barley plants. Infected plants came mainly from the regions of Lower Silesia and Greater Poland. Furthermore, individual cases of infections were also confirmed in the following districts: Lubusz, Opole, Silesia, Kuyavia-Pomerania and Warmia-Masuria. Results of Duplex-immunocapture-polymerase chain reaction (Duplex-IC-PCR) indicated the dominance of WDV-W form in wheat and WDV-B form in barley plants. Moreover, results of reverse transcription – polymerase chain reaction (RT-PCR) connected with restriction fragment length polymorphism (RFLP) analysis, performed for 17 BYDVs samples, revealed 8 BYDV-PAS, 4 BYDV-MAV and 2 BYDVPAV as well as the presence of two mixed infections of BYDV-MAV/-PAS and one case of BYDV-MAV/-PAV. Next, RT-PCR reactions confirmed single BYDV-GAV infection and the common presence of BYDV-SGV. To the best of our knowledge, in 2020 the viruses were not a big threat to cereal crops in Poland.

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Authors and Affiliations

Katarzyna Trzmiel
ORCID: ORCID

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