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Abstract

A biotic interaction between fungi from soil within and outside the rhizosphere of potato and fungi responsible for black scurf – Rhizoctonia solani Kühn and silver scurf – Helminthosporium solani (Dur., Mont.). It was found that fungi population connected with crop environment under investigation promoted the growth of Rhizoctonia solani, thus indicating no resistance of this environment to this pathogen. These fungi, however, inhibited the growth of Helminthosporium solani.

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Authors and Affiliations

Halina Kurzawińska
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Abstract

The effect of biopreparation Biochikol 020 PC on linear growth of mycelia of two Rhizoctonia isolates was tested under laboratory conditions along with that of fungicide Baytan Universal 19,5 WS for comparative purposes. Complete retardation of mycelial growth of both isolates was observed in case the application of fungicide Baytan Universal 19,5 WS at the concentration of 0.1% and a considerable growth inhibition was stated at the temperatures of 10°C and 30°C. The effect of seed dressing with the above mentioned preparations on plant infestation by Rhizoctonia fungi was tested in a greenhouse experiment on two cultivars of spring wheat: Banti and Ismena. Baytan Universal 19,5 WS reduced plant infestation especially at the highest soil moisture content of 20 kPa. Biopreparation Biochikol 020 PC and diversification of soil moisture levels did not result in a reduction of wheat infestation rate. This biopreparation stimulated the increase of root mass of both cultivars at moisture content of 20 kPa.

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Authors and Affiliations

Ewa Żółtańska
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Abstract

Nanodiagonastic methods in plant pathology are used for enhancing detection and identification of different plant pathogens and toxigenic fungi. Improvement of the specificity and efficiency of the polymerase chain reaction (PCR) by using some nanoparticles is emerging as a new area of research. In the current research, silver, zinc, and gold nanoparticles were used to increase the yield of DNA for two plant pathogenic fungi including soil-borne fungus Rhizoctonia solani and toxigenic fungus Alternaria alternata. Gold nanoparticles combined with zinc and silver nanoparticles enhanced both DNA yield and PCR products compared to DNA extraction methods with ALB buffer, sodium dodecyl sulfate, ALBfree from protinase K, ZnNPs and AgNPs. Also, by using ZnNPs and AgNPs the DNA yield was enhanced and the sensitivity of random amplified polymorphic DNA (RAPD) PCR products was increased. Application of nanomaterials in the PCR reaction could increase or decrease the PCR product according to the type of applied nanometal and the type of DNA template. Additions of AuNPs to PCR mix increased both sensitivity and specificity for PCR products of the tested fungi. Thus, the use of these highly stable, commercially available and inexpensive inorganic nano reagents open new opportunities for improving the specificity and sensitivity of PCR amplicon, which is the most important standard method in molecular plant pathology and mycotoxicology.
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Authors and Affiliations

Fahad A. Al-Dhabaan
Heba Yousef
Tahsin Shoala
Jumana Shaheen
Yousra El Sawi
Tasneem Farag
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Abstract

The investigation was conducted in 2004 and 2005 to test 28 sesame genotypes for resistance and susceptablity to Rhizoctonia solani under artificial infection conditions at the Plant Breeding Experimental Farm of the Faculty of Agriculture, Suez Canal University, Ismailia, Egypt. All screened sesame genotypes showed varied significant degrees of infestation with the root rot pathogen. It is worth to mention that some of sesame genotypes kept their resistance characterestic classes as moderately resistant (MR) or resistant (R) during the two successive seasons. Such genotypes might be useful for breeding programs due to stability of their resistant character as well as their seed yield. Phenotypic coefficients of variation (P.C.V.) and genotypic coefficients of variation (G.C.V.) were of high value regarding resistance characters during both seasons and comparable to seed yield character. The heritability estimates indicate that selection is a suitable way for picking up sesame genotypes that have high chance for resistance character to root rot disease (R. solani) with high seed yield potential. The genetic advance and heritability estimates in all cases supported the selection of some sesame genotypes to be used in next breeding programs for root rot resistance, they also showed a high seed yield potential.

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Authors and Affiliations

Omar Abdul Rahman Abdul Wahid
Mohamed Abd El-Hamid Sayid Ahemad El-Bramawy
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Abstract

Serious losses caused by root rot of sugar beet were observed in Poland in 2001 and 2002. The disease occurred in most of regions of sugar beet cropping from June to the end of growing season. Very high losses of yield were observed on many fields especially on cultivar Lolita in 2001. The aim of this work was to detect the casual agent of root rot (2001) and to compare susceptibility of sugar beet cultivars to this disease (2002–2003). The laboratory tests (2001) showed that most of destroyed roots were infected by Aphanomyces cochlioides Drechsler. This species is very well known as a pathogen of sugar beet seedling damping-off in Poland. The fungus Rhizoctonia solani Kühn was found only in 12% of rotten roots collected in south-eastern part of Poland. In field trails the highest number of infected roots and the highest infection inde xwere found for cultivar Arthur (2002–2003). That cultivar was significantly more susceptible to root rot than the rest of tested cultivars.

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Authors and Affiliations

Jacek Piszczek
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Abstract

Essential oils from four plants , i.e. geranium, rosa, lemon and mint were tested for their activity in vitro and in vivo against Rhizoctonia solani and Fusarium oxysporum f. sp. phaseoli, the cause of root rot and wilt of beans. In vitro, they were found to have an inhibitory effect against the mycelial growth of R. solani and F. oxysporum f. sp. phaseoli. Complete inhibition in fungal growth was observed at a concentration of 4% of each essential oil and Topsin M at 400 ppm as well. In greenhouse the four essential oils were tested as seed coating and/or foliar spray. Results of seed coating at a concentration of 1% clearly demonstrate a good protection of emerged bean seeds against invasion of R. solani and F. oxysporum f. sp. phaseoli compared with the fungicide treatment. A similar trend was observed in a lower extent when the essential oils were applied as bean seeds coating followed by seedlings foliar spray under field conditions. Obvious yield increase as bean green pods, in all treatments, was significantly higher than in the control.

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Authors and Affiliations

Nehal S. El-Mougy
Nadia G. El-Gamal
Mokhtar M. Abdel-Kader

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