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Abstract

This paper presents the qualitative and quantitative results of the research into potential pathogens of genus Staphylococcus in the air in the centre and in the recreational areas of Olsztyn. The qualitative examinations were conducted for one year, from October 2002 to September 2003, with one-month intervals between measurements, which were done at testing sites with the use of sedimentation and impaction methods of sampling. When the samples were taken by the sedimentation method, the average count of this type of bacteria at various sites was higher than in those taken by the impaction method. The colonies of Staphylococcus bacteria, isolated from air samples taken four times during the test period in October 2002, January, April and July 2003, were further identified down to the species, based on biochemical tests. A wider species diversity of this type of bacteria was found in the samples taken by the sedimentation method (8 species) than by the impaction method (3 species). Among the Staphylococcus bacteria isolated from air the following species dominated: S. xylosus 61% and S. len/us 15% when the samples were taken by the sedimentation method and 31 % and 62%, respectively, with the impaction method. When the samples were taken by the sedimentation method, 8% of Staphylococcus aureus and 2% Staphylococcus epidermidis were found among all the isolated bacteria (627 cfu/m3). These species were not found in the samples taken by the impaction method.
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Authors and Affiliations

Izabella Zmysłowska
Barbara Jackowska
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Abstract

The paper presents the results of studies on detection, occurrence and differentiation of the waterborne bacterial potential pathogens from Staphylococcus genus in the water of Wigry Lake watering places in north-eastern Poland. The samples of water were collected from June 1995 to September 1999 at one-month intervals, during bathing season. Eight sampling stations were selected in the most attended watering places of the lake, situated near landing stages, camping sites and holiday resorts were investigated. The watering places in the northern part of Lake Wigry (called Ploso Northern) showed the highest number of waterborne bacterial pathogens from Staphylococcus genus. The most numerous were species: Staphylocorcus capitis, Staphylococcus cohnii, Staphylococcus len/us and Staphylococcus xylosus. Pathogens from Staphylococcus aureus species identified in I 0% of water samples in the absence of faecal bacteria from Escherichia coli species (in 100 crn' of water). The Czarna Hańcza River, which brought a lot of purified sewage from Wastewater Treatment Plant in Suwałki to northern part of Wigry Lake has immense influence on the pollution of this part Wigry Lake.
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Authors and Affiliations

Ewa Korzeniewska
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Abstract

This study is aimed to investigate culturable airborne bacteria concentrations and the composition of methicillin-resistant staphylococci in eleven different locations on the basis of specific activities conducted within different parts of the European side of Istanbul. The highest bacterial levels were observed at the Bakirkoy station (1 100 CFU/m3) while the second highest levels were found at the Bahcelievler station (1 040 CFU/m3) in October; the lowest levels (10 CFU/m3) were measured at other different stations (Atakoy, Yesilkoy). Fifteen methicillin-resistant isolates [Staphylococcus hominis (n=11), S. cohnii spp. cohnii (n=2), S. sciuri (n=1), S. capitis spp. capitis (n=1)] were identified. The disc diffusion method was used to identify the antimicrobial resistance of these isolates, it was observed that the most common resistance was to penicillin (P) (n=11), doxycycline (DO) (n=4) and tetracycline (T) (n=5). None of the isolates was resistant to imipenem, amoxicillin/clavulanic acid, vancomycin (IPM, AMC, VA). However, multiple antimicrobial resistance was found to be 26.7%. The results of this study revealed the importance of isolated methicillin-resistant staphylococci in the stations with densely active human population and traffic, for public health. As a result, the importance of resting along known shorelines, where culturable airborne bacteria concentrations are much lower, and its importance for human health have been emphasized.

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Authors and Affiliations

Nüket Sivri
Arzu Funda Bağcıgil
Kemal Metiner
Dursun Zafer Şeker
Selin Orak
Sevgi Güneş Durak
Vildan Zülal Sönmez

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