We talk to Dr. Andrzej Kruszewicz, director of Warsaw Zoo, about animals being held in “captivity.”
Empathy is one of the traits that make us human. In exploring the origins of empathy disorders, however, we can learn a lot by studying animals.
How can we learn about the diet, health and parasites of animals which have long since gone extinct? One excellent, although perhaps slightly revolting way is to study pieces of fossilized excrement, known as coprolites.
The amount and composition of lipids m some Antarctic animals were studied. The material consisted of crustaceans (Euphausia superba, Paramoera sp., Orchomene sp.), tunicates (Salpa thompsoni) and vertebrates (Notothenia rossi marmorata and Hydrurga leptonyx). The author's data are discussed on the background of available literature information.
Automation in experiments carried out on animals is getting more and more important in research. Computers take over laborious and time-consuming activities like recording and analysing images of the experiment scene. The first step in an image analysis is finding and distinguishing between the observed animals and then tracking all objects during the experiment. In this paper four tracking methods are presented. Quantitative and qualitative figures of merit are applied to confront those methods. The comparison takes into consideration the level of correct object recognition during different disturbances, the speed of computation, requirements as to the frame rate and image illumination, quality of recovering from occluded situations and others.
Enterococcus hirae belongs in the Enterococcus faecium group within the genus Enterococcus. This species occurs naturally in the environment, commensally in the alimentary tracts of animals, and pathologically for example in humans with urinary infections. Some strains of E. hirae possess virulence factors, including biofilm formation. Biofilm growth protects bacteria against host de- fences; biofilm can be a source of persistent infection. Testing bacterial strains for their ability to form biofilm might therefore facilitate their treatment or prevention. This study focuses on bio- film formation by E. hirae strains derived from various animals. This kind of testing has never been done before. A total of 64 identified E. hirae from laying hens, ducks, pheasants, ostriches, rabbits, horses and a goat were tested by means of three methods; using Congo red agar, the tube method and microtiter plate agar. The majority of strains were found to form biofilm. 62.5% of strains were biofilm-forming, four categorized as highly positive (OD570 ≥1); most strains were low-grade biofilm positive (0.1 ≤ OD 570 < 1). Related to poultry, 55 E. hirae strains were tested nd found to produce biofilm; 24 strains did not form biofilm, 31 strains were biofilm-forming; 27 strains showed low-grade biofilm formation, and four strains were highly biofilm-forming. Four strains from hens and ostriches reached the highest OD570 values, more than 0.500. Rabbit-derived E. hirae strains as well as strains isolated from horses and the goat were low-grade bio- film-forming. Microtiter plate assay proved to be the best tool for testing the in vitro biofilm for- mation capacity of E. hirae strains from different species of animals.
The substrates to biogas production in anaerobic digestion, except plant materials, can also be animal feces and manure. It should be highlighted that Poland is one of leaders in the European Union in animal breeding. However, there is no precise data in the literature on the potential of biogas production from animal feces in this country. The aim of the paper was to analyze the biogas production potential from manure in Poland. The aim of work included anaerobic digestion research following materials: cow manure, pig manure, poultry manure and sheep manure. In the next step, based on the obtained results of the biogas yield, energy potential calculations were made. The methane yield for the investigated feedstock materials in the batch culture technology was performed following the internal procedures developed based on the adapted standards, i.e. DIN 38 414-S8 and VDI 4630. Animal wastes were obtained from the Agricultural Experimental Stations of Poznan University of Life Sciences (Poznan, Poland). On a base of achieved results it was concluded that tested substrates have a high energy potential (approx. 28.52 GWh of electricity). The largest potential for electricity production was found in chicken manure (about 13.86 GWh) and cow manure (about 12.35 GWh). It was also shown which regions of Poland have the best chance for development of agriculture biogas plants (Wielkopolskie and Mazowieckie voivodships) and where the potential is the least (Lubuskie and Opolskie voivodeships).
This study is aimed at measuring the effect of pig, cow, horse and poultry manures on the degradation of
selected Polycyclic Aromatics Hydrocarbons present in oil sludge. Four kilograms of soil amended with 1.2 kg of oil
sludge was mixed with wood chips in a ratio of 1:2 (w:v) soil mixture: wood chips. The mixture was divided into fi ve parts
and four parts were separately mixed with pig, cow, horse or poultry manures in a ratio of 2:1 (w:w) and the fi fth portion
was used as the control with no manure added. All experiments were incubated for 10 months at room temperature.
Compost piles were turned weekly for aeration and moisture level was maintained by adding deionised water enough
to prevent the compost from getting dry. Moisture level, pH, temperature, CO2
evolution and oxygen consumption
were measured monthly and the ash content of the compost at the end of experimentation. Highest temperature reached
was 27.5°C in all compost heaps, pH ranged from 5.5 to 7.8 and CO2
evolution was highest in poultry manure at
18.78 μg/dwt/day. Microbial growth and activities were enhanced as indicated by increase in temperature, moisture
level, pH value and respiration rate in all the compost piles. Bacteria capable of utilizing PAHs were isolated, purifi ed
and characterized by molecular techniques using polymerase chain reaction with specifi c universal primers and the
amplicons were sequenced. Bacteria identifi ed were Bacillus, Arthrobacter and Staphylococcus species. Percentage
reduction in PAHs was measured using automated soxhlet extractor with Dichloromethane and gas chromatography/mass
spectrometry. Results from PAH concentration measurements showed reduction of between 77% and 99%. Co-
-composting of contaminated soil with animal manures enhanced the reduction in PAHs.
Compounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading capabilities from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identification using polymerase chain reaction (PCR) of the 16S rRNA gene with specific primers (universal forward 16S-P1 PCR and reverse 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades; Firmicutes, Proteobacteria and Actinobacteria. These bacteria identified were closely related to the genera Bacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus. The results showed that Bacillus species were predominant in all composts. Based on the results of the degradation of the PAHs in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAHs of different molecular weights in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation.
Layers of silver particles are used in the studies on pathophysiology and treatment of diseases, both in pre-clinical and clinical conditions. Silver layers can be formed using different techniques and on different substrates. Deposition by magnetron sputtering on glass beads was used in this study. Silver absorption by the body was estimated by calculating the difference in thickness of the silver nanolayer deposited on a bead and measured before and after application of the bead in an animal model of gastrointestinal inflammation. Recommendations for the minimal thickness of silver nanolayer deposited on glass beads were worked out for further studies.
Canine distemper virus (CDV) infects wild and domestic Canidae worldwide. The hemag- glutinin (H) gene has the highest genetic variation in the genome of this virus. Thus, the H gene is commonly used for lineage identification and genetic analyses. In order to study the genetic characteristics and pathogenicity of CDV strains prevalent in China, 132 samples were collected from domestic dogs with suspected CDV infection, 58 samples were confirmed to be positive, and the H gene was successfully amplified from 15 samples. The epidemic strain was identified as type Asia-1 and the novel mutations, A51T, V58I, R179K and D262N, were detected in this strain. Isolated strains, BJ16B53, BJ16B14, and BJ17B8, were used for an animal infection experiment in raccoon dogs. BJ16B53 and BJ16B14 were found to cause clinical symptoms, death, and exten- sive lesions in various organs. These results are expected to facilitate the development of effective strategies to monitor and control CDV infection in China.
The aim of this paper is to analyse various animal-specifi c complex lexical units together with patterns that can be held responsible for their underlying conceptual structure. Many examples of the data investigated in the paper seem to represent compounds as they are traditionally understood in the literature of the subject (see, among others, Bauer 2003; Katamba and Stonham 2006; Lieber and Štekauer 2009; Fàbregas and Scalise 2012; Bauer et al. 2013); however, others do not meet the basic criteria for compoundhood as postulated by, for example, Altakhaineh (2016). In my research I use the term animal-specifi c complex lexical units with reference to all animal-related composite expressions being the result of the working of metaphor-metonymy interaction.
The pace of climate change observed since the beginning of the industrial era has prompted scientists to seriously consider whether human activity is to blame for global warming. On the geological timescale, however, climate change is certainly nothing new or exceptional – as is clear when one looks at the record of plant and animal fossils.
Animals kept outside their natural environment often suffer from boredom. They don’t hunt or have a chance to conduct their mating rituals, and their natural tendency for physical activity is limited by space. These deficiencies affect their psychological well-being. But when it comes to dogs, we can help them by exploiting their excellent sense of smell.
Tribal fragments of the Cumans, a people of the Eurasian steppe region, appeared in the medieval kingdom of Hungary in the early 13th century, on the eve of the Mongol Invasion. Many of them permanently settled in the Great Hungarian Plain, and their community had to undergo profound transformations both in terms of social and economic strategies. Mobile pastoralism, often associated with the Cuman communities of the steppe, was definitely impossible in their new homeland. However, animal husbandry remained the most important economic activity in this part of the Carpathian Basin in the centuries after the Cumans’ arrival. This paper provides a case study on the region called Greater Cumania in the Great Hungarian Plain, and especially on one Cuman village, Orgondaszentmiklós, where 14th–16th-century habitation layers were brought to light. Archaeological and written evidence for animal husbandry is analyzed in order to establish patterns of integration or specialization in terms of animal herding. The results show that although some preferences that may have been rooted in steppe tradition were retained, the main factor in economic orientation was the position in the settlement network and the connection to markets. Swine keeping, a tradition virtually non-existent in the steppe area, was adapted relatively quickly as a response to available natural resources (marshlands) in the area. It seems, on the other hand, that horses preserved their high social value and their flesh was also consumed.
One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.
The article tackles issues of the pedagogy of place, with a special emphasis on the urban context. On the basis of fundamental theses asserting that a city is a text (Vladimir Toporov) and that places are pedagogical (Maria Mendel) – the author concentrates on symbols. Definition of this concept, as well as de3 nitions of its particular exemplifications, create a theoretical basis for further practical considerations. Presenting methodology of a research project Reading the City (Czytanie miasta) conducted in the years 2015–2016, the author creates new research challenges for pedagogues. Presentation of wide variety of city symbolism, encompassing both cultural manifestations (towers, bridges) and a realm of nature (mountains, river), leads to proposed educational applications. Descriptions of realized animations based on research form a summary of this article.
Introduction: Effective and safe anesthesia for rodents has long been a leading concern among biomedical researchers. Intraperitoneal injection constitutes an alternative to inhalant anesthesia.
Purpose: The aim of this study was to identify a safe, reliable, and effective anesthesia and postoperative analgesia protocol for laboratory rats exposed to painful procedures.
Material and methods: Twenty-seven female Wistar rats in an ongoing study that required surgery were randomized into groups for three different intraperitoneal anesthesia protocols and three different analgesia regimens. The anesthesia groups were (1) medetomidine + ketamine (MK), (2) ketamine + xylacine (KX), and (3) fentanyl + medetomidine (FM). Three analgesia groups were equally distributed among the anesthesia groups: (1) local mepivacaine + oral ibuprofen (MI), (2) oral tramadol + oral ibuprofen (TI), and (3) local tramadol + oral tramadol + oral ibuprofen (TTI). A core was assigned to measure anesthesia (0-3) and analgesia (0-2) effectiveness; the lower the score, the more effective the treatment.
Results: The mean MK score was 0.44 versus 2.00 for FM and 2.33 for KX. Mean score for analgesia on the first postoperative day was TTI (4.66) TI (9.13), and MI (10.14). Mean score 48 hours after surgery was TTI (3.4), TI (6.71), and MI (9.5). These differences were statistically significant.
Conclusion: MK was shown to be a reliable, safe, and effective method of anesthesia. The TTI analgesia regimen is strongly recommended in light of these results.
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