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Abstract

The aim of this study was to evaluate the antioxidant effect of selenium in Pisum sativum L. plants pre-treated with sodium selenite or sodium selenate at a concentration of 10 and 20 μM, and then colonized by pea aphid Acyrthosiphon pisum (Harris). It has been hypothesized that selenium at low concentrations alleviates oxidative stress caused by aphid feeding on pea leaves. The study focused on the generation of reactive oxygen species (superoxide anion, hydrogen peroxide and hydroxyl radical), the activities of the antioxidant enzymes (superoxide dismutase and ascorbate peroxidase) scavenging the reactive oxygen species levels, as well as on total antioxidant activity in pea leaves. Selenium in pea leaves exposed to aphid feeding affected changes in the levels of reactive oxygen species, the activity of studied antioxidant enzymes, and the total antioxidant capacity. Effects depended on the form and concentration of selenium, as well as on the time after the colonization of pea plants by aphids. Obtained results showed beneficial effects of selenium in alleviating oxidative stress in pea leaves caused by aphid feeding.
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Authors and Affiliations

Sabina Łukaszewicz
1
Barbara Politycka
1
Beata Borowiak-Sobkowiak
2

  1. Department of Plant Physiology, Poznań University of Life Sciences, Poznań, Poland
  2. Department of Entomology and Environmental Protection, Poznań University of Life Sciences, Poznań, Poland
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Abstract

The aim of this study was to evaluate the antioxidative/oxidative status of spermatozoa and prostatic fluid in dogs with benign prostatic hyperplasia (BPH) by the determination of total antioxidant capacity and protein peroxidation markers. Study was conducted on 40 intact dogs of various breeds. The dogs were assigned to two groups: BPH group (n=20) and non-affected group (n=20). The second and third fractions of the ejaculate were collected separately by digital manipulation. Total antioxidant capacity (TAC) and the concentrations of SH-groups in sperm and prostatic fluid were determined spectrophotometrically, the concentrations of bityrosine and formylkynurenine were determined using spectrofluorimetric methods. The mean values of TAC in spermatozoa and prostatic fluid were significantly lower (p<0.05), whereas the mean contents of biotyrosine and formylkinurenine were significantly higher (p<0.05) in BPH dogs compared to control dogs. There was no statistically significant difference in the content of SH group between dogs with BPH and control dogs (p>0.05). In conclusion, the results indicate that BPH in dogs is associated with reduced total antioxidant capacity and increased protein oxidation in the prostatic fluid and spermatozoa, and suggest the importance of oxidative stress in the pathogenesis of this condition. The potential role of antioxidants in the prevention and therapy of canine BPH requires further studies.
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Authors and Affiliations

A. Domosławska
1
S. Zduńczyk
1
A. Bielecka
2
M. Kankofer
2

  1. Department of Animal Reproduction with Clinic, University of Warmia and Mazury, 10-719 Olsztyn, Oczapowskiego 14, Poland
  2. Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences, 20-033 Lublin, Akademicka 12, Lublin, Poland

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