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Number of results: 7
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Abstract

This study was executed to investigate the potential of agar-agar, a nontoxic and non-degradable

gelling agent, as a promising coating agent to improve and protect banana fruit

against fungal postharvest diseases i.e., crown, finger, neck and flower end rots which are

caused by fungal isolates of Colletotrichum musae and Fusarium moniliforme. Coated-ba-nana

fruit samples with different concentrations of agar-agar suspension particularly at

2.0 g · l−1 exhibited a significant reduction in incidence and severity of postharvest diseases

compared to untreated fruit. Banana fruits dipped in agar suspension at 2.0 g · l−1 for 5, 10

and 15 min showed significant reduction in disease incidence and severity. Moreover,

application of agar suspension as a coating agent at 2.0 g · l−1 significantly decreased

weight loss (%), firmness loss (%), and soluble solid concentration of banana fruit for

15 days at 25 ± 2°C. Scanning electron microscopy observation confirmed that the fruit

coated with agar colloid at 2.0 g · l−1 had significantly fewer cracks and showed smoother

surfaces than untreated fruit. This explains the quality improvement in agar-coated fruit

compared to uncoated fruit. Overall, agar colloid, a safe coating agent, could be used to

protect banana fruit against postharvest rot diseases and extend fruit storage life during

ripening and storage.

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Authors and Affiliations

El Sayed Hussein Ziedan
Hassan Mohamed El Zahaby
Hanafey Farouk Maswada
El Hassan Abd El Rafh Zoeir
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Abstract

Drought is regarded as one of the environmental constraints threatening agriculture worldwide. Melatonin is a pleiotropic molecule prevalent in plants capable of promoting plant endogenous resilience to many environmental challenges including drought. Banana is an important staple food consumed in developing countries especially in Africa. In this research, we studied the role of melatonin in the growth of bananas subjected to drought under the Egyptian semi-arid conditions. To achieve this objective, a field experiment on banana (Musa spp., cv. Williams) mother plants and first ratoon was conducted on a private farm for two seasons - 2019 and 2020. Three irrigation treatments, 100, 90 and 80% irrigation water requirements (IWR) were used in conjunction with four concentrations of melatonin as a foliar spray (0 μmol, 40 μmol, 60 μmol, and 80 μmol) to determine the effect of both treatments on banana plant performance under drought. The results showed that there was a substantial difference between treatments, with the foliar application of melatonin at 80 μmol concentration improving most of the yield attributes, relative water content, total chlorophyll and proline with water deficit. However, the foliar application of the molecule lowered the biochemical characteristics mostly at 80% IWR under the Egyptian semi-arid conditions. Overall, there was a concentration-dependent response with regards to IWR for the two seasons 2019 and 2020.
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Authors and Affiliations

Islam F. Hassan
1
ORCID: ORCID
Maybelle S. Gaballah
1
ORCID: ORCID
Chukwuma C. Ogbaga
2
ORCID: ORCID
Soha A. Murad
3
ORCID: ORCID
Adam Brysiewicz
4
ORCID: ORCID
Basem M.M. Bakr
5
ORCID: ORCID
Amany Mira
6
ORCID: ORCID
Shamel M. Alam-Eldein
6
ORCID: ORCID

  1. National Research Centre (NRC), Agriculture and Biology Research Institute, Water Relations and Field Irrigation Department, Postal Code, 12622, 33 El Buhouth St, Dokki, Giza, Egypt
  2. Nile University of Nigeria, Department of Microbiology and Biotechnology, Abuja, Nigeria
  3. National Research Centre (NRC), Agriculture and Biology Research Institute Plant BioChemistry Department, Dokki, Giza, Egypt
  4. Institute of Technology and Life Sciences – National Research Institute, Falenty, Poland
  5. National Research Centre (NRC), Agriculture and Biology Research Institute, Pomology Department, Dokki, Giza, Egypt
  6. Tanta University, Faculty of Agriculture, Department of Horticulture, Tanta, Egypt
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Abstract

Electrolyzed fertilizer solution (EFS) was produced by passing an irrigation solution through an electrolization chamber in order to suppress fungal disease caused by Fusarium oxysporum f. sp. cubense race 4 (Foc4) infecting banana plantlets. In the laboratory, EFS was prepared by electrolyzing solutions containing different amounts of potassium chloride and potassium nitrate. The results indicated a significant reduction in the conidial densities of Foc4 which was from 10 6 spores · ml –1 to a maximum of 10 1.3 spores · ml –1 and depended on the concentration of components in the input flow. Eventually the EFS produced from the lowest one was chosen to treat banana plantlets. Greenhouse experiments gave contradictory results of inoculated plantlets irrigated with or without EFS. The untreated banana plantlets virtually showed symptoms of infection such as discoloration of cross-cut corms, rapid wilting and dying within 60 days, while the treated ones kept their shapes and grew normally. The drastic fall in the microbial population in the rhizosphere of treated plants confirmed the activity of oxidation agents which is the major mechanism of disease suppression. The results suggest that further studies of EFS in the field as a potential technique in fighting Panama wilt in the banana industry are necessary.
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Authors and Affiliations

Vu Anh Nguyen
1
ORCID: ORCID
Ha Van Nguyen
2
Phai Duy Do
3
Hung Ngoc Tran
4

  1. Department of Environmental Physico-Chemistry, Institute of Environmental Technology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam
  2. Department of Technology Application and Transfer, Institute of Environmental Technology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam
  3. Central Analytical Laboratory, Soils and Fertilizers Research Institute, Hanoi, Viet Nam
  4. Department of Biotechnology, Fruit and Vegetable Research Institute, Hanoi, Viet Nam
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Abstract

Banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis ( Rsc), is a major threat to banana production in Southeast Asia. This study aimed to assess the resistance of cultivated and wild banana accessions to Rsc and investigate the expression of pathogenesis- related (PR) protein genes, namely PR3 and PR10, in disease-resistant bananas. Bacterial isolates were isolated from infected bananas in Yala Province, Thailand, and their pathogenicity and phylotype were confirmed, along with Rsc-specific PCR. Rsc-resistance banana screening was conducted on 16 banana accessions, including cultivated and wild types, using representative Rsc isolates. ‘Khai Kasetsart 2’ exhibited resistance (R), followed by ‘Raksa’ with moderate resistance (MR). The expression of PR3 and PR10 genes was analyzed in the resistant ‘Khai Kasetsart 2’ and susceptible ‘Hin’ bananas, revealing distinct expression patterns. PR3 showed rapid upregulation on day 1 after inoculation (DAI), while PR10 exhibited sustained upregulation from 1 to 7 DAI in the resistant cultivar. These findings indicate the involvement of PR proteins in the defense response against Rsc and hold promise for future breeding and disease management strategies in bananas.
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Authors and Affiliations

Jariya Nitayaros
1
Thanwanit Thanyasiriwat
1
Aphidech Sangdee
2
Ladawan Rattanapolsan
1
Ratri Boonruangrod
3
Praphat Kawicha
1

  1. Plant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand
  2. Department of Biology, Mahasarakham University, Faculty of Science, Maha Sarakham, Thailand
  3. Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand
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Abstract

Influence of Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyder and Hansen on 2,4-diacetylphloroglucinol (DAPG) production in the rhizosphere of banana cultivar Rasthali by Pseudomonas fluorescens was investigated. The purified extracts of Pfm strain of P. fluorescens isolated from banana rhizosphere inhibited the growth and spore germination of F. oxysporum f. sp. cubense under laboratory conditions. DAPG extracted from the cultures of the strain was observed as distinct spots in thin layer chromatographic plates at Rf value of 0.88. The extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense eluted at retention time ranges from 20.00 min to 21.30 min. The quantity of DPAG production was less in the extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense as compared to P. fluorescens alone inoculated soil. The talc formulation of Pfm strain also reduced vascular discolouration due to the pathogen in banana plants when inoculated at 15 g/plant.

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Authors and Affiliations

Thangavelu Saravanan
Mari Muthusamy
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Abstract

This article presents a quality analysis of convectively dried fruits and vegetables with preliminary osmotic dehydration. Tests were carried out on banana fruit and red beetroot samples. Hypertonic solutions of fructose for the banana and those of sucrose for the red beetroot were used, each one at three different concentrations. After osmotic dewatering treatment conducted at different time intervals and after osmotic dehydration the samples were dried convectively until an equilibrium with the surroundings was attained. Osmotic dehydration and convective drying curves were determined. The values of Solids Gain (SG), Water Loss (WL) and Weight Reduction (WR) were measured and changes in the samples’ colour and shape after convective drying with and without osmotic pretreatment were assessed.

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Authors and Affiliations

Stefan J. Kowalski
Joanna M. Łechtańska
Justyna Szadzińska
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Abstract

Arsenic is one of the most harmful pollutants in groundwater. In this paper, the Nepali bio sand filter (BSF) was modi-fied with different bio-adsorbents, and proved to be an efficient method for arsenic removal from groundwater. Three dif-ferent bio-adsorbents were used to modify the Nepali BSF. Iron nails and biochar BSF, ~96% and ~93% arsenic removal was achieved, within the range of WHO guidelines. In iron nails, BSF and biochar BSF ~15 dm3∙h–1 arsenic content water was treated. In the other two BSFs, rice-husk and banana peel were used, the arsenic removal efficiency was ~83% of both BSFs. Furthermore, the efficiency of rice-husk and banana peel BSFs can be increased by increasing the surface area of the adsorbent or by reducing the flow rate.

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Authors and Affiliations

Ghulam S. Keerio
1
Hareef A. Keerio
2
ORCID: ORCID
Khalil A. Ibuphoto
3
Mahmood Laghari
1
Sallahuddin Panhwar
4
Mashooque A. Talpur
5

  1. Sindh Agriculture University, Department of Energy and Environment, Tandojam, Pakistan
  2. Hanyang University, Department of Civil and Environmental Engineering, Seoul, South Korea
  3. Sindh Agriculture University, Department of Farm Structures, Tandojam, Pakistan
  4. Mehran University of Engineering and Technology, US-Pakistan Centers for Advanced Studies in Water, Jamshoro, Pakistan
  5. Sindh Agriculture University, Department of Irrigation and Drainage, Tandojam, Pakistan

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