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First identification of tomato leaf curl Palampur virus in Oman: detection and characterization

Muhammad Shafiq Shahid Abdullah M. Al-Sadi

Journal of Plant Protection Research | 2022 | vol. 62 | No 3 | 295-301 | DOI: 10.24425/jppr.2022.142136

Keywords bipartite begomovirus diversity papaya ToLCPlV

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Abstract

The complete genome sequence of tomato leaf curl Palampur virus (ToLCPlV), that infects a papaya ( Carica papaya) plant, was determined. The virus genome was composed of 2,756 and 2,719 nucleotides (nt) in length, encoding all proteins required for replication, encapsidation and movement with the genome features typical of a bipartite begomovirus. Pairwise identity, derived using the Sequence Demarcation Tool (SDT), identified that the virus DNA A and DNA B shared maximum sequence identity 98–99% corresponding to the DNA A of ToLCPlV ([IR: Jir-T65X:08] JF501720) and 96–98% to the cognate partner DNA B of ToLCPlV ([IR: Jir1:T55P:07] FJ660423), respectively. The evolutionary relation using phylogenetic dendrograms of DNA A and DNA B genome components were clustered with ToLCPlV genomes of DNA A and DNA B of Iranian isolates. This study provides the first evidence of a bipartite ToLCPlV infecting papaya in the Sultanate of Oman and also indicates the requirement for more surveillance of this virus in Oman, as ToLCPlV is a major threat to tomato and other vegetable crops in South Asia (India and Pakistan) and in Iran.

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Authors and Affiliations

Muhammad Shafiq Shahid

Abdullah M. Al-Sadi

Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod, Oman

First record of a novel begomovirus and satellites associated with leaf curl disease of passion fruit from India

Venkataravanappa Venkataravanappa Lakshminarayana Reddy Cheegatagere Narasimha Reddy Shridhar Hiremath Bommanahalli Munivenkategowda Muralidhara Suryanarayana Vishweswarasastry Virendra K. Baranwal Krishna Reddy Manem

Journal of Plant Protection Research | 2022 | vol. 62 | No 1 | 78-92 | DOI: 10.24425/jppr.2022.140303

Keywords begomovirus passion fruit phylogenetic analysis recombination sequence demarcation tool (SDT)

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Abstract

Passion fruit is an important fruit crop grown in parts of southern and north-eastern states of India. Leaf curl symptoms typical to begomovirus infection were observed on passion fruit plants at three locations of Madikeri District, Karnataka State, India. The disease incidence ranged from 10–20% in all the locations. In order to determine if the begomovirus was associated with leaf curl disease of passion fruit, 20 infected samples collected from different locations were subjected to PCR analysis using primers specific to begomovirus. This resulted in an expected PCR product of ~1.2 kb. Sequence analysis of these products revealed that they have more than 98% similarity among them and have similarity with other begomoviruses. Complete genome sequencing of begomovirus associated with one sample (PF1 collected from CHES, Madikeri) was done using RCA. Further, sequencing of betasatellite and alphasatellite was done after PCR amplification using specific primers. Complete DNA-A sequence of PF-isolate with other begomoviruses revealed that it shared nucleotide (nt) identity of 87.8 to 88.8% with Ageratum enation virus. This indicated the association of a novel begomovirus with leaf curl disease of passion fruit in India, for which we propose the name, Passion fruit leaf curl virus (PFLCuV) [IN-Kar-18]. PFLCuV associated betasatellite shared 98.3% sequence identity with Tomato leaf curl Bangladesh betasatellite, while alphasatellite had 95.7% sequence identity with Cotton leaf curl Multan alphasatellite. Recombinant analysis indicated a major component of PFLCuV DNA-A may have originated from a recombination of earlier reported begomoviruses. Recombination as well as GC plot analysis showed that the recombination occurred in the genome regions having low GC content regions of PFLCuV. However, there is no evidence of recombination in alphasatellite and betasatellite associated with leaf curl disease of passion fruit. This is the first record of a novel begomovirus and satellites associated with leaf curl disease of passion fruit from India.

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Authors and Affiliations

Venkataravanappa Venkataravanappa

Lakshminarayana Reddy Cheegatagere Narasimha Reddy

Shridhar Hiremath

Bommanahalli Munivenkategowda Muralidhara

Suryanarayana Vishweswarasastry

Virendra K. Baranwal

Krishna Reddy Manem

Central Horticultural Experimental Station, Indian Council of Agricultural Research – Indian Institute of Horticultural Research, Chettalli, Madikeri, Karnataka, India
Department of Plant Pathology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore, Karnataka, India
Indian Council of Agricultural Research – Directorate of Cashew Research, Puttur, Karnataka, India
Department of Forest Biology and Tree Improvement, University of Agricultural Science, Dharwad, Sirsi, Karnataka, India
Division of Plant Pathology, Indian Council of Agricultural Research – Indian Agricultural Research Institute, New Delhi, India
Indian Council of Agricultural Research – Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore, Karnataka, India

Chili ( Capsicum annuum L.) genotypes resistant to Pepper yellow leaf curl Thailand virus (PepYLCTHV)

Patcharaporn Suwor Tawatchai Masirayanan Hathairat Khingkumpungk Wen Shi Tsai Kanjana Saetiew Suchila Techawongstien Sanjeet Kumar Somsak Kramchote

Journal of Plant Protection Research | 2021 | vol. 61 | No 4 | 377-383 | DOI: 10.24425/jppr.2021.139246

Keywords artificial inoculation begomovirus genetic resistance graft inoculation purelines

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Abstract

Pepper yellow leaf curl Thailand virus (PepYLCTHV) causes leaf curl disease in chili production regions of the tropics and subtropics. Information on PepYLCTHV disease severity and resistance in chili pepper is still limited in Thailand. This study reports PepYLCTHV disease severity through graft inoculation and selection of single resistant plants for use in a chili breeding program. Twenty-one chili genotypes consisting of the local cultivar (5) collected from Thailand, breeding lines (9) developed at Khon Kaen University (KKU), Thailand and improved lines (7) obtained from the World Vegetable Center, Taiwan were used in this study. Forty-five-day-old seedlings of all the genotypes were graft inoculated with PepYLCTHV in a randomized complete block design (RCBD) with three replications and 10 plants per replication and kept in a plastic net house. Disease symptoms were scored at 20, 27, 34, 41 48, and 55 days after graft/inoculation (DAI). Disease severity was visually recorded using 0−5 scores. Results showed that the disease severity of 21 chili genotypes significantly differed at 48 days after grafting. High resistance and stability were shown by 9853-123 genotypes. Two genotypes, PSP11-7 and PSP11-10-1, showed resistant reaction with disease severity scores of 1.9 and 1.8, respectively. However, among 21 chili genotypes or 630 grafted plants, 302 plants were successfully grafted inoculated plants. Therefore, from the results of this work, highly resistant plants (69 single plants) can be selected, selfed and advanced for breeding.

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Bibliography

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Authors and Affiliations

Patcharaporn Suwor

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Tawatchai Masirayanan

Hathairat Khingkumpungk

Wen Shi Tsai

Kanjana Saetiew

Suchila Techawongstien

Sanjeet Kumar

Somsak Kramchote

Plant Production of Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand
Department of Plant Medicine, College of Agriculture, National Chiayi University, Chiayi, Taiwan
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
Pepper Breeding Section, Plant Geneticist and Breeder (Independent), India

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First identification of tomato leaf curl Palampur virus in Oman: detection and characterization

Abstract

Authors and Affiliations

First record of a novel begomovirus and satellites associated with leaf curl disease of passion fruit from India

Abstract

Authors and Affiliations

Chili ( Capsicum annuum L.) genotypes resistant to Pepper yellow leaf curl Thailand virus (PepYLCTHV)

Abstract

Bibliography

Authors and Affiliations