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Abstract

The paper reports results of investigations concerning the influence of saponin extract of quillaja on the food choice and development of Colorado potato beetle. Choice tests with the use of potato leaf discs treated and untreated with tested saponins’ extract were made. The influence of the presence of saponins on the food choice and intensity of insect feeding were observed. It was stated that saponins significantly restrain feeding and disturb development of this pest.

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Authors and Affiliations

Danuta Waligóra
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Abstract

The present study investigated the chemical coding of neurons and nerve fibres in local laryngeal ganglia in pigs (n=5) using double-labelling immunohistochemistry. Virtually all the neurons were cholinergic in nature (ChAT- or VAChT-positive). Only very solitary, small nerve cells (presumably representing interneurons) stained intensely for adrenergic marker, DβH. Many neurons also contained immunoreactivity for NOS (91%), VIP (62.7%), NPY (24.7%), galanin (10%), SP (1.3%) and CGRP (5.3%). No neurons expressing somatostatin or Leu-enkephalin were observed. Nearly all the neuronal somata were densely supplied with varicose cholinergic nerve terminals, which presumably represented preganglionic axons, and some of them were also closely apposed with CGRP- and/or SP-positive varicose nerve endings, which were putative collaterals of extrinsic primary sensory fibres. In conclusion, this study has revealed that intrinsic neurons in the porcine larynx, like in many other mammalian species studied, should be classified as parasympathetic cholinergic neurons expressing biologically active substances, predominantly NOS and VIP. Furthermore, they are likely to receive inputs from not only preganglionic neurons but also primary sensory nerve cells. Finally, it appears that the information on the occurrence of the local laryngeal ganglia should be regularly included in textbooks dealing with the cranial portion of the parasympathetic nervous system in mammals.
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Authors and Affiliations

W. Sienkiewicz
1
M. Klimczuk
1
M. Gulbinowicz-Gowkielewicz
2
E. Lepiarczyk
3
J. Kaleczyc
1

  1. Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
  2. Department of Otolaryngology and Laryngological Oncology, Voivodal Specialistic Hospital, Żołnierska 18, 10-561 Olsztyn, Poland
  3. Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082, Olsztyn, Poland
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Abstract

The biofiltration process in the biologically activated carbon filters (BAC) is one of advanced methods of water treatment. It enables efficient elimination of dissolved organic matter and some inorganic pollutants. The production of high-quality drinking water requires an appropriate method of filter work control based on biofilm growth assessment. The first aim of the study was to assess the microbial development in beds of two BAC filters with the use of various methods. The second aim was to compare the obtained results and indicate the method which could support filter operators during routine control of biofiltration process. The study was carried out in a pilot scale on models of BAC filters during two filter runs. The analysis of Microorganisms was performed in water samples collected from different depths of the filter beds with the use of culture method (HPC), metabolica ctivity assay (with the FDA), epifluorescence microscopy – total cell count method (TCC) and biochemical method (system Vitek 2 Compact). No statistical correlation between HPC and metabolic activity assay was noted. Total bacteria number determined with the use of TCC was approx. 100–900 times higher than in the HPC method. The biochemical tests revealed the presence of several Gram-negative species. The comparison of the applied methods shows that microbial activity assay is the most useful, fast and low-cost method which may be applied additionally to the HPC method at standard water treatment plant laboratory.
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Authors and Affiliations

Dorota Holc
1
ORCID: ORCID
Beata Mądrecka-Witkowska
1
ORCID: ORCID
Małgorzata Komorowska-Kaufman
1
ORCID: ORCID
Elżbieta Szeląg-Wasielewska
2
Alina Pruss
1
ORCID: ORCID
Zefiryn Cybulski
3

  1. Poznan University of Technology, Institute of Environmental Engineering and Building Installations, Poland
  2. Adam Mickiewicz University in Poznań, Faculty of Biology, Department of Water Protection, Poland
  3. Greater Poland Cancer Center, Microbiology Laboratory, Poland
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Abstract

Filtration through biologically active carbon (BAC) filters is an effective method of organic matter removal during drinking water treatment. In this study, the microbial community in the initial period of filters’ operation, as well as its role in the organic matter removal were investigated. Research was carried out in a pilot scale on two BAC filters (Filter 1 and Filter 2) which were distinguished by the type of inflowing water. It was observed that the number of heterotrophic plate count bacteria and total microbial activity were significantly higher in water samples collected from Filter 2, which received an additional load of organic matter and microorganisms. Despite the differences in the values of chemical and microbiological parameters of inflowing water, the composition of the microbiome in both filters was similar. The predominant taxon was a bacterium related to Spongiibacter sp. (Gammaproteobacteria) (>50% of relative abundance). In both filters, the efficiency of organic matter removal was at the same level, and the composition and relative frequency of predicted functional pathways related to metabolism determined using PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States Software) at level 3 of KEGG (Kyoto Encyclopedia of Genes and Genomes) Orthology – were also similar. The study demonstrated that a 40-day period of filter operation after filling with virgin granular activated carbon, was sufficient to initiate biofilm development. It was proved, that during the initial stage of filter operation, microorganisms capable of biodegradation of various organic compounds, including xenobiotics like nitrotoluene, colonized the filters
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Authors and Affiliations

Beata Mądrecka-Witkowska
1
ORCID: ORCID
Małgorzata Komorowska-Kaufman
1
ORCID: ORCID
Alina Pruss
1
ORCID: ORCID
Dorota Holc
1
ORCID: ORCID
Artur Trzebny
2
ORCID: ORCID
Miroslawa Dabert
2
ORCID: ORCID

  1. Poznan University of Technology, Institute of Environmental Engineering and Building Installations, Poznań, Poland
  2. Adam Mickiewicz University in Poznań, Faculty of Biology, Poznań, Poland
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Abstract

The paper discusses the impact of pesticides on the biological activity of soils, as well as an environmental assessment of the state of light chestnut soils by the Kazakh Research Institute of Agriculture and Crop Production with the aim to es-tablish diagnostic indicators that reduce biological activity. The study covers physical, chemical and biological properties of soils under crops of winter wheat in the light chestnut soil in the South-East of the Republic of Kazakhstan. The content of pesticides in soil samples was determined using the gas chromatography mass-spectrometric method. The paper shows results of the chromatographic analysis of soil samples regarding the content of pesticides. The study of changes of light chestnut soil biological activity was conducted to determine their relative resistance to pesticide contamination. Data ob-tained revealed the degree of light chestnut soil resistance to pesticide contamination. The study also identified species of soil invertebrates, as well as soil enzymes that should be used as bioindicators for the monitoring of the contamination with pesticides. Results obtained expand knowledge about changes in the biological activity of light chestnut soils due to pesti-cide contamination in the ecosystems of South-East Kazakhstan. In contrast to abundance indicators, the results suggest that the species composition of soil organisms can be used as a criterion for a qualitative assessment of the soil exposure to pesticides.
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Authors and Affiliations

Zulfiya Tukenova
1
ORCID: ORCID
Mustafa Mustafayev
2
ORCID: ORCID
Mereke Alimzhanova
3
ORCID: ORCID
Turar Akylbekova
4
ORCID: ORCID
Kazhybek Ashimuly
5
ORCID: ORCID

  1. Al-Farabi Kazakh National University, Faculty of Geography and Environmental Sciences, Department of UNESCO in Sustainable Development, Almaty, Republic of Kazakhstan
  2. Azerbaijan National Academy of Science, Institute of Soil Science and Agrochemistry, 5, M. Rahim str., Baku, AZ10073, Azerbaijan Republic
  3. Al-Farabi Kazakh National University, Faculty of Thermal Physics and Technical Physics, Department of Physics and Technology, Almaty, Republic of Kazakhstan
  4. Abai Kazakh National Pedagogical University, Departments of Chemistry, Institute of Natural Sciences and Geography, Almaty, Republic of Kazakhstan
  5. Scientific Production Center of Microbiology and Virology, Almaty, Kazakhstan
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Abstract

This review presents the results of multi-year efforts for the development and integral investigation of the collection of Deschampsia antarctica É. Desv. plants originated from the maritime Antarctic. To create the collection, we have optimized the procedures and conditions for germination of seeds and generation of aseptic plants in vitro together with their subsequent clonal propagation, long-term in vitro culture, and acclimatization of plants to pot culture, and finally produced fertile plants able to set viable seeds. The collection of plants cultivated in vitro and in pots is consisted of clones of 22 different genotypes originated from eight island populations from the Argentine Islands – Kyiv Peninsula region. Some of these clones have been successfully cultured for more than ten years and were found to be genetically stable over this period. The collection was shown to be heterogeneous in a number of variables. The morphological and anatomical characteristics of the plants, as well as the data of karyological, molecular cytogenetic, molecular genetics, and biochemical analysis are presented. The created collection of D. antarctica plants can provide a valuable plant material with well-studied characteristics grown under controlled conditions to perform experimental research on the mechanisms for plant adaptation and resistance to various abiotic factors of Antarctic environment. Furthermore, development of the collection and its comprehensive characterization form the basis for further biotechnological developments focused on the use of this Antarctic plant as a source of biologically active substances, which can potentially be used for therapeutic and prophylactic purposes.
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Authors and Affiliations

Viktor Kunakh
1
ORCID: ORCID
Maryana Twardovska
1
ORCID: ORCID
Igor Andreev
1
ORCID: ORCID
Nadia Drobyk
2
Daria Navrotska
1
ORCID: ORCID
Nataliia Nuzhyna
3
ORCID: ORCID
Oksana Poronnik
1
ORCID: ORCID
Iryna Konvalyuk
1
ORCID: ORCID
Ganna Myryuta
1
ORCID: ORCID
Roman Ivannikov
4
ORCID: ORCID
Ivan Parnikoza
1 5
ORCID: ORCID

  1. Department of Cell Population Genetics, Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, 150 Acad. Zabolotnogo Str., 03143, Kyiv, Ukraine
  2. Ternopil Volodymyr Hnatiuk National Pedagogical University, М. Kryvonosa str., 2, Ternopil, 46027, Ukraine
  3. Educational and Scientific Centre “Institute of Biology and Medicine” Kyiv Taras Shevchenko National University, 1 Symona Petlyury str., Kyiv, 01032, Ukraine
  4. M.M. Gryshko National Botanic Garden of National Academy of Sciences of Ukraine, 1 Timiryazevska str., 01014, Kyiv, Ukraine
  5. State Institution National Antarctic Scientific Center, Ministry of Education and Science of Ukraine, 16 Shevchenko Ave., 01601, Kyiv, Ukraine

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