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Number of results: 28
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Keywords fossilized bones
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Abstract

Fossilized bones help scientists not only identify the age of rocks, but also to determine the ancient environment and climate in which ancient humans and animals once lived. One just needs to know the right methods to use.

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Authors and Affiliations

Maciej T. Krajcarz
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Abstract

Introduction: Platelet-rich plasma (PRP) preparations can be used in bone tissue healing but there are numerous doubts among clinical orthopedists about effectiveness of this method.

Materials and methods: The studies were carried out in 12 rabbits of white termond breed. In operating room we operationally generated cylindrical, unicortical defects of the diameter of 4 mm in the middle of the shafts of both femurs. The defects in the left bones were left without filling and served as controls, and 0.7 ml of the ready-to-use PRP was administered to the defects in the right bones (experimental group). We evaluated the usefulness of the diagnostic methods applied: biomechanical tests, micro-CT tests, densitometry, typical radiology, macroscopic measurements, histopathological examinations.

Results: The macroscopic measurements showed a statistically significant increase in the dimension in the area of the right defect filled with PRP in relation to the control group. In experimented group, the assessment of the X-ray images showed the formation of a callus cuff around the defects. Densitometric examinations showed no statistically significant differences between defects in the experimental and control group. The analysis of the micro-CT examina- tions showed an increase in the total volume of the tissue examined (Vb) and the low density tissue fraction (Vb2) in the experimental group. The biomechanical examinations revealed signi- ficant decrease in the maximum breaking force (F max) necessary to break the bone in the experi- mental group in relation to the control group.

Conclusions: Platelet-rich plasma (PRP) stimulates bone formation in the area of bone defects and may accelerate bone regeneration.

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Authors and Affiliations

S. Skwarcz
I. Bryzek
A. Gregosiewicz
E. Warda
K. Gawęda
M. Tarczyńska
R. Węgłowski
J. Skwarcz
R. Nadulski
A. Starek
J. Sanford
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Abstract

It was assumed that the temperature at which caffeine is consumed may cause changes in the mechanical and morphological properties of bones and affect the bone metabolism of pregnant female. The aim of the study was to assess the effect of caffeine used at different temperatures on selected morphological and mechanical properties of the femur as well as biochemical indicators of bone formation in female rats in pregnancy. It was use test solution at the temperature 10, 25 and 45ºC from 8 to 21 day during pregnancy, once a day. It was found that the temperature of administrated caffeine may have an effect on changing the morphometric properties and on the bone metabolism of pregnant female rats. The application of caffeine solution, was administrated at 10ºC caused the most constrained growth of the femur and weakened resistance to load, and caused increased susceptibility to cracks. Analysis of bone metabolism indicators showed that caffeine administered in the form of a solution at 10ºC and 25ºC caused the most negativity effects for bone formation and bone turnover indicators. The administration of caffeine at 10ºC causes the largest negative changes in bone morphological and strength indicators and hasn’t a beneficial effect on the bone metabolism of pregnant female rats.
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Authors and Affiliations

Monika Cendrowska-Pinkosz
1
Wojciech Dworzański
1
Magdalena Krauze
2
Paulius Matuseviĉius
3
Katarzyna Ognik
2
Franciszek Burdan
1

  1. Department of Human Anatomy, Medical University in Lublin, Lublin, Poland
  2. Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Lublin, Poland
  3. Department of Animal Nutrition, Lithuanian University of Health, Kaunas, Lithuania
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Abstract

Chitosan (CS) is a polysaccharide readily used in tissue engineering due to its properties: similarity to the glycosaminoglycans present in the body, biocompatibility, non-toxicity, antibacterial character and owing to the fact that its degradation that may occur under the influence of human enzymes generates non-toxic products. Applications in tissue engineering include using CS to produce artificial scaffolds for bone regeneration that provide an attachment site for cells during regeneration processes. Chitosan can be used to prepare scaffolds exclusively from this polysaccharide, composites or polyelectrolyte complexes. A popular solution for improving the surface properties and, as a result enhancing cellbiomaterial interactions, is to coat the scaffold with layers of chitosan. The article focuses on a polysaccharide of natural origin – chitosan (CS) and its application in scaffolds in tissue engineering. The last part of the review focuses on bone tissue and interactions between cells and chitosan after implantation of a scaffold and how chitosan’s structure affects bone cell adhesion and life processes.
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Authors and Affiliations

Anna Kołakowska
1
ORCID: ORCID
Agnieszka Gadomska-Gajadhur
1
ORCID: ORCID
Paweł Ruśkowski
1
ORCID: ORCID

  1. Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
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Abstract

In Agricultural and Industrial Works FARMUTIL HS at Śmiłów were "cleaner production" program has been pul in practice, elimination of odors occurring during production of the meal and bone meal is of vital importance. Conccnlrations and emissions of lola! dust, organic substances in form of gas and vapors (as total organic carbon), hydrogen chloride, fluorine chloride, sulphur and nitrogen dioxides, carbon monoxide, heavy metals, polychlorinatcd dibcnzodioxins and dibcnzofurans were measured al emitters of the piani for thermal disposal of odors from the production of the meat and bone meal. The results of measurements and analyses of the composition of the flue gas emitted lo the atmosphere revealed that the concentration of harmful chemical compounds was low, lower than the permissible values defined in the standards.
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Authors and Affiliations

Zygmunt Kowalski
Anna Maślanka
Ewa Surowiec
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Abstract

This paper describes a unique case of craniosynostosis in a female skull in which sagittal sutures were completely fused by adolescence. Despite sagittal synostosis, the skull was of normal shape and size. Regarding craniometric features, the synostotic normocephalic skull was markedly different than that of scaphocephalic skulls which typically result from premature obliteration of the sagittal suture.
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Authors and Affiliations

Janusz Skrzat
1
Matthew J. Zdilla
2
Paweł Brzegowy
3
Jerzy Walocha
1

  1. Department of Anatomy, Jagiellonian University Medical College, Kraków, Poland
  2. Department of Pathology, Anatomy, and Laboratory Medicine (PALM), West Virginia University School of Medicine, Morgantown, West Virginia, USA
  3. Department of Radiology, Jagiellonian University Medical College, Kraków, Poland
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Abstract

The paper considers the application of statistical properties of backscattered ultrasonic signal for assessment of the trabecular bone status.

Computer simulations were conducted to investigate the properties of the ultrasound pulse-echo signal, as it is received on the transducer surface after scattering in trabecular bone. The micro-architecture of trabecular bone was modeled by a random distribution of long and thin cylindrical scatterers of randomly varying diameters and mechanical properties, oriented perpendicular to the ultrasound beam axis. The received echo signal was calculated as a superposition of echoes from all the scatterers present in the scattering volume.

The simulated signal envelope was used for statistical processing to compute various parameters like the mean amplitude, the amplitude MSR defined as the ratio of the mean to the standard deviation and the amplitude histogram.

Results indicated that while for the well-defined trabeculae properties within the simulated bone structure the signal envelope values are Rayleigh distributed the significant departures from Rayleigh statistics may be expected as the thickness of trabeculae become random. The influence of the variation of mechanical properties of the bone tissue building the trabeculae on the bone backscattered signal parameters was not observed.

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Authors and Affiliations

Jerzy Litniewski
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Abstract

Cytological evaluation of bone marrow smears stained by May-Grünwald Giemsa method was performed. The smears came from 20 fallow deer (Dama dama) 3 days old divided into 2 groups each consisting of 10 animals. The experimental group (E) received intramuscularly selenium and vitamin E at a dose of 3.0 ml (tocopherol acetate – 50 mg, sodium selenite – 0.5 mg, solvent - 1 ml) in the 3rd day of age. The control group (C) did not receive any supplementation or placebo. For hematological analyzes blood was collected three times: on 0, 15th and 25th day of the experiment. Serum concentration of selenium and vitamin E was determined using high perfor- mance liquid chromatography and glutathione peroxidase activity (GSH-Px) by kinetic method. On the 15th day after supplementation, a statistically significant increase in the percentage of erythroblastic cell line was observed in bone marrow smears. At that time, the increase in GSH-Px activity in the E group was also observed, reaching the value of 165.3 U/gHb, which was statisti- cally significant. The percentage of proerythroblasts (8.23% in group E and 5.02% in group C) differed significantly between groups at the 25th day after supplementation. This study revealed that supplementation of selenium and vitamin E resulted in an increase in the number of erythro- cytes to an average of 13.5 (˟ 10¹²/l) in the experimental group on 25th day with a significant increase in hemoglobin to 193 g/l in the experimental group.

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Authors and Affiliations

A. Snarska
D. Wysocka
L. Rytel
K. Żarczyńska
P. Sobiech
S. Gonkowski
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Abstract

This paper presents simulations of a three-dimensional model of the bone remodelling process. The model consists of a set of variable order partial differential equations, in which the varying order depends on the presence of tumour cells. The simulations are of a two-dimensional bone, to make visualisation simpler. They show that this model corresponds to the known evolution of bone remodelling, and is simpler than integer order models found in the literature.

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Authors and Affiliations

D. Valério
J. Neto
S. Vinga
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Abstract

An original method of skeletal system modelling is presented in detail. Using DICOM images obtained from CT and PET tests, shell models of nine bones were created (humerus, radius, ulna, scapula, clavicle, femur, tibia, fibula, pelvis). Two methods of bone behaviour are also proposed, the first method treating the bone as a solid structure and the second method treating the bone as a complex porous structure. The behaviour of model parts is numerically examined by using the finite element method.

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Authors and Affiliations

Henryk Olszewski
Wiktoria Wojnicz
Edmund Wittbrodt
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Abstract

The purpose of the study was to assess the effect of autologous activated platelet-rich plasma on healing of a bone substitute - tricalcium phosphate hydroxyapatite in experimental long bone defects using an animal model.

The experiment involved an animal model of femoral defect. 24 Termond white rabbits were used in the study. We evaluated the effect of autologous platelet-rich plasma on tricalcium phos- phate using classical radiology, micro-CT studies, strength tests and histological evaluation.

Radiological and histological assessment did not show a beneficial effect of PRP together with a bone substitute in comparison to filling the defects only with bone replacement material. The only benefit of adding platelet-rich plasma to a bone substitute was shown in microCT imaging.

Autologous, activated platelet-rich plasma combined with hydroxyapatite tricalcium phos- phate has a positive effect on the remodeling of the newly formed bone tissue, increasing its density.

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Authors and Affiliations

S. Skwarcz
I. Bryzek
A. Gregosiewicz
E. Warda
K. Gawęda
M. Tarczyńska
J. Skwarcz
R. Nadulski
A. Starek
J. Sanford
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Abstract

Wet shotcrete technology is being gradually used in roadway support in frigid mining areas. Thus, problems such as low strength, fragility, and high repair rate have also emerged. This study focuses on low strength, cracking, and other problems in the wet shotcrete support of a mine. It introduced the fishbone diagram to investigate the effects of temperature, cement content, and water-cement ratio (W/C) on the strength of the shotcrete layer. The microscopic morphology of wet shotcrete based on scanning electron microscopy (SEM) is observed. Results demonstrated that temperature was the main influencing factor of wet shotcrete in frigid mining areas. When the curing temperature was lower than 10°C, the early strength of wet shotcrete dropped significantly. Temperatures above 15°C were favorable for later gain in strength. W/C was of a complementary relationship with strength development at different ages. Temperature was the essential factor that influenced the microscopic morphology of wet shotcrete. Furthermore, internal initial porosity and aggregate interface bonding strength had a direct effect on macro-mechanical properties of wet shotcrete.

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Authors and Affiliations

Wei Sun
ORCID: ORCID
Zhang Shengyou
Xinglong Feng
Kepeng Hou
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Abstract

The anatomy of the human temporal bone is complex and, therefore, poses unique challenges for students. Furthermore, temporal bones are frequently damaged from handling in educational settings due to their inherent fragility. This report details the production of a durable physical replica of the adult human temporal bone, manufactured using 3D printing technology. The physical replica was printed from a highly accurate virtual 3D model generated from CT scans of an isolated temporal bone. Both the virtual and physical 3D models accurately reproduced the surface anatomy of the temporal bone. Therefore, virtual and physical 3D models of the temporal bone can be used for educational purposes in order to supplant the use of damaged or otherwise fragile human temporal bones.

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Authors and Affiliations

Janusz Skrzat
Matthew J. Zdilla
Paweł Brzegowy
Mateusz Hołda
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Abstract

Periodontitis is a highly prevalent, chronic immune-inflammatory disease of the periodontium that results in the periodontium and alveolar bone loss’s progressive destruction. In this study, the induction of periodontal disease via retentive ligature, lipopolysaccharide, and their combination at three different times were compared in a rat model. Seventy-two Sprague Dawley rats were distributed into four treatment groups: 1) control group with no treatment; 2) application of 4/0 nylon ligature around second maxillary molars; 3) combination of ligature and LPS injection (ligature-LPS); 4) intragingival injection of Porphyromonas gingivalis lipopolysaccharide ( Pg-LPS) to the palatal mucosa of the second maxillary molars. Six rats were sacrificed from each group after 7, 14, and 30 days of periodontal disease induction. Alveolar bone loss, attachment loss, number of inflammatory cells, and blood vessels were evaluated histologically. A micro-CT scan was used as a parameter to know the rate of alveolar bone loss. Parametric data were analyzed using two-way ANOVA followed by Bonferroni correction with a significance set at 5%. Non-parametric data were analyzed using Kruskal-Wallis, followed by multiple comparisons with Bonferroni correction. The histological results revealed significant destructive changes in the periodontal tissues and alveolar bone following the ligature and ligature-LPS induction techniques. These changes were evident as early as seven days, maintained until 14 days post-treatment, and declined with time. The ligature technique was effective in inducing acute periodontal disease. The LPS injection technique did not induce alveolar bone loss, and its combination to ligature added insignificant effects.
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Authors and Affiliations

H. Mustafa
1 2
C.H. Cheng
1
R. Radzi
1
L.S. Fong
1
N.M. Mustapha
1
H.O. Dyary
2

  1. Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  2. College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq
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Abstract

This study is an illustrative case of the ipsilateral ossification of the dural folds stretched between the clinoid processes found in the juvenile sphenoid bone. Thereby, occurred inconstant bony formations are termed as the interclinoid bridge and the carotico-clinoid foramen.
Both structures are of clinical meaning if surgical operations are performed in the sellar region.
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Authors and Affiliations

Janusz Skrzat
1
Grzegorz Goncerz
1
Agnieszka Szczepanek
1
Magdalena Kozerska
1

  1. Department of Anatomy, Jagiellonian University Medical College, Kraków, Poland
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Abstract

A trabecular bone consists of trabeculae whose mechanical properties differ significantly from the surrounding marrow, therefore an ultrasonic wave propagating within the bone structure is strongly scattered. The aim of this paper was to evaluate the contribution of the first, second and higher order scattering (multiple scattering) into the total scattering of ultrasound in a trabecular bone. The scattering due to the interconnections between thick trabeculae, usually neglected in trabecular bone models, has been also studied. The basic element in our model of the trabecular bone was an elastic cylinder with a various finite-length and diameter as well as orientation. The applied model was taking into account variation of both, elements size and their spatial configuration. The field scattered on the bone model was evaluated by solving numerically the integral form of the generalized Sturm-Liouville equation describing a scalar wave in inhomogeneous and lossy media. For the scattered fields calculated numerically the effective cross-sections were determined. The influence of absorption on the scattering coefficients was demonstrated. The results allowed to conclude that within the frequency range from 0.5 to 1.5 MHz contribution of the second order scattering to the effective backscattering cross-section is at least 500 times lower than that due to the first order scattering. It was noticed that for a frequency higher than 1.5 MHz fast growth of the backscattering (reflection) coefficients, calculated for the second order scattering, occurs.

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Authors and Affiliations

Andrzej Nowicki
Janusz Wójcik
Jerzy Litniewski
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Abstract

We measured the bone-specific alkaline phosphatase (ALP) isoenzyme activity in 67 plasma samples from 14 newborn Holstein calves using both a conventional method (featuring heat inactivation) and a commercial agarose gel electrophoresis (AGE) kit; the relevant isoenzymes were termed bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3). We explored whether the AGE kit afforded reliable data when used to analyze samples from Holstein calves. The blood was collected from the jugular vein of each calf immediately prior to the first colostrum feeding (pre-feeding), 20 and 40 h after pre-feeding, and on days 4 and 7; whereas three samples (from three calves) were not obtained. The total plasma ALP activity varied widely, exceeding the ranges of reference values. On electrophoresis, 52 of 67 plasma samples (77.6 %) clearly contained both ALP isoenzyme 2 and ALP3, as did control human serum. The total ALP activity of the 52 samples ranged from 166–1989 U/L (median: 1013 U/L), whereas the values for the other 15 samples (22.4%) exhibiting abnormal isoenzyme fractionation ranged from 1014–5118 U/L (median: 1780 U/L). In the 52 plasma samples exhibiting clearly separated isoenzymes, ALP3 and BAP activities were strongly positively correlated as revealed by Deming regression (y = 0.93x + 22.6, p<0.0001) and Bland-Altman analysis (ALP3/BAP activities limit of agreement: −5.1%). Thus, the AGE kit yields useful information on newborn calves, and can replace the conventional method when the total plasma ALP activity is less than approximately 1000 U/L.

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Authors and Affiliations

R. Onomi
K. Hatate
N. Yamagishi
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Abstract

The study was undertaken to determine the effect of continuation or changes of the diet on the morphometry and histomorphometry of bone in male and female Wistar rats with experimen- tally induced obesity by high energetic diet. Sixty-four 90-day-old Wistar rats obtained from obese parents (16 male, 16 female) and control parents (16 male, 16 female) were used in this study. After 21 days of the baby period, rats were divided into four groups: obese rats fed with high energy feed (F/F), control rats fed with a standard diet (C/C), obese rats with changed diet from high energy diet to control diet (F/C) and control rats with changed diet from control diet to high energy diet (C/F). After 90 days of experimental feeding, the rats were sacrificed. Thereafter, body weight and the isolated humerus were measured and next, the histological stainings and counts were done. Our results revealed that change in the parent’s diet from F to C in the female leads to increased bone growth length and reduction of body weight in female and male. Reverse diet changes (from C to F) lead to decreased bone length only in the female. Moreover, the con- tinuation by offspring of both sexes with a high-energy diet contributes to a reduction in osteo- cytes, reduction in bone marrow cavity and cortical expansion, but a change in nutrition from parents’ standard diet to high-energy diet leads to increase in osteocytes dimensions. The contin- uation of feeding with F diet promotes the accumulation of adipocytes in the bone marrow in female and male, and correction of nutrition from F to standard diet leads to a reduction in their number in the bone marrow compared to groups continuing feeding with high-energy diet.

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Authors and Affiliations

D. Wolski
A. Wawrzyniak
M. Bieńko
J. Michalik
R.P. Radzki
M.B. Arciszewski
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Abstract

One of the most common reasons for horse lameness is subchondral bone cysts (SBCs), which are especially evident in young horse athletes. It is believed that SBC development is strongly associated with an individual’s bone growth and/or bone microstructure impairment. Current methods of SBC treatment include pharmacological treatment or surgical procedures which may allow the bone within the cyst to rebuild and be restored to properly developed bone tissue. Thus, we propose filling the SBCs with a 3D complex of alginate hydrogel and autologous adipose derived mesenchymal stem cells (ASCs). We have observed at the in vitro level, that this hydrogel complex induces osteogenic and chondrogenic differentiation potential through the upregulation of bone morphogenetic protein, osteopontin, collagen type I and aggrecan mRNA levels. Moreover, we detected the creation of a 3D extracellular matrix (EM). To investigate the complex in vivo, we chose 8 horses of varying age suffering from SBC, which resulted in lameness, to undergo experimental surgery. We documented the horses’ clinical appearance, lameness and radiographic appearance, to determine that there was clinical improvement in 87.75% of the patients (n=7, out of 8 horses) 6 months postoperatively and 100% (n=8, out of 8 horses) a year after surgery. These results are promising for the potential of this procedure to become the standard in SBC treatment.
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Authors and Affiliations

P. Golonka
M. Szklarz
M. Kusz
M. Marędziak
J.M. Irwin Houston
K. Marycz
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Abstract

Authors paid attention to anatomy and clinical implications which are associated with the variations of the sphenoid sinus. We discuss also anatomical structure of the sphenoid bone implementing clinical application of this bone to diff erent invasive and miniinvasive procedures (i.e. FESS).

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Authors and Affiliations

Joanna Jaworek-Troć
Michał Zarzecki
Anna Bonczar
Lourdes N. Kaythampillai
Bartosz Rutowicz
Małgorzata Mazur
Jacenty Urbaniak
Wojciech Przybycień
Katarzyna Piątek-Koziej
Marcin Kuniewicz
Marcin Lipski
Wojciech Kowalski
Janusz Skrzat
Marios Loukas
Jerzy Walocha
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Abstract

The objective of the study was to create a printable 3D model of the sellar region of the sphenoid bone for demonstrating anatomical variant of the osseous bridging between anterior and posterior clinoid processes. Three-dimensional reconstruction of the middle cranial fossa along with 3D printed model, allow for accurate depicting position of the interclinoid bridge with reference to other basicranial structures.

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Authors and Affiliations

Janusz Skrzat
Anna Bonczar
Tomasz Kasprzycki
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Abstract

The paper presents a method of analysis of bone remodelling in the vicinity of implants. The authors aimed at building a model and numerical procedures which may be used as a tool in the prosthesis design process. The model proposed by the authors is based on the theory of adaptive elasticity and the lazy zone concept. It takes into consideration not only changes of the internal structure of the tissue (described by apparent density) but also surface remodelling and changes caused by the effects revealing some features of “creep”. Finite element analysis of a lumbar spinal segment with an artificial intervertebral disc was performed by means of the Ansys system with custom APDL code. The algorithms were in two variants: the so-called site-independent and site-specific. Resultant density distribution and modified shape of the vertebra are compared for both of them. It is shown that this two approaches predict the bone remodelling in different ways. A comparison with available clinical outcomes is also presented and similarities to the numerical results are pointed out.

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Authors and Affiliations

Paweł Wymysłowski
Tomasz Zagrajek
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Abstract

Analysis of weathering parameters of bones from cave deposits is presented as a useful tool of palaeoenvironmental reconstruction. As an example, we studied profiles of sediments in two Palaeolithic sites: Nietoperzowa Cave and Deszczowa Cave. Our studies included histological and EDS analyses of bone remnants found in these profiles. This method allowed us to reconstruct the changes of palaeotemperature and palaeohumidity, and finally the climatostratigraphy of sediments. The results presented here put a new light onto the stratigraphy of Deszczowa Cave's filling. In particular, besides the Vistulian sediments (MIS 2-5d), we confirmed the presence oflayers formed during the Penultimate Glaciation (MIS 6) and Eemian Interglacial (MIS 5e).

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Authors and Affiliations

Maciej T. Krajcarz
Teresa Madeyska

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