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Molecular identification of Babesia spp isolated from Polish cattle with asymptomatic protozoa infections

M. Staniec Ł. Adaszek K. Buczek S. Winiarczyk
Polish Journal of Veterinary Sciences | 2018 | vol. 21 | No 2 | 287–291 | DOI: 10.24425/119050
Keywords babesiosis cattle sequencing
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Abstract

The aim of the paper was to study the epizootic situation of babesiosis in the cattle population in eastern Poland and possibly to determine what species of protozoa infects Polish cattle. Blood samples for molecular analysis (real time PCR) were collected from 192 dairy cows from various farms located in eastern Poland. The infection was detected in 10.4% of the samples. All animals were infected with Babesia occultans which sequence of the 18S RNA gene fragment showed a 93.1%, homology with the sequence of B. occultans EU 376017. This is the first report about the detection of B. occultans DNA in asymptomatic cattle in eastern Poland.
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Authors and Affiliations

M. Staniec
Ł. Adaszek
K. Buczek
S. Winiarczyk

Prevalence and major causes of ruminal lesions in intensive cattle fattening units

J. Vieira R. Matos J. Cannas da Silva W. Baumgartner S. Van Harten A. Dâmaso
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 4 | 635-637 | DOI: 10.24425/pjvs.2022.143547
Keywords ruminal lesions ruminal acidosis cattle feedlot management
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Abstract

Ruminal acidosis is frequent in cattle fattening systems associated with grain-rich fibre-poor diets. This study aimed to assess the prevalence of ruminal lesions compatible with ruminal acidosis intensive fattening farms and its risk factors on farm. Rumens of 218 out of 1960 bullocks from six farms were classified in slaughterhouses, and the management practices and the type of feed were compared. The prevalence of lesions compatible with ruminal acidosis was high (65%), where 47% and 18% of the animals had ruminal lesions compatible with subacute and acute disease, respectively. Prevalence of lesions differed significantly between the farms, with differences in the management and feeding practices. Ruminal lesions compatible with ruminal acidosis are prevalent in cattle fattening units in Portugal and husbandry and feeding practices are likely to have an impact.
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Authors and Affiliations

J. Vieira
1
R. Matos
1 2
J. Cannas da Silva
1 2
W. Baumgartner
3
S. Van Harten
1 2
A. Dâmaso
1 2
  1. Veterinary Medicine School, University Lusófona of Humanities and Technologies, Campo Grande 376, 1749-024, Lisbon, Portugal
  2. CBIOS - Research Centre for Biosciences & Health Technologies, Campo Grande 376, 1749-024, Lisbon, Portugal
  3. University Clinic for Ruminants, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria

The influence of short-term selenitetriglycerides supplementation on blood selenium, and hepatic, renal, metabolic and hematological parameters in dairy cows

K. Żarczyńska P. Sobiech J. Mee J. Illek
Polish Journal of Veterinary Sciences | 2020 | vol. 23 | No 4 | 637–646 | DOI: 10.24425/pjvs.2020.135812
Keywords selenium selenitetriglycerides cattle biochemical parameters haematology
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Abstract

Selenium deficiency is a common nutritional disorder in dairy cattle globally. However, sele- nium supplementation can lead to selenium toxicity. This study evaluated a novel, low-toxicity selenium supplement, selenitetriglycerides, to determine its efficacy and safety in dairy cows. The study was conducted on 12 Holstein Friesian cows divided in two equal groups (control group without supplementation of selenium and experimental group with supplementation of selenitetriglycerides). Experimental cows (n=6) were orally administered 300 mg/cow/day of selenitetriglycerides for 14 days (days 1-14) and then monitored for a further 14 days (days 15-28). Blood from both groups of cows was sampled for determination of selenium concentra- tions, activity of aspartate aminotransferase, creatine kinase, lactate dehydrogenase, gamma-glutamyl transferase, concentrations of triglycerides, cholesterol, non-esterified fatty acids, glucose, total protein, urea, creatinine and hematological parameters. Serum selenium concentra- tions in the experimental group increased significantly on day 2 (from 64.92±6.89 μg/L to 127.95±13.75 μg/L), peaked on day 7 (266.22±14.21 μg/L) and remained significantly above the initial baseline values (day 1) for 28 days. Serum selenium concentrations in the control group did not change significantly during the 28 day period (65.22 μg/L on 1st day and 64,35 μg/L on 28th day) and were significantly lower than those in the experimental group from day 2 to day 28. The results of clinical examinations, analyses of hematological parameters, and liver and kidney function tests showed that selenitetriglycerides had no adverse effect on the health or on the metabolic or haematological statuses of the cows. These findings indicate that sele- nitetriglycerides are safe and effective selenium supplements for cattle.

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Authors and Affiliations

K. Żarczyńska
P. Sobiech
J. Mee
J. Illek

The effect of DDE and dieldrin and their parental substances (DDT and aldrin) on PGE2 and PGF2α release from bovine endometrial explants collected during 120 to 180 days of the gestational period

J. Mlynarczuk M.H. Wrobel K. Dobrzyn
Polish Journal of Veterinary Sciences | 2021 | vol. 24 | No 1 | 5-12 | DOI: 10.24425/pjvs.2020.135813
Keywords pesticides pregnancy endometrium prostaglandin secretion cattle
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Abstract

Dieldrin and DDE are environmental metabolites of the organochlorine pesticides aldrin and DDT, respectively. During pregnancy, these chemicals can quickly infiltrate through the placental barrier, accumulate in amniotic fluid and fetus, and act as endocrine disruptors (EDs). The aim of this study was to investigate the effect of DDE and dieldrin and their parental substances at concentrations of 1 and 10 ng/ml on secretion of PGE2 and PGF2α from bovine endometrial explants (120-150 and 151-180 days of pregnancy) after 24 hr of incubation with EDs. The mRNA expression of COX2, PGES and PGFS and the concentrations of PGE2 and PGF2α were measured. EDs did not affect (p>0.05) COX2 gene expression, but DDT and DDE decreased (p<0.05) PGES expression and PGE2 secretion in the explants from 120-150 days of pregnancy. Depending on the dose, DDT and DDE increased (p<0.05) PGFS expression and PGF2α secretion from the explants from 120-150 days and decreased PGF2α secretion (p<0.05) from the explants from 151-180 days of pregnancy. Aldrin and dieldrin decreased (p<0.05) PGFS expression and PGF2α secretion from all explants. In summary, EDs disrupt the secretion of PGE2 and PGF2α by influencing the gene expression of PGES and PGFS.
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Bibliography

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Authors and Affiliations

J. Mlynarczuk
1
M.H. Wrobel
1
K. Dobrzyn
1
  1. Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Department of Physiology and Toxicology of Reproduction, Tuwima 10, 10-747 Olsztyn, Poland

Sire pedigree error estimation and sire verification of the Taiwan dairy cattle population by using SNP markers

C.H. Chao Y.H. Yeh Y.M. Chen K.H. Lee S.H. Wang T.Y. Lin
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 1 | 61-65 | DOI: 10.24425/pjvs.2022.140841
Keywords Holstein cattle genetic testing sire pedigree
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Abstract

Information regarding the correct pedigree of and relationship between animals is useful for managing dairy breeding, reducing inbreeding, estimating breeding value, and establishing correct breeding programs. Additionally, the successful implementation of progeny testing is crucial for improving the genetics of dairy cattle, which depends on the availability of correct pedigree information. Incorrect pedigree information leads to bias in bull evaluation. In this study, Neogen GeneSeek Genomic Profiler (GGP) 50K SNP chips were used to identify and verify the sire of Taiwanese Holstein dairy cattle and analyze the reasons that lead to incorrect sire records. Samples were collected from 2,059 cows of 36 dairy farms, and the pedigree information was provided by breeders. The results of sire verification can be divided into three categories: submitted unconfirmed sire, submitted confirmed sire, and incorrectly submitted verified sire. Data on the sires of 1,323 (64.25%) and 572 (27.78%) dairy cows were verified and discovered, respectively. Sires of 1,895 (92.03%) dairy cattle were identified, which showed that the paternal pedigree of dairy cattle could be discovered and verified through genetic testing. An error-like analysis revealed that the data of 37 sires were incorrectly recorded because the bull’s NAAB code number was incorrectly entered into the insemination records: for 19 sires, the wrong bull was recorded because the frozen semen of a bull placed in the wrong storage tank was used, 6 had no sire records, and for 12 sires, the NAAB code of the correct bull was recorded but with a wrong stud code, marketing code, or unique number for the stud or breed. To reduce recorded sire error rates by at least 27.78%, automated identification of the mated bull must be adopted to reduce human error and improve dairy breeding management on dairy farms.
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Bibliography


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Authors and Affiliations

C.H. Chao
1
Y.H. Yeh
1
Y.M. Chen
1
K.H. Lee
1
S.H. Wang
1
T.Y. Lin
1
  1. Hsinchu Branch, Livestock Research Institute, Council of Agriculture, Executive Yuan, 207-5, Bi-tou-mian, Wu-hoo village, Si-hoo Township, Miaoli County, Taiwan

ADM1-based modeling of anaerobic codigestion of maize silage and cattle manure – a feedstock characterisation for model implementation (part I) / Modelowanie kofermentacji kiszonki kukurydzy i obornika bydlęcego za pomocą ADM1 – charakterystyka wsadu surowcowego (część I)

Ewa Klimiuk Zygmunt Mariusz Gusiatin Tomasz Pokój Sabina Rynkowska
Archives of Environmental Protection | 2015 | vol. 41 | No 3 | DOI: 10.1515/aep-2015-0026
Keywords cattle manure maize silage anaerobic digestion ADM1 modeling
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Abstract

This paper presents the results of fractionation of particulate and soluble organic matter in a mixture of maize silage and cattle manure (49:51% volatile solids) that was used as a feedstock for anaerobic digestion. The extended Weender’s analysis was adapted to measure raw protein, raw lipids, fraction of carbohydrates (including starch, cellulose, hemicelluloses) and lignin. The content of individual fractions in composite, Xc (as kg COD kg-1 COD) was: 0.111 proteins, 0.048 lipids, 0.500 carbohydrates and 0.341 inerts. The biodegradability of Xc was 68%. Based on material balance, the carbon concentration in Xc was 0.0326 kmol C kg-1 COD, whereas nitrogen concentration 0.0018 kmol N kg-1 COD. The estimated pH of the feedstock based on acid-base equilibrium corresponded to the actual value (pH 7.14).

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Authors and Affiliations

Ewa Klimiuk
Zygmunt Mariusz Gusiatin
Tomasz Pokój
Sabina Rynkowska

ADM1-based modeling of anaerobic codigestion of maize silage and cattle manure – calibration of parameters and model verification (part II) / Modelowanie kofermentacji kiszonki kukurydzy i obornika bydlęcego za pomocą ADM1 – kalibracja i weryfikacja modelu (część II)

Katarzyna Bułkowska Ireneusz Białobrzewski Zygmunt Mariusz Gusiatin Ewa Klimiuk Tomasz Pokój
Archives of Environmental Protection | 2015 | vol. 41 | No 3 | DOI: 10.1515/aep-2015-0027
Keywords cattle manure maize silage anaerobic digestion ADM1 modeling
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Abstract

The aim of this study was to implement ADM1xp model to simulate behavior of anaerobic co-digestion of maize silage and cattle manure. The accuracy of ADM1xp has been assessed against experimental data of anaerobic digestion, performed at OLR = 2.1 gVS dm-3·d-1 and HRT = 45d. Due to the high number of parameters in ADM1xp, it was necessary to develop a customized procedure limiting the range of parameters to be estimated. The best fitting of experimental to simulated data was obtained after verification of 9 among 105 stoichiometric and kinetic parameters. The values of objective function (Jc) ranged between 0.003 (for valerate) and 211 (for biogas production).

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Authors and Affiliations

Katarzyna Bułkowska
Ireneusz Białobrzewski
Zygmunt Mariusz Gusiatin
Ewa Klimiuk
Tomasz Pokój

The elimination of lead(II) ions in a solution by bio-adsorption: Kinetics, equilibrium, and thermodynamics

Candelaria Tejada-Tovar Humberto Bonilla-Mancilla Rodrigo Ortega Toro Ángel Villabona-Ortíz Manuel Díaz-Illanes
Journal of Water and Land Development | 2022 | No 53 | 118-127 | DOI: 10.24425/jwld.2022.140787
Keywords bio-adsorption cattle manure heavy metals isotherm of Langmuir thermodynamic parameters
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Abstract

In the present study, the removal capacity of Pb(II) ions was investigated using the biomass of dried cattle manure in an aqueous solution. The biomaterials were characterized using Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDS) techniques. The results reveal that the adsorption mechanism may be associated with the interaction between Pb(II) ions and functional groups through aggregation, coordination, ion exchange, microprecipitation, oxidation, and hydrophobicity. The bio-adsorption of the metal was analysed in discontinuous tests; the effect of temperature, pH, agitation, and adsorbent dose was evaluated. The maximum adsorption capacity was determined at pH 7.5, 18°C and 200 rpm. The bio-adsorption of Pb(II) was best fitted to the pseudo-second order model. The experimental data of the isotherm were adjusted to the models of Langmuir, Freundlich and Dubinin–Radushkevich; while Langmuir’s model related better to the experimental data forming a single layer at saturation. The rate of adsorption was rapid, reaching equilibrium after 25 min and removal of 96.8%. Thermodynamic parameters determined that the process was viable, spontaneous, and exothermic. The present study contributes mainly to demonstrating that a biomaterial prepared from bovine manure is a promising adsorbent for heavy metals such as Pb(II). It also reduces the environmental impact of this waste through the generation of greenhouse gases in countries that maintain intensive livestock. Another important aspect is the reduction of the micro- and macronutrients accumulation in soil and contamination of surface waters and aquifers by runoff and seepage during rainy periods.
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Authors and Affiliations

Candelaria Tejada-Tovar
1
ORCID: ORCID
Humberto Bonilla-Mancilla
2
ORCID: ORCID
Rodrigo Ortega Toro
3
ORCID: ORCID
Ángel Villabona-Ortíz
1
ORCID: ORCID
Manuel Díaz-Illanes
2
ORCID: ORCID
  1. Universidad de Cartagena, Department of Chemical Engineering, Cartagena de Indias, Colombia
  2. Universidad Nacional del Centro del Perú, Faculty of Forestry and Environmental Sciences, Huancayo, Peru
  3. Universidad de Cartagena, Department of Food Engineering, Av. del Consulado St. 30 No. 48-152, 130001, Cartagena de Indias, Colombia

Molecular epidemiology and phylogenetic analysis of Tams I gene of Theileria annulata in Khyber Pakhtunkhwa, Pakistan

S.S.A. Shah U. Saddique M.A. Khan S. Khan
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 4 | 625-629 | DOI: 10.24425/pjvs.2022.143543
Keywords crossbred cattle Pakistan PCR risk factors sequencing theileriosis
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Abstract

Theileriosis is a hemoparasitic disease that affects a wide range of different animal species and is caused by various species of Theileria. This study aimed to determine the molecular epidemiology of Theileria annulata through microscopy and PCR, in crossbred cattle in some districts of Khyber Pakhtunkhwa, Pakistan. For this study, a total of 384 blood samples were collected from cattle in the Peshawar (n=120), Charsadda (n=94), Nowshera (n=84), and Swabi (n=86) districts. Microscopy and PCR were used to determine the overall prevalence of theileriosis, which was found to be 15.8 and 22.6%, respectively. Theileria annulata was detected in blood samples through PCR in the study area, and the target gene i.e., Tams 1, of positive samples was sequenced. The sequences in the current study revealed high sequence homology (ranging from 96 to 100%) with Tams 1 sequences of neighboring countries present in the NCBI database. Season, breed, age, and sex were found to be important risk factors among the several risk factors examined, whereas, among different clinical manifestations, lymphadenopathy showed a strong association with theileriosis. According to Cohen’s kappa and ROC analysis, microscopy was proven to be a fair diagnostic test for detecting theileriosis in cattle, and may be used in combination with molecular techniques for screening a large number of animals.
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Authors and Affiliations

S.S.A. Shah
1 2
U. Saddique
1
M.A. Khan
1
S. Khan
1
  1. College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, 25130, Khyber Pakhtunkhwa, Pakistan
  2. Veterinary Research Institute, Peshawar-Pakistan

Selected ruminal variables and purine urinary excretion rate of steers subjected to feeding, fasting, and re-feeding conditions

E.L. Ortolani C.A. Maruta R.A. Barrêto Júnior C.S. Mori A.C. Antonelli M.C.A. Sucupira A.H.H. Minervino
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 1 | 175-182 | DOI: 10.24425/pjvs.2022.140854
Keywords rumen feed deprivation pH allantoin excretion cattle
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Abstract

The effects of feeding, fasting, and re-feeding on the ruminal profile of growing cattle were studied. Ruminal fluid and urine samples were obtained from 12 crossbred steers weighing approximately 300 kg during the following periods: 11 h of normal feeding (postprandial period), 48 consecutive hours of fasting, and followed by 48 h of re-feeding. Fasting promotes changes in the ruminal profile, such as an increase in ruminal pH, reduction in the number of rumen protozoa and bacteria, and decrease in the urinary excretion of allantoin; however, it does not change the urinary uric acid excretion rate. The overall mean ruminal pH was higher during fasting (7.53±0.27) in comparison to those at normal feeding (6.72±0.25) and re-feeding (6.62±0.31) (p<0.05). During re-feeding, the ruminal profile returned to normal, except for the protozoa count, which despite a slight increase only after 48 h of re-feeding, did not recover to baseline values.
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Bibliography

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Authors and Affiliations

E.L. Ortolani
1
C.A. Maruta
1
R.A. Barrêto Júnior
2
C.S. Mori
1
A.C. Antonelli
3
M.C.A. Sucupira
1
A.H.H. Minervino
4
  1. Department of Clinical Science, School of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo-SP, 05508-270, Brazil
  2. Department of Animal Science, Federal University of Semiarid, 59625-900, Mossoró-RN, Brazil
  3. School of Veterinary Medicine, Federal University of Sao Francisco Valley, 56304-205, Petrolina-PE, Brazil
  4. Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, 68040-255 Santarém-PA, Brazil

CD4- and CD8-expressing cells found in the bovine and porcine anterior chamber of the eye

H. Ziółkowski T. Maślanka P. Socha N. Ziółkowska M. Dąbrowski J. Małaczewska
Polish Journal of Veterinary Sciences | 2018 | vol. 21 | No 2 | DOI: 10.24425/122596
Keywords anterior chamber CD4+ cells CD8+ cells DP cells cattle pig
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Abstract

The aim of the present study was to investigate whether the anterior chamber constitutes part of the normal migratory pathway of CD4+ and CD8+ lymphocytes in cattle and swine. The cells obtained from aqueous humor of cows and pigs were stained for CD4 and CD8 receptors, and subsequently analyzed with flow cytometry. The mean percentage of CD4+CD8-, CD4-CD8+ and CD4+CD8+ cells within the total lymphocyte population of the bovine anterior chamber was, respectively, 17.88, 12.64 and 27.26%. In turn, the mean values of these parameters in pigs were 1.77, 38.48 and 17.45, respectively. Among bovine and porcine CD4+CD8+ cells prevalent were those displaying CD4lowCD8low and CD4lowCD8high phenotypes, respectively. The results suggest that the anterior chamber in cattle and swine is an element in the normal migratory pathway of CD4+, CD8+ and CD4+CD8+ cells. Furthermore, the contribution of these subsets in the anterior chamber lymphocyte population can differ considerably between animal species.
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Authors and Affiliations

H. Ziółkowski
T. Maślanka
P. Socha
N. Ziółkowska
M. Dąbrowski
J. Małaczewska

Relationships between eye fluids and blood values after exercise in lidia cattle: mineral parameters

J.R. González-Montaña F. Escalera-Valente J.M. Lomillos A.J. Alonso V. Gaudioso M.E. Alonso
Polish Journal of Veterinary Sciences | 2019 | vol.22 | No 3 | 445–455 | DOI: 10.24425/pjvs.2019.129304
Keywords aqueous humour vitreous humour minerals parameters intraocular fluids blood metabolism Lidia cattle
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Abstract

Eye fluids (aqueous humour and vitreous humour) may be helpful in estimating ante-mortem blood levels, since some parameters measured in these fluids have proved to be stable or to change in a predictable way after death. This would help in diagnosing the cause of death in some diseases or to evaluate ante-mortem blood levels in certain animals not easy to handle or with difficult access. In order to establish reference values of some parameters in blood and eye fluids (aqueous humour and vitreous humour), as well as the possible correlation among these three different fluids, various minerals and electrolytes (Ca, P, Mg, K, Na, Fe, Cr, Co, Ni, Cu, Zn, Se and Mo) were measured in 15 four to five year-old Lidia bulls, all dying after a period of significant stress and major exertion. Plasmatic values of Mg and P were much greater than reported in the literature. In general, mineral plasmatic values were greater than those found in ocular fluids (aqueous and vitreous), while Na, K and Cr were similar in the three fluids. We have verified the existence of correlations in P, Co and Mo among the three fluids measured, and between Se of plasma and vitreous humour. But the most marked correlations were observed in Mo (plasma -aqueous humour, r = 0.893, plasma-vitreous humour, r = 0.945, HA -HV, r = 0.849), in P (plasma-vitreous humour, r = 0.726) and in Co (plasma-vitreous humour, r = 879).

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Authors and Affiliations

J.R. González-Montaña
F. Escalera-Valente
J.M. Lomillos
A.J. Alonso
V. Gaudioso
M.E. Alonso

Ultrasonographic image of fatty infiltration of the liver correlates with selected biochemical parameters and back fat thickness of periparturient Holstein-Friesian cows

D. Grzybowska P. Sobiech D. Tobolski
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 723-732 | DOI: 10.24425/pjvs.2023.148292
Keywords blood biochemistry dairy cattle fatty liver disease ketosis liver ultrasound subcutaneous fat transition period
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Abstract

During the transition period, the cow’s body activates adaptive mechanisms aimed at adjusting to the changing demand for energy and nutrients, which are necessary for the growing fetus and the subsequent start of milk production. This time is also associated with an increased risk of metabolic diseases and reproductive disorders.
Our study aimed to identify prepartum and postpartum biochemical markers and weight loss patterns that could differentiate cows that would exhibit ultrasonographic signs of liver fatty infiltration during the latter half of the transition period.
The study was performed in a single herd of Holstein-Friesian cows and the animals were divided into two groups: CON (n=13) – cows without ultrasonographic signs of fatty liver, and FL (n=16) – cows with ultrasonographic signs of fatty liver. Backfat thickness and specific biochemical parameters were measured weekly from one week before parturition to 9 weeks postpartum.
Our study highlights the importance of using a combination of monitoring methods to assess the metabolic status of transition dairy cattle. The results showed that ultrasound measurements of backfat thickness, blood NEFA levels, glucose concentration, and AST activity were all different (p<0.05) between the control and FL groups, indicating the usefulness of these parameters in monitoring the health status of transition cows. Additionally, the results suggest that high prepartum glucose levels (4.99 mmol/l) could serve as a potential marker for future FL, while the elevated NEFA levels (0.51 mmol/l) and decreased AST activity (80.56 u/l) in FL animals indicate their potential as indicators of lipid mobilization and liver structural damage, respectively.
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Authors and Affiliations

D. Grzybowska
1
P. Sobiech
1
D. Tobolski
1
  1. Department and Clinic of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

Characterization of class 1, 2, 3 integrons, ESBL genes and antibiotic susceptibility of Salmonella serotypes from broiler and cattles in Turkey

O. Sahan Yapicier D. Ozturk
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 2 | 345-355 | DOI: 10.24425/pjvs.2022.141820
Keywords antibiotic susceptibility broiler cattle integrons β-lactamase MARI Salmonella tet genes
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Abstract

Antimicrobial resistance in Salmonella has been associated with the presence of integrons and many other resistance mechanisms contributing to the spread of antimicrobial-resistant genes within and between livestock and human populations. In this study, the presence of Salmonella serovars from broiler and cattle samples and their antimicrobial resistance, integrons, tet resistance, ESBL and resistance genes carriage were investigated. Total of 209 litter (broiler farms) and fecal samples (cattle farms) were examined by bacteriological procedures, susceptibilities against 18 antimicrobials and genes carriages were detected by singleplex and multiplex PCR. A total of 46/209 (22 %) Salmonella strains were isolated. Six different Salmonella serotypes from 46 Salmonella isolates were identified and the most common serotype was S. Infantis 38 (82.6%) from broiler litter; followed by S. Kitenge 3 (6.5 %) from fecal sample. The highest occurrence of resistance observed for penicilline (46/46, %100), lincomycin (43/46, 93.5%) and 42 isolates (43/46, 93.5%) exhibited MDR. The overall occurrence of class 1, 2 and 3 integrons carrying Salmonella in tested samples were 63.04% (29/46), 43.5% (20/46) and 84.8% (39/46) respectively. Out of the 27 isolates produced an ESBL, mostly CTX and TEM. On 46 Salmonella isolates, in 16 (34.8%) Tcr’ genes were determined. Genotypic and phenotipic detection of ESBL genes found within integrons from Salmonella isolates from different sources (broiler and cattle) can provide powerful information about health and economic risk associated with transferable multidrug resistance.
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Authors and Affiliations

O. Sahan Yapicier
1
D. Ozturk
2
  1. Republic of Turkey Ministry of Agriculture and Forestry Veterinary Control Central Research Institute, Bacteriological Diagnostic Laboratory, 06020, Ankara, Turkey
  2. Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, 15030, Burdur, Turkey

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