According to The European Commission’s regulation numbers 842/2006 and 517/2014, refrigerants whose Global Warming Potential ratio is more than 150, have been prohibited in mobile air conditioning (MAC) since January 2017. Therefore, the commonly used R-134 gas has been banned. The search for a new refrigerant, which grants all the required criteria, has begun. In accordance with new European standards, the gas should have environmentally friendly properties and should not be noxious to human life while operating. In this paper, two alternative substances, which can substitute the banned R134a, have been compared. This is synthetic R1234yf, which belongs to the HFO group, and carbon dioxide, which exists in the natural environment. The chemical build, physical and thermodynamic properties have been described. Scientific articles, which present and compare the technical results of testing both refrigerants, have been discussed. Comparison results, tools used and research methodology have been described in these articles. Alternative gases have been analyzed for their environmental impact and have been checked on the toxic, flammable, impact on ozone depletion and global warming. The threats to human life due to the use of the new refrigerants have been reviewed. The thesis also comprises an economical comparison between the two gases. A short review and conclusions have been presented at the end of the article.