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Abstract

The hydrolysis of lignocellulosic biomass results in the production of so-called fermentation inhibitors, which reduce the efficiency of biohydrogen production. To increase the efficiency of hydrogen production, inhibitors should be removed from aqueous hydrolysate solutions before the fermentation process. This paper presents a new approach to the detoxification of hydrolysates with the simultaneous formation of in-situ deep eutectic solvents (DES). In the first stage of the study, inhibitors were identified in the real hydrolysate samples using highperformance liquid chromatography (HPLC). Four monoterpenes were tested for their potential to extract furfural (FF) with simultaneous DES formation. An optimization process of the most important parameters affecting the extraction process and DES formation (Thymol:FF) was conducted using the Central Composite Design (CCD) model. A temperature of 40 °C, pH of 7, mHBD:mHYD ratio of 2:1, and time of 50 min were selected as the optimal conditions. These results indicate the high efficiency of FF removal from hydrolysates (92.1 - 94.6 %) in a onestep process. Meanwhile, the structural properties of the formed DES measured by Fouriertransform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance spectroscopy (NMR) differed only slightly from those of the DES composed of pure substances (Furfural and Thymol).
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Authors and Affiliations

Patrycja Makoś-Chełstowska
1
ORCID: ORCID
Edyta Słupek
1
ORCID: ORCID
Karolina Kucharska
1
ORCID: ORCID
Jacek Gębicki
1
ORCID: ORCID

  1. Gdansk University of Technology, Faculty of Chemistry, Department of Process Engineeringand Chemical Technology, 80-233 Gdansk, Poland
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Abstract

In the present study, the enrichment and isolation of textile effluent decolorizing bacteria were carried out in wheat bran (WB) medium. The isolated bacterium Providencia rettgeri strain HSL1 was then tested for decolorization of textile effluent in consortium with a dyestuff degrading fungus Aspergillus ochraceus NCIM 1146. Decolorization study suggests that A. ochraceus NCIM 1146 and P. rettgeri strain HSL1 alone re moves only 6 and 32% of textile effluent American Dye Manufacturing Institute respectively in 30 h at 30 ±0.2°C of microaerophilic incubation, while the fungal-bacterial consortium does 92% ADMI removal within the same time period. The fungal-bacterial consortium exhibited enhanced decolorization rate due to the induction in activities of catalytic enzymes laccase (196%), lignin peroxidase (77%), azoreductase (80%) and NADH-DCIP reductase (84%). The HPLC analysis confirmed the biodegradation of textile effluent into various metabolites. Detoxification studies of textile effluent before and after treatment with fungal-bacterial consortium revealed reduced toxicity of degradation metabolites. The efficient degradation and detoxification by fungal-bacterial consortium pre-grown in agricultural based medium thus suggest a promising approach in designing low-cost treatment technologies for textile effluent.

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Authors and Affiliations

Harshad Lade
Avinash Kadam
Diby Paul
Sanjay Govindwar

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