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Abstract

To test the potential harmfulness of soils fertilized with sludge-based products to plant organisms, a biotest method using the physiological/biochemical reaction of the organisms to assess their toxicity was chosen. This paper presents the results of a preliminary ecotoxicological study of different products: a sludge-based fertilizer, a plant growth promoter, and a reclamation blend. The study was conducted using Sinapis alba L., a plant used in agriculture for intercropping and recommended for toxicological testing. Toxicity tests were performed in a gradient of concentrations of the indicated products (2.5%, 5%, and 10%). For comparison purposes, a trial containing a commercial fertilizer was used alongside the control soil (without additives). The fertilizer and the crop support agent were of low toxicity, but data analysis indicated toxicity of the so-called reclamation blend, which contained heavy metals among other things. The test products showed an increase in toxicity with the increasing dose used. This research represents an important step in assessing the usefulness of products created from sewage sludge and may help overcome the „psychological barrier” that prevents potential investors from investing capital that would allow production to spread.
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Authors and Affiliations

Anna Borgulat
1
Aleksandra Zagórska
1
Marcin Głodniok
1

  1. Central Mining Institute, Department of Water Protection, Katowice, Poland
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Abstract

Multiple anthropogenic agents have turned Lake Maracaibo into a hypereutrophic environment. Heavy metals resulting from the steel and oil industry augment pollution in the lake. There is a lack of research on the ecotoxicological effect of heavy metals in protozoa. To evaluate the ecotoxicological effect of Cr3+, Cr6+, Cd2+, Pb2+ and Ni2+ on free-living ciliated protozoa and to identify suitable ciliated protozoa candidates for bioindicators of water quality; we estimated the lethal concentration for 50% of the protozoa population (LC50) in samples from two stations (S1: narrow of Maracaibo and S2: South of the lake) using ecotoxicological tests in the Sedgewick–Rafter chamber and Probit analysis. The general toxicity patterns obtained for S1 protozoa (Euplotes sp. and Oxytricha sp.) were Cr3+ > Cd2+ > Pb2+ > Cr6+ > Ni2+; and those corresponding to S2 (Coleps sp. and Chilodonella sp.) were Cr6+ > Cr3+ > Cd2+ > Pb2+ > Ni2+. We found statistically significant difference (p < 0.05) in the LC50 of protozoa exposed to Cr3+, Cr6+, Ni2+ and Pb2+ when comparing the two sampling stations. The differences observed in toxicity patterns are probably the result of various kinds of protozoa adaptation, possibly induced by various sources, levels and incidents of exposure to heavy metals contamination of the protozoa studied and to the physicochemical conditions prevailing in the two selected stations. The levels of tolerance observed in the present study, allow us to infer that S2 ciliates are the most susceptible to the contaminants studied and can be used as possible microbiological indicators that provide early warning in studies of contamination by heavy metals in Lake Maracaibo.
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Authors and Affiliations

Fernando Luis Castro Echavez
1
Julio César Marín Leal
2

  1. University of La Guajira, Faculty of Engineering, Environmental Engineering Program, PICHIHÜEL Research group, km 5 vía a Maicao, 440002, Riohacha, Colombia
  2. University of Zulia, Faculty of Engineering, School of Civil Engineering, Department of Sanitary and Environmental Engineering (DISA), Maracaibo, Venezuela

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