Animals as a source of organs and tissues for xenotransplantation could become a backup solution for the growing shortage of human donors. The presence of human xenoreactive anti- bodies directed against Galα1,3Gal antigens on the cell surface of a pig donor triggers the activa- tion of the complement leading to a hyperacute reaction. The development of genetic engineer- ing techniques has enabled the modification of genomes by knocking in and/or knocking out genes. In this paper, we report the generation of modified pigs with ZFN mediated disruption of the GGTA1 gene encoding the enzyme responsible for synthesis of Galα1,3Gal antigens. ZFN plasmids designed to target the exon 9 region of the pig GGTA1 gene encoding the catalytic domain were injected into the pronuclei of fertilized egg cells. Among 107 piglets of the F0 gene- ration analyzed, one female with 9-nt deletion in exon 9 of the GGTA1 gene was found. 13 of 33 piglets of the F1 generation represented the +/- GGTA1 genotype and 2 of 13 F2 piglets repre- sented the -/- GGTA1 genotype. No changes in the animals’ behavior, phenotype or karyotype were observed. Analysis confirmed heredity of the trait in all animals. A complex functional analysis of the modified animals, including flow cytometry, human serum cytotoxicity test and immunohistochemical detection, was performed to estimate the phenotype effect of genetic modification and this indicated an efficient GGTA1 knock-out in modified pigs.
On November 2, 2018, an outstanding Polish medievalist Jerzy Lesław Wyrozumski died in Kraków; he was born on March 7, 1930 in Trembowla (now Ukraine). He graduated in 1955 with a degree in history at the Jagiellonian University. He wrote his master's thesis and doctoral dissertation under the supervision of Roman Grodecki. In 1981 he received the title of professor; he was dean of the Faculty of Philosophy and History in the years 1981–1987, and from 1987 to 1990 he was the prorector of the Jagiellonian University. He published over 600 scholarly books, articles and reviews.
Intellectual and spiritual formation of Joseph Ratzinger - Pope Benedict XVI - requires talk about the process of his development. The article refers to the most overlooked phase of the young Ratzinger, who moves from the position of a progres-sive and sometimes irresponsible theorizing academic theologian to the position of a thinker grounded in the community of faith, becoming the guardian and teacher of Christian doctrine in line with the spirit and tradition of the Church. A lot of light on the change in the attitude of the German professor sheds the preparatory phase to the Second Vatican Council and its debates, as well as the unpleasant experience of the student revolt in 1968, which finds our professor in Tübingen. And in this way the liberal German theologian grows into to an outstanding Catholic theologian of the universal Church.
Article published in Science, 2012 by Jennifer A. Doudna, Emmanuelle Charpentier and their team presented a novel tool named as CRISPR/Cas9. The original CRISPR/Cas9 tool and the whole system developed from it since then allow making precise changes in the nucleotide sequence in the defined locus of the genome. The article presents the already known as well the potential future applications of the system for improvement of cultivated plants. The separate section is devoted to present the background of the Court of Justice decision C-528/16. Discussed are the far reaching negative consequences of this, based not on the merit decision, for the future of European green biotechnology.
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