Abstract
The aim of this study was to investigate the effect of different extenders on the post-thaw (PT) quality of sperm originating from the sperm-rich fraction (SRF) and post-sperm-rich fraction (PSRF) of boar ejaculate. Motility and velocity parameters, analyzed using a computer-assisted semen analysis (CASA) system, and membrane integrity parameters were markedly higher in frozen-thawed (FT) spermatozoa of the SRF in both the Belstville Thawing Solution (BTS) and Androhep Plus (AHP) extenders, irrespective of the post-thaw (PT) storage time. Furthermore, reduced cryo-survival was more marked in FT spermatozoa of the PSRF in both extenders following storage for 60 min. It was found that the SRF-stored samples in the AHP extender for 60 min exhibited significantly higher percentages of spermatozoa with total motility, mitochondrial function and acrosome integrity than those stored in the BTS extender. The findings of this study confirm that components of the ejaculate fractions and extender have varying effects on the cryo-survival of boar spermatozoa.
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Bibliography
1. Aquila S, Giordano F, Guido C, Rago V, Carpino A (2011) Nitric oxide involvement in the acrosome reaction triggered by leptin in pig sperm. Reprod Biol Endocrinol 9: 133.
2. Fraser L, Strzeżek J (2007) Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar sper-matozoa following freezing-thawing. Anim Reprod Sci 99: 317-329.
3. Kaeoket K, Chanapai P, Junchiyaphoom P, Chanapiwat P (2011) The effect of using long term and short term extenders during cooling process on the quality of frozen boar semen. Thai J Vet Med 41: 283-288.
4. Rodríguez-Martínez H, Martínez EA, Calvete JJ, Peña Vega FJ, Roca J (2021) Seminal plasma: relevant for fertility? Int J Mol Sci 22: 4368.
5. Rodríguez-Martínez H, Saravia F, Wallgren M, Roca J, Peña FJ (2008) Influence of seminal plasma on the kinematics of boar sperma-tozoa during freezing. Theriogenology 70: 1242-1250.
6. Saravia F, Wallgren M, Johannisson A, Calvete JJ, Sanz L, Pena FJ, Roca J, Rodríguez-Martínez H (2009) Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks. Theriogenology 71: 662-675.
7. Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
8. Wasilewska K, Fraser L (2017) Boar variability in sperm cryo-tolerance after cooling of semen in different longterm extenders at various temperatures. Anim Reprod Sci 185: 161-173.
9. Wasilewska-Sakowska K, Zasiadczyk Ł, Fraser L (2019) Effect of fractionated seminal plasma on sperm characteristics following cryo-preservation of boar semen. Ann Anim Sci 19: 695-712.
10. Weitze KF (2014) Benefits of AndrohepPlus and AndrostarPlus long-term extenders for boar semen. (Minitüb Gmbh) Technical Report 5: 1-6.
11. Yeste M (2016) Sperm cryopreservation update: cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenol-ogy 85: 47-64.
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