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Abstract

Water extractsfrom selected Geraniaceae plants, to which paraffin oil was added as adjuvant, were tested. It was observed that the plant extracts researched limited Colorado potato beetle feeding and development and adding adjuvant increased the effects. The highest antifeedant activity towards Colorado potato beetlesand their larvae wasobs erved in extractsobtained from Pelargonium × hortorum Bailey and Geranium pusillum L. The extract from Pelargonium × hortorum Bailey added to food showed a negative effect on the development of female reproductive organs and embryo development and showed the highest effectiveness in field conditions.

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Authors and Affiliations

Robert Lamparski
Maria Wawrzyniak
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Abstract

Solanaceae plants have strong allelopathic potential, and therefore the action is confirmed through: a) bioassays with liquid or various solvent extracts and residues, b) fractionation, identification, and quantification of causative allelochemicals. Most assessments of allelopathy involve bioassays of plant or soil extracts, leachates, fractions, and residues which support seed germination and seedling growth in laboratory and greenhouse experiments. Plant growth is also stimulated below the allelopathic threshold, however severe growth reductions may be observed above the threshold concentration depending on the sensitivity of the receiving species. Generally, seedling growth is more sensitive than germination, particularly root growth. Some approaches showed that field soil collected beneath donor plants significantly reduced or somewhat promoted the growth of the recipients plants. Petri dish bioassays with aqueous extracts of different parts of donor plants showed considerable phytotoxic activities in a concentration-dependent manner with leaf aqueous extracts being most dominant. Delayed seed germination and slow root growth attributable to the extracts may be baffled with diffusion effects on the rate of imbibition, delayed initiation of germination, and particularly cell elongation; the main factor that is responsible for affecting root growth before and after the tip penetrates the testa. Light and electron microscopy extract analysis at the ultrastructural level are correctly investigated. Several Solanaceae plants have allelopathic potential, and therefore the activities, kinds and quantity of allelopathic compounds differ depending on the plant species. The incorporation of allelopathic substances into agricultural management might scale back the development of pesticides and reduce environmental deterioration.
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Authors and Affiliations

Mushtaq Waseem
Siddiqui Mohammad Badruzzaman
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Abstract

The insecticidal and antifeedant activity of extracts derived from different plants of the Liguidamber orientalis Mill. (Hamamelidaceae), Tanacetum vulgare L. (Compositae), Achillea coarctata Willd. (Compositae), Buxus sempervirens L. (Buxaceae), Diospyros kaki L. (Ebenaceae), Arum italicum Mill. (Araceae), Achilea biebersteinii Willd. (Compositae), Origanum vulgare L. (Labiatae), Hypericum androsaemum L. (Hypericaceae) and Ocimum basilicum L. (Labiatae). are reported. The 70% alcohol extracts of plants were tested for toxicity against the 3–4th instar larvae of the Yponomeuta malinellus Zell. (Lepidoptera.: Yponomeutidae). Antifeedant activity of the extracts was assessed through tests conducted on the larvae of Y. malinellus by the feeding protection bioassay. In tests carried out on the larvae of Y. malinellus, L. orientalis, O. basilicum and A. coarctata extracts showed high toxicity within 48 hour LC50’s of 75, 75 and 65% respectively. The toxicity effects of the other extracts were determined as 60, 50, 50, 50, 45, 45 and 40% within the same period, respectively. No mortality was noticed in control groups. Alcohol extract from L. orientalis, T. vulgare and B. sempervirens showed high antifeedant activity (80.90, 46.12) on the larvae of Y. malinellus. In addition to both T. vulgare and L. orientalis extracts caused decrease consumption of food per 1 mg of larvae body weight decrease showed high –8.465, –0.845, mg respectively. The highest consumption (557.6 mg) was observed with alcohol extract from, D. kaki whereas the minimum one was using alcohol extract from L. orientali. The other tested extracts showed similar activity.

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Authors and Affiliations

Ömer Ertürk
Vedat Şekeroğlu
Ahmet Koç Yavuz Kalkan
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Abstract

The antifungal effect of twenty powdered spice plants and their extracts at concentrations of 2, 4, 8 and 1, 3, 6%, respectively was evaluated in relation to the radial mycelial growth of various soilborne fungi causing damping-off disease. The spice powder or extract were added to the culture medium PDA to obtain the proposed concentrations. Concentration of 8% of powdered spices and 6% of their extracts were able to cause complete growth inhibition of major tested fungi. High significant inhibitory effect on radial fungal growth was observed for different concentrations of carnation (Dianthus caryophyllus), cinnamon (Cinnamomum burmannil), garlic (Allium sativum) and thyme (Thymus vulgaris). Meanwhile, fennel (Foeniculum vulgare), marjoram (Origanum majorana) and chamomile (Matricaria hamomilla) showed a low inhibitory effect on tested fungi. Moderate inhibitory effect was observed with the other tested spices. In the greenhouse, efficacy of spice plants as powder or their extracts in addition to the fungicide Rizolex-T used as seed dressings against faba bean damping-off incidence was evaluated in pot experiment using soil artificially infested with the disease agents (Fusarium solani and Rhizoctonia solani). Spice extracts showed superior reducing effect on damping-off disease incidence at pre-emergence growth stage to that of powder treatments and Rizolex-T as well, while an opposite effect was observed at post-emergence growth stage. Carnation and cinnamon spices showed the highest protecting effect against disease incidence when applied as powder or extracts. It is interesting to note that spice plants as powder or extracts gave a similar effect to the fungicide Rhizolex-T in reducing damping-off incidence either at pre- or post-emergence stages of faba bean growth. Promising applicable technique could be suggested in the light of the results obtained. The use of spice plants as powder or extract for seed dressing might be considered as safe, cheep and easily applied method for controlling soilborne plant pathogens considering the avoidance of environmental pollution and the side effect of pesticide application.

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Authors and Affiliations

Nehal S. El-Mougy
Mokhtar M. Abdel-Kader
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Abstract

The effect of crude extracts of neem (Azadirachta indica) leaf, neem seed and garlic (Allium sativum) at concentrations ranging from 5% to 30% of the material in 100 ml of Potato Dextrose Agar on mycelial growth of Fusarium oxysporum f. sp. lycopersici was assessed. All the extracts inhibited mycellial growth at various levels. Dry neem seed extract gavel 100% inhibition of mycelial growth. Fresh neem leaf extract reduced mycelial growth with increasing concentration while in garlic there were no differences in growth inhibition among the various concentrations used. However garlic extracts decreased sporulation with increasing concentration and cultures grown on extract amended agar plates remained viable.

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Authors and Affiliations

Ogechi N. Agbenin
P.S. Marley
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Abstract

Elevated content or heavy metals in soils is characteristic of the Dąbrowa Górnicza region. The highest concentrations of lead. cadmium and zinc exceed herc 650, 15, and 1000 mg/kg of soil, respectively. Samples of soi I from selected sites underwent the speciation analysis with the use of the Tessier method. modified according to Kersten and Forstner. Results of the investigations proved the highest concentrations or these metals in the area of Trzebiesławice. They occur here in the strongly bound forms and, mainly, their occurrence is related to presence of limestone rocks. The greatest amounts of these metals in easily assimilable to plant forms occur within the area of the town of Dąbrowa Górnicza. The most probable source ot· most of these heavy metals in soils are here contaminants emitted by the industry, mainly by the metallurgy. In the vicinity of the town of Błędów, mainly sandy soils occur, characteristic or which is low content of considered metals. Weak sorption capacities of these soils account for relatively good cxtractability of the three metals. In soils from the Lęka area, strong binding of these metals was confirmed. Occurrence or cadmium should be of special attention because this metal occurs as built in the crystal lattice of minerals.
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Authors and Affiliations

Bronisław Wyżgolik
Stanislaw Karweta
Ewa Surowiec
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Abstract

Natural resources and the extractive industries play a central role in the economy of developing countries and the lives of nearly half of the world’s population. The increasing demand for oil, gas, and mineral resources has led some countries to prioritize the extractive industries; yet, there is growing empirical evidence that in some cases governments have neglected other sectors of the economy, making them highly dependent and vulnerable to volatile commodity prices. Latin American countries face the challenge of changing their model of primary-export specialization and move away from their dependence on natural resource-intensive exports in order to avoid being vulnerable to commodity cycles. In this context, given the limited literature available on measuring the dependence on the extraction of oil, gas and minerals of the Ecuadorian economy, the objective of this article is to twofold. First, to provide a snapshot of the historical and current situation of Ecuador’s natural resource dependence. Second, to estimate the Extractives Dependence Index (EDI) scores for Ecuador for the years 2003 to 2017. The EDI is a generally accepted method for measuring a country’s aggregate dependence on natural and mineral resources. Based on the EDI scores obtained, we analyze the variation of this indicator and investigate the effect of extractives dependence on the Ecuadorian economy. Results show that despite the government’s significant efforts to diversify Ecuador’s economy, the country has a persistent dependence on the extractive sector.

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Authors and Affiliations

Pablo Benalcazar
Luis Felipe Orozco
Jacek Kamiński
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Abstract

For much of the last two decades, the Central and East European (CEE) economies have experienced a deep structural reform, moving away from a socialist economic system towards a market economy. The political situation of the second half of the 20th century had a significant impact on the economic development and competitiveness of these transition countries, when compared with their Western European counterparts. A vast number of studies have been conducted to analyze the structural changes required for resource-dependent economies to achieve long-term development and to understand the synergies between commodities and diversification. Yet, the dynamics of resource extraction and the resource dependence of regions that have experienced periods of sustained levels of growth have largely been overlooked, especially the Central and Eastern European region. In this context, this article presents an analysis of the level of resource dependence of six countries which joined the European Union between 2004 and 2007. Using data spanning from the year 2000 to 2017, we calculate the Extractives Dependence Index (EDI) of six former Soviet satellite nations and one former Soviet state. Our results indicate that the commodity structure of trade in the six countries which joined the European Union has changed considerably. These countries have reduced their economic dependence on extractive resources by developing their high value-added and technology-intensive sectors. Our findings also reveal that Poland experienced the highest decrease in EDI scores among the six CEE countries.

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Authors and Affiliations

Marcin Malec
ORCID: ORCID
Pablo Benalcazar
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Abstract

The efficacy of some plant extracts on the control of potato tuber soft rot caused by Erwinia carotovora ssp. carotovora was evaluated in the laboratory conditions. Neem leaf and seed aqueous extracts significantly reduced the incidence and severity of tuber soft rot, while ironweed and Siamese cassia aqueous leaf extracts gave moderate control of the disease. Tuber treatment with river redgum aqueous leaf extract did not control tuber soft rot.

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Authors and Affiliations

Bulus S. Bdliya
Bashir Dahiru
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Abstract

Several species of Solanum produce secondary metabolites with antimicrobial activity. In

the present study, the inhibitory activity of Solanum chrysotrichum, S. erianthum, S. torvum

and S. rostratum against phytopathogenic Curvularia lunata was determined. Methanol extracts

from roots, stems, leaves and fruits were evaluated by the method of mycelial inhibition

on agar and the minimum inhibitory concentration (MIC) was determined on a liquid

medium. To increase the antimicrobial activity, the combined activity of the most active

extracts for each phytopathogen was also determined (a combination of intra and interspecies

extracts). The results showed that 12 of the 16 methanolic extracts of Solanum species

had antifungal effects against C. lunata. The extracts of S. rostratum and S. erianthum

developed the highest activity (~80% inhibition and 28.4 MIC μg . ml–1), even, equal to or

greater than, the reference fungicide. The mixture of the active extracts of S. chrysotrichum

and S. torvum increased their activity. Various extracts affected the macro and microscopic

morphology and most of them reduced the number of conidia of the fungus. This resulted

in the capacity to control the vegetative growth and reproduction of C. lunata, the causal

fungus of corn leaf spot disease.

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Authors and Affiliations

Zuleima Guadalupe Hernández-Rodríguez
Christian Anabi Riley-Saldaña
Alma Rosa González-Esquinca
Marisol Castro-Moreno
Iván de-la-Cruz-Chacón
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Abstract

The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.

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Authors and Affiliations

Simin Emamzadeh Yazdi
Julia Mulabisana
Gerhard Prinsloo
Michele Cloete
Quenton Kritzinger
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Abstract

Coal mining activities carried out for 200 years in Upper Silesia have had a negative effect on buildings. T his impact is in all cases related with continuous deformations of the surface and in certain cases with discontinuous deformations (mostly cave-ins), changes in water relations and mining tremors. T he paper presents an evaluation of the impact of a mining activity on a building situated in the Upper Silesian Coal Basin. T he building was affected by continuous deformations and mining tremors. Calculations were made of the values of deformation rates by means of Budryk–Knothe’s theory, which were partly verified on the basis of the results from geodetic measurements. An analysis of the velocity and acceleration of basement vibrations caused by mining-induced tremors was also conducted. T he conclusions included a high consistency between the results obtained on the basis of calculations and the values obtained by means of PGA and PGV measurements. In the case of tremors with the highest energy in the hipocentrum, there an empirical formula allowing for calculation of PGA value in given geological and mining conditions was also proposed. T he application range of the formula mentioned above is obviously limited only to the conditions in consideration. The presented conclusions indicate that at present, sufficiently precise methods, allowing for calculations for practical purposes, not only of deformation indices’ values, but also of PGV and PGA values, presently exist.

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Authors and Affiliations

Piotr Strzałkowski
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Abstract

The aim of the study was fractionation of Zn, Cu, Ni, Pb in the bottom sediments of two small reservoirs: at Krempna on the Wisłoka River and at Zcslwicc on the Dlubnia River. The partitioning of metals for various fractions was performed with the use of Tessier's sequential chemical extraction method. All together five metal fractions were distinguished: exchangeable metals (fraction I), metals bound to carbonates (fraction II), metals bound to hydrate Fe-Mn oxides (fraction III), metals bound to organic matter (fraction IV), and metals bound to minerals (fraction V). The largest quantities of metals were bound with fraction V, the smallest occurred in the forms most easily available for living organisms, in faction I. Proportions ofZn, Ni and Pb in the exchangeable fraction were about 1%. The amounts of metals bound with fraction II were also relatively low, except lor Zn in bottom sediment at the Zeslawice Reservoir. In this bottom sediment the share of Zn bound to carbonates was 33%. Medium metal quantities were associated with hydrate Fe-Mn oxides (fraction III) and with organic matter (fraction IV). Relatively high proportion ofmetals in fraction V and trace amounts ofmetals in fraction I as well as alkaline and neutral reaction of the sediments may prove a potentially low hazard of the metal release in the case of chemical changes in the reservoirs.
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Authors and Affiliations

Marek Madeyski
Marek Tarnawski
Czesława Jasiewicz
Agnieszka Baran
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Abstract

Stevia rebaudiana Bertoni which has gained industrial and scientific interests is a suitable nutritional alternative to sucrose as a sweetener. Recently, there have been studies which show the extraction of this phytochemical substance from stevia leaves and purification methods by several alcohols and chromatographic methods. However, these methods are not cost-effective. Therefore, an attempt was made to extract and purify ST using inexpensive, scalable and simple techniques where different steps like extraction, electrocoagulation, ion exchange, activated charcoal, vacuum evaporation and butanol wash were used as purification steps. The present study established a new improved technology of extraction of ST from stevia leaves using water as a solvent followed by various purification steps. 496 mg of Stevioside extracted in the form of crystals was obtained from 100 g of leaves which is 10 times more than the reported yield of 54 mg from 100 g stevia leaves in literature. This methodology can be scaled up at the industry level for future large production to meet the huge demand for natural sweeteners.
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Authors and Affiliations

Jignesh Joshi
1 2
Alok Gautam
3 2
Shina Gautam
3 2

  1. Chemical Engineering Dept, Government Engineering College, Valsad, Gujarat, India-382424
  2. Gujarat Technological University, Chandkheda, Ahmedabad, 382424 Gujarat, India
  3. Chemical Engineering Dept, Shroff S. R. Rotary Institute Of Chemical Technology, Bharuch 393135, Gujarat, India
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Abstract

Antibiotics are used for postsurgical wound healing purposes but unfortunately, resistance against them demands some alternatives for quick recovery. Sepsis of wounds is a challenge for medical as well as veterinary professionals. Nanoparticles have significant advantages in wound treatment and drug resistance reversal. This study was conducted to appreciate emerging alternates of antibiotics like zinc oxide nanoparticles and plant extracts in topical application. Zinc oxide is considered a good wound healer and its nanoparticles are easy to access. So, the efficacies of zinc oxide nanoparticles and sweet flag plant extract ointments were tested to compare modern and traditional therapeutics as sweet flag is considered a pure medicinal plant. Rabbits were selected for this study due to the healing properties of their skin. Wounds were inflicted on the thoracolumbar region and treated for 29 days post-surgically daily with normal saline and the ointment of zinc oxide nanoparticles and sweet flag extract ointment, prepared in a hydrophilic solvent. Wound shrinkage was observed daily and histopathological analysis was made and results were compared. Zinc oxide nanoparticles ointment showed the most satisfactory results for every parameter included in the study. No side effects of its topical application were observed. Healing was normal without any complications. The preparations of zinc oxide nanoparticles may help in the era of antibiotic resistance as topical drugs in the future.
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Authors and Affiliations

A. Abbas
1 2
S.A. Muhammad
3
A. Ashar
4
S.A. Mehfooz
2
A. Rauf
3
M. Bakhsh
3
T. Nadeem
5
H. Fu
1

  1. Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China
  2. Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38000, Pakistan
  3. University of Veterinary and Animal Sciences, Lahore, CVAS Jhang 35200, Pakistan
  4. Wilson College of Textiles, North Carolina State University, North Carolina, USA
  5. University of Veterinary and Animal Sciences, Lahore, Para Veterinary Institute (PVI), Karor 31100, Layyah, Pakistan
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Abstract

Herpesviruses (HV) are pathogens causing infections in humans and animals worldwide. Since it shares many common features with other HV, bovine HV type 1 (BoHV-1) was selected as a model to test the anti-herpesviral activity of medicinal plants.

Fifteen plants were chosen in this study for their medical, antibacterial and antiviral properties. The aim was to investigate ethanolic extracts from the selected medicinal plants for anti-BoHV-1 activity. The virucidal activities were evaluated by comparing the effect of noncytotoxic concentrations of extracts on BoHV-1 strain 1640 replication in Madin-Darby bovine kidney (MDBK) cells. Virucidal activity was determined by means of virus titration after exposure to the extracts. The extract of Desmodium canadense was found to be the most effective virucide – the 50% tissue culture infective dose (TCID50) after exposure was 3.75 log10 and the virus reduction factor was ≥5.0±0.25 log10. The extract of D. canadense was therefore chosen for further studies. Virus yield reduction assays showed that D. canadense extract had time-dependent and dose-dependent effects. It effectively reduced virus titre from 8.33 log10 to 4.67 log10 (p<0.01). The virucidal activity was also confirmed by real-time polymerase chain reaction (real-time PCR), where the number of threshold cycles (Ct) was inversely proportional to the virus titre in TCID50 The virucidal activity was also confirmed by real-time polymerase chain reaction (real-time PCR). This method showed that the number of threshold cycles (Ct) was inversely proportional to the virus titre (direct correlation with exposure time R=0.9321). The extract of D. canadense showed a high virus reduction capacity. In future, such active substances should be identified for the development of effective antivirals.

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Authors and Affiliations

R. Lelešius
P. Girdauskaitė
A. Karpovaitė
R. Mickienė
T. Drevinskas
N. Tiso
O. Ragažinskienė
L. Kubilienė
A. Maruška
A. Šalomskas
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Abstract

Despite many phytochemical and pharmacological investigations, to date, there are no reports concerning the antibabesial activity of extracts of A. millefolium against B. canis. This study was aimed at investigating the biological activities of A. millefolium against the Babesia canis parasite and to identify its chemical ingredients. The water (WE), ethanol (EE) and hexane/acetone (H/AE) extracts of plant aerial parts were screened for total phenolic content (TPC), total flavo- noid compound (TFC), DPPH free radical-scavenging activity and its antibabesial activity assay. In this study, imidocarb diproprionate was used as a positive control. The H/AE and EE extracts were analysed using gas chromatography–mass spectroscopy (GC–MS).

In the EE extract, the main compounds were 17.64% methyl octadec-9-ynoate, 16.68% stigmast-5-en-3-ol(3α,24S) and 15.17% hexadecanoic acid. In the H/AE extract, the main com- pounds were 34.55% 11-decyldocosane, 14.31% N-tetratetracontane, 8.22% β-caryophyllene, and 7.69% N-nonacosane. Extract of EE contained the highest content of phenolics followed by H/AE and WE. The concentration of flavonoids in EE, H/AE and WE extracts showed that TFC was higher in the EE samples followed by H/AE and WE. The antioxidant activities were highest for AA, followed by EE, WE and H/AE. The antibabesial assay showed that the WE, EE and H/AE extracts of A. millefolium were antagonistic to B. canis. At a 2 mg/mL concentration, it showed 58.7% (± 4.7%), 62.3% (± 5.5%) and 49.3% (± 5.1%) inhibitory rate in an antibabesial assay, respectively.

Considering these results, the present findings suggest that A. millefolium extracts may be a potential therapeutic agent and that additional studies including in vivo experiments are essential.

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Authors and Affiliations

L. Guz
Ł. Adaszek
J. Wawrzykowski
J. Ziętek
S. Winiarczyk
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Abstract

Individual identification of similar communication emitters in the complex electromagnetic environment has great research value and significance in both military and civilian fields. In this paper, a feature extraction method called HVG-NTE is proposed based on the idea of system nonlinearity. The shape of the degree distribution, based on the extraction of HVG degree distribution, is quantified with NTE to improve the anti-noise performance. Then XGBoost is used to build a classifier for communication emitter identification. Our method achieves better recognition performance than the state-of-the-art technology of the transient signal data set of radio stations with the same plant, batch, and model, and is suitable for a small sample size.
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Authors and Affiliations

Ke Li
1 2 3
ORCID: ORCID
Wei Ge
1 2
ORCID: ORCID
Xiaoya Yang
1 2
Zhengrong Xu
1

  1. School of Information and Computer, Anhui Agricultural University, Hefei, Anhui, 230036, China
  2. Anhui Provincial Engineering Laboratory for Beidou Precision Agriculture Information, Anhui Agricultural University, Hefei, Anhui, 230036, China
  3. Key Laboratory of Specialty Fiber Optics and Optical Access Networks, Shanghai University, Shanghai, 200072, China
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Abstract

The market value of essential oils is steadily increasing every year. They are mainly used as aromas and preservatives in food and cosmetics industries. The content and yield of the obtained extracts change across the seasons of the year. The knowledge of these differences is important for essential oils industry to gain the best quality and quantity of products. In this study Thuja occidentalis L. (Eng. northern white cedar) extract was obtained from leaves by maceration in dichloromethane. Plant material was collected during each season of the year and the finished products were thoroughly examined through GC-MS analysis. The seasonal variations of volatile compound composition showed that the most adequate period to obtain T. occidentalis extract on an industrial scale is spring. This is due to the best efficiency (almost 10%) and the highest content of monoterpenes (67%).
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Authors and Affiliations

Marek Chajduk
1
Marek Gołębiowski
1

  1. Laboratory of Analysis of Natural Compounds, Department of Environmental Analysis, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308 Gdańsk, Poland
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Abstract

The use of foam fractionation followed by aqueous two-phase extraction has emerged as a potential alternative to traditional liquid chromatography, hitherto irreplaceable in the purification of phycobiliproteins. The crude extracts of C-phycocyanin and allophycocyanin were obtained after Thermosynechococcus PCC 6715 biomass disintegration. The FF process with air flow of 2.4 L·h -1 resulted in purification factors up to 1.47 and partitioning coefficients of about 39, and did not require the addition of surfactants. A temperature of 35˚C allowed for the highest partitioning coefficient of 67.6 and yield of 76%; however, the purity of C-PC in condensate at this temperature was lower than at 25˚C. ATPE was tested in 20 different systems consisting of polyethylene glycol and phosphate or citrate salts, of which PEG1500-citrate gave the highest purification factor value of 2.31. Conversely, a partitioning coefficient of 2416 and 1094 were obtained for the PEG1500-phosphate and PEG3000-phosphate systems, respectively. Interestingly, the use of FF condensate in subsequent ATPE step resulted, for the first time, in the separation of the polymer phase into two fractions, one contained C-phycocyanin and the other allophycocyanin. It can be concluded that the use of a two-step system of FF and ATPE is a viable way to separate phycobiliproteins.
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Authors and Affiliations

Anna Antecka
1
ORCID: ORCID
Rafał Szeląg
1
Stanisław Ledakowicz
1
ORCID: ORCID

  1. Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wolczanska 213, 93-005 Lodz, Poland
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Abstract

The total content of lead in soil from the battery plant site was determined and a speciation analysis of this element was carried out using Rudd's method of sequential extraction. It was found out that lead is present in soil samples mostly in a low mobility organically bound form, which under certain conditions can, however, be absorbed by plants, and in a moderately mobile carbonate form. Lead was extracted from soil using sodium salt of ethylenediaminetetraacetic acid and sodium hydroxide solution and tests were also conducted to separate it from leaching solutions electrochemically. Extraction with Na2 -EDTA solution makes it possible to remove 86% of lead from soil, but the solution shows much lower lead extraction power when used for renewed leaching after having been electrochemically freed of lead. Extraction with NaOH solution gives worse results, namely only 70% of lead can be removed from soil, but the solution after having been electrochemically freed of Pb shows higher Pb leaching power when used for renewed leaching than in the case of Na2 - EDTA solution.
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Authors and Affiliations

Teofil Korolewicz
Marian Turek
ORCID: ORCID
Jerzy Ciba
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Abstract

Recently, since the demand of rare earth permanent magnet for high temperature applications such as an electric motor has increased, dysprosium (Dy), a heavy rare earth element, is becoming important due to severe bias in its production. To fulfill the increasing need of Dy, recycling offers as a promising alternative. In recycling of rare earths, Hydro-metallurgical extraction method is mainly used however it has adverse environmental effects. Liquid metal extraction on the other hand, is an eco-friendly and simple method as far as the reduction of rare earth metal oxide is concerned. Therefore, liquid metal extraction was studied in this research as an alternative to the hydro-metallurgical recycling method. Magnesium (Mg) is selected as solvent metal because it doesn’t form intermetallic compounds with Fe, B and has a low melting and low boiling point. Extraction behavior of Dy in (Nd,Dy)-Fe-B magnet is observed and effect of Mg ratio on extraction of Dy is confirmed.

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Authors and Affiliations

Sangmin Park
Sun-Woo Nam
ORCID: ORCID
Ju-Young Cho
ORCID: ORCID
Sang-Hoon Lee
ORCID: ORCID
Seung-Keun Hyun
Taek-Soo Kim
ORCID: ORCID
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Abstract

Tannic acid or tannin, type of phenolic compound contains in kenaf bast fibre. Conventional extraction has certain limitations in terms of time, energy, and solvent consumption. Ultrasound assisted extraction (UAE) can extract bioactive components in shorter time, low temperature, with lesser energy and solvent requirement. UAE as alternative extraction technique is better equipped to retain the functionality of the bioactive compounds. In this study, the conditions for ultrasound assisted extraction (UAE) of tannic acid from kenaf bast fibre by assessing the effect of sonication time and different duty cycles were optimized. The use of ultrasound to extract tannic acid from kenaf bast fiber was evaluated. Ultrasound-assisted extraction (UAE) was carried out using ethanol as solvent to intensify the extraction efficacy. Phytochemical screening was conducted to identify the presence of tannic acid in extracts. The extracts then were analyzed using High-Performance Liquid Chromatography (HPLC) and Scanning Electron Microscopy (SEM). It was found that 0.2429 mg/mL of tannic acid was obtained under the extraction conditions of extraction temperature of 40℃, sonication time of 20 minutes and duty cycle of 50%. From SEM analysis, it was found that the raw sample demonstrated rough surface and no porous but kenaf bast fibre display smoother surface with less impurities and few pores appeared after the extraction process using UAE. These results indicate that ultrasound-assisted extraction is an efficient method for extracting tannic acid from kenaf bast fibre with the advantages of lower extraction time and higher extraction yield.
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Authors and Affiliations

M. Mohamad
1
ORCID: ORCID
H.A. Ibrahim
1
M.F.M. Nasir
2
N.A. Mohidem
3
ORCID: ORCID
N.F. Shoparwe
1
P.T. Teo
1
ORCID: ORCID
M.N. Masri
1
ORCID: ORCID
A.R. Irfan
4 5
ORCID: ORCID

  1. Universiti Malaysia Kelantan, Faculty of Bioengineering and Technology, Jeli Campus, Kelantan, Malaysia
  2. National Kenaf and Tobacco Board, Kubang Kerian, Kelantan, Malaysia
  3. Universiti Putra Malaysia, Faculty of Engineering Selangor, Department of Biological and Agricultural Engineering, Malaysia
  4. Universiti Malaysia Perlis, Faculty of Mechanical Engineering Technology, Perlis, Malaysia
  5. Universiti Malaysia Perlis, Center of Excellence Geopolymer and Green Technology (CEGeoGTech), Perlis, Malaysia
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Abstract

Underground mining extraction causes the displacement and changes of stress fields in the surrounding rock mass. The determination of the changes is extremely important when the mining activity takes place in the proximity of post-flotation tailing ponds, which may affect the stability of the tailing dams. The deterministic modeling based on principles of continuum mechanics with the use of numerical methods, e.g. finite element method (FEM) should be used in all problems of predicting rock mass displacements and changes of stress field, particularly in cases of complex geology and complex mining methods. The accuracy of FEM solutions depends mainly on the quality of geomechanical parameters of the geological strata. The parameters, e.g. young modulus of elasticity, may require verification through a comparison with measured surface deformations using geodetic methods. This paper presents application of FEM in predicting effects of underground mining on the surface displacements in the area of the KGHM safety pillar of the tailing pond of the OUOW Żelazny Most. The area has been affected by room and pillar mining with roof bending in the years 2008-2016 and will be further exposed to room-and-pillar extraction with hydraulic filling in the years 2017–2019.

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Authors and Affiliations

Ewa Warchała
Anna Szostak-Chrzanowski
Paweł Stefanek

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