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Abstract

Aim: The aim of this study was to analyze the effect of bovine follicular fluid on the survival, morphology and kinetic parameters of bovine thawed spermatozoa under laboratory conditions.

Materials and methods: The semen from 5 bulls of proven fertility was incubated in follicular and physiological fluid for 8 hours. During this time assessment using the CASA system was performed. At the beginning and the end of incubation process evaluation by flow cytometry was conducted.

Results: The results of the sperm motility assessment showed a significant decrease in the analyzed parameters both in the follicular and physiological fluid. A significant reduction in all parameters characterizing movement properties in the semen incubated in the follicular fluid was found. In the physiological fluid, a similar trend was demonstrated only for the following proper- ties: VAP, VSL, VCL, ALH, BCF. A significant difference was found for both fluids in: VCL (p=0.026), ALH (p=0.038) and LIN (p<0.001) at the beginning of incubation. The results of the plasma membrane integrity assessment showed a statistically significant increase in the percent- age of dying sperm at the 8th hour of the incubation in the follicular fluid. In the case of semen incubation in physiological fluid, a statistically significant decrease in the percentage of live non-damaged cells was found with a simultaneous increase in the subpopulation of undamaged dead cells.

Conclusions: Follicular fluid rapidly accelerates the capacitation process. The results of flow cytometry support the hypothesis concerning the ability of follicular fluid to prolong sperm sur- vival.

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Authors and Affiliations

J. Mrowiec
J. Twardoń
A. Bartoszewicz
W. Niżański
M. Ochota
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Abstract

We have developed an effective protocol for in vitro micropropagation in order to obtain large numbers of identical plants and another protocol for in vitro polyploidization of Ajuga reptans, based on the use of oryzalin. Two donor plants of A. reptans (AR 4, AR 7) were treated with 0, 1, 5, 10 μM oryzalin for 2 weeks. The analysis of the ploidy level of these plants was verified by flow cytometric analysis using the internal standardization method. The effects of polyploidization on growth as well as morphological and stomatal size were also measured. After in vitro polyploidization, some plants became tetraploids or octoploids. The most efficient conditions for inducing tetraploidy were the treatments with 10 μM oryzalin.

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Authors and Affiliations

Michaela Švécarová
Božena Navrátilová
Vladan Ondřej
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Abstract

This paper presents investigations on the removal of cyclohexane and ethanol from air in polyurethane- -packed biotrickling filters, inoculated with Candida albicans and Candida subhashii fungal species. Results on process performance together with flow cytometry analyses of the biofilm formed over packing elements are presented and discussed. The results indicate that the presence of ethanol enhances the removal efficiency of cyclohexane from air. This synergistic effect may be attributed to both co-metabolism of cyclohexane with ethanol as well as increased sorption efficiency of cyclohexane to mineral salt medium in the presence of ethanol. Maximum elimination capacities of 89 g m-3 h-1 and 36.7 g m-3 h-1 were noted for cyclohexane and ethanol, respectively, when a mixture of these compounds was treated in a biofilter inoculated with C. subhashii. Results of flow cytometry analyses after 100 days of biofiltration revealed that about 91% and 88% of cells in biofilm remained actively dividing, respectively for C. albicans and C. subhashii species, indicating their good condition and ability to utilize cyclohexane and ethanol as a carbon source.
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Bibliography

  1. Avalos, Ramirez, A., Jones, J.P. & Heitz, M. (2007). Biotrickling filtration of air contaminated with ethanol, Journal of Chemical Technology and Biotechnology, 82, pp. 149–157, https://doi.org/10.1002/jctb.1644.
  2. Cheng, Y., He, H., Yang, C., Zeng, G., Li, X., Chen, H. & Yu, G. (2016). Challenges and solutions for biofiltration of hydrophobic volatile organic compounds, Biotechnology Advances, 34, 1091–1102, https://doi.org/10.1016/j.biotechadv.2016.06.007
  3. Cheng, Y., Li, X., Liu, H., Yang, C., Wu, S., Du, C., Nie, L. & Zhong, Y. (2020). Effect of presence of hydrophilic volatile organic compounds on removal of hydrophobic n-hexane in biotrickling filters, Chemosphere 252, 126490, https://doi.org/10/1016/j.chemosphere.2020.126490.
  4. Cox, H.H.J., Sexton, T., Shareefdeen, Z.M. & Deshusses, M.A. (2001). Thermophilic Biotrickling Filtration of Ethanol Vapors, Environmental Science and Technology, 35, pp. 2612–2619, https://doi.org/10.1021/es001764h.
  5. Ferdowsi, M., Avalos, Ramirez, A., Jones, J.P. & Heitz, M. (2017). Elimination of mass transfer and kinetic limited organic pollutants in biofilters: A review, International Biodeterioration and Biodegradation, 119, pp. 336–348,https://doi.org/10.1016/j.ibiod.2016.10.015.
  6. Gospodarek, M., Rybarczyk, P., Szulczyński, B. & Gębicki, J. (2019). Comparative Evaluation of Selected Biological Methods for the Removal of Hydrophilic and Hydrophobic Odorous VOCs from Air, Processes 7, 187, https://doi.org/10.3390/pr7040187.
  7. He, S., Ni, Y., Lu, L., Chai, Q., Yu, T., Shen, Z. & Yang, C. (2020). Simultaneous degradation of n-hexane and production of biosurfactants by Pseudomonas sp. strain NEE2 isolated from oil-contaminated soils, Chemosphere 242, 125237, https://doi.org/10.1016/j.chemosphere.2019.125237.
  8. Martinez-Rojano, H., Mancilla-Ramirez, J., Quiñonez-Diaz, L. & Galindo-Sevilla, N. (2008). Activity of hydroxyurea against Leishmania mexicana, Antimicrobial Agents Chemotheraphy 52, pp. 3642–3647, https://doi.org/10.1128/aac.00124-08.
  9. Miller, U., Sówka, I. & Adamiak, W. (2019). The effect of betaine on the removal of toluene by biofiltration, SN Applied Sciences 1, https://doi.org/10.1007/s42452-019-0832-6.
  10. Miller, U., Sówka, I. & Adamiak, W. (2020). The use of surfactant from the Tween group in toluene biofi ltration, Archives of Environmental Protection, Vol. 46 no. 2 pp. 53–57, DOI: 10.24425/aep.2020.133474.
  11. Mudliar, S., Giri, B., Padoley, K., Satpute, D., Dixit, R., Bhatt, P., Pandey, R., Juwarkar, A. & Vaidya, A. (2010). Bioreactors for treatment of VOCs and odours – A review, Journal of Environmental Management 91, pp. 1039–1054,https://doi.org/10.1016/j.jenvman.2010.01.006.
  12. Purswani, J., Juárez, B., Rodelas, B., Gónzalez-López, J. & Pozo, C. (2011). Biofilm formation and microbial activity in a biofilter system in the presence of MTBE, ETBE and TAME, Chemosphere 85, pp. 616–624, https://doi.org/10.1016/j.chemosphere.2011.06.106.
  13. Ramani, R., Ramani, A. & Wong, S.J. (1997). Rapid Flow Cytometric Susceptibility Testing of Candida albicans, Journal of Clinical Microbiology 35(9):2320-4, DOI: 10.1128/jcm.35.9.2320-2324.1997.
  14. Rybarczyk, P., Szulczyński, B. & Gębicki, J. (2020). Simultaneous Removal of Hexane and Ethanol from Air in a Biotrickling Filter – Process Performance and Monitoring Using Electronic Nose, Sustainability 12, 387, https://doi.org/10.3390/su12010387.
  15. Rybarczyk, P., Szulczyński, B., Gębicki, J. & Hupka, J. (2019a). Treatment of malodorous air in biotrickling filters: A review, Biochemical Engineering Journal 141, pp. 146–162, https://doi.org/10.1016/j.bej.2018.10.014.
  16. Rybarczyk, P., Szulczyński, B., Gospodarek, M. & Gębicki, J. (2019b). Effects of n-butanol presence, inlet loading, empty bed residence time and starvation periods on the performance of a biotrickling filter removing cyclohexane vapors from air, Chemical Papers 74, pp. 1039–1047,https://doi.org/10.1007/s11696-019-00943-2.
  17. Salamanca, D., Dobslaw, D. & Engesser, K.-H. (2017). Removal of cyclohexane gaseous emissions using a biotrickling filter system, Chemosphere 176, pp. 97–107, https://doi.org/10.1016/j.chemosphere.2017.02.078.
  18. Spigno, G., Pagella, C., Fumi, M.D., Molteni, R. & De Faveri, D.M. (2003). VOCs removal from waste gases: Gas-phase bioreactor for the abatement of hexane by Aspergillus niger, Chemical Engineering Science 58, pp. 739–746, https://doi.org/10.1016/S0009-2509(02)00603-6.
  19. Yalkowsky, S.H., He, Y. & Jain, P. (2016). Handbook of Aqueous Solubility Data, Handbook of Aqueous Solubility Data. CRC Press,https://doi.org/10.1201/ebk1439802458.
  20. Yang, C., Chen, H., Zeng, G., Yu, G. & Luo, S. (2010). Biomass accumulation and control strategies in gas biofiltration, Biotechnology Advances 28, 4, pp. 531–540, https://doi.org/10.1016/j.biotechadv.2010.04.002.
  21. Yang, C., Qian, H., Li, X., Cheng, Y., He, H., Zeng, G. & Xi, J. (2018). Simultaneous Removal of Multicomponent VOCs in Biofilters, Trends in Biotechnology 36, 7, pp. 673–685, https://doi.org/10.1016/j.tibtech.2018.02.004.
  22. Zhang, Y., Liss, S.N. & Allen, D.G. (2006). The effects of methanol on the biofiltration of dimethyl sulfide in inorganic biofilters, Biotechnology and Bioengineering 95, pp. 734–743, https://doi.org/10.1002/bit.21033.
  23. Zhang, Y., Liu, J., Qin, Y., Yang, Z., Cao, J., Xing, Y. & Li, J. (2019). Performance and microbial community evolution of toluene degradation using a fungi-based bio-trickling filter, Journal of Hazardous Materials 365, pp. 642–649, https://doi.org/10.1016/j.jhazmat.2018.11.062.
  24. Zhanga, Y., Denga, W., Qina, Y., Yanga, Z., Liua, J. & Lia, J. (2018) Research on Simultaneous Removal of Cyclohexane and Methyl Acetate in Biotrickling Filters, Proceedings of the 2nd International Conference of Recent Trends in Environmental Science and Engineering, Niagara Falls, Canada, https://doi.org/10.11159/rtese18.107.
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Authors and Affiliations

Piotr Rybarczyk
1
ORCID: ORCID
Milena Marycz
1
Bartosz Szulczyński
1
ORCID: ORCID
Anna Brillowska-Dąbrowska
2
Agnieszka Rybarczyk
3
Jacek Gębicki
1
ORCID: ORCID

  1. Department of Process Engineering and Chemical Technology, Faculty of Chemistry, Gdańsk University of Technology
  2. Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology
  3. Department of Histology, Faculty of Medicine, Medical University of Gdańsk
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Abstract

B a c k g r o u n d: The aim of this study was to determine the effect of sesquiterpene lactone parthenolide on the cytotoxic and pro-oxidative effects of etoposide in HL-60 cells.
M e t h o d s: Cytotoxic effects were determined by incubation of HL-60 cells with various concentrations of examined compounds and combinations thereof, which were then stained with propidium iodide and analyzed using a flow cytometer. To determine the role of oxidative stress in the action of the compounds, co-incubation with N-acetyl-l-cysteine (NAC) and parthenolide and/or etoposide was used and the level of reduced glutathione (GSH) was detected.
R e s u l t s: Parthenolide significantly enhanced the cytotoxic and pro-apoptotic effects of etoposide. However, in most cases of the combinations of parthenolide and etoposide, their effect was antagonistic, as confirmed by an analysis using the CalcuSyn program. The examined compounds significantly reduced the level of GSH in HL-60 cells. Combination of etoposide at a concentration of 1.2 μM and parthenolide also significantly reduced GSH level. However, in the case of a combination of etoposide at a concentration of 2.5 μM with parthenolide, a significant increase in the level of GSH was obtained compared to compounds acting alone. This last observation seems to confirm the antagonism between the compounds tested.
C o n c l u s i o n s: Parthenolide did not limit the cytotoxic effect of etoposide in HL-60 cells even in the case of antagonistic interaction. If parthenolide does increase GSH levels in combination with etoposide in the normal hematopoietic cells, it could protect them against the pro-oxidative effects of this anti-cancer drug.
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Bibliography

1. Bell J.A., Galaznik A., Huelin R., Stokes M., Guo Y., Fram R.J., Faller D.V.: Effectiveness and safety of therapeutic regimens for elderly patients with acute myeloid leukemia: a systematic literature review. Clin Lymphoma Myeloma Leuk. 2018; 18: e303–e314.
2. Hackl H., Astanina K., Wieser R.: Molecular and genetic alterations associated with therapy resistance and relapse of acute myeloid leukemia. J Hematol Oncol. 2017; 20: 51.
3. Foran J.M.: Do cytogenetics affect the post-remission strategy for older patients with AML in CR1? Best Pract Res Clin Haematol. 2017; 30: 306–311.
4. Yunos N.M., Beale P., Yu J.Q., Huq F.: Synergism from the combination of oxaliplatin with selected phytochemicals in human ovarian cancer cel lines. Anticancer Res. 2011; 31: 4283–4290.
5. Shah K., Mirza S., Desai U., Jain N., Rawal R.: Synergism of curcumin and cytarabine in the down regulation of multi-drug resistance genes in acute myeloid leukemia. Anticancer Agents Med Chem. 2016; 16: 128–135.
6. Banudevi S., Swaminathan S., Maheswari K.U.: Pleiotropic role of dietary phytochemicals in cancer: emerging perspectives for combinational therapy. Nutr Cancer. 2015; 67: 1021–1048.
7. Pei S., Minhajuddin M., D’Alessandro A., Nemkov T., Stevens B.M., Adane B., Khan N., Hagen F.K., Yadav V.K., De S., Ashton J.M., Hansen K.C., Gutman J.A., Pollyea D.A., Crooks P.A., Smith C., Jordan C.T.: Rational design of a parthenolide-based drug regimen that selectively eradicates acute myelogenous leukemia stem cells. J Biol Chem. 2016; 291: 21984–22000.
8. Guzman M.L., Rossi R.M., Karnischky L., Li X., Peterson D.R., Howard D.S., Jordan C.T.: The sesquiterpene lactone parthenolide induces apoptosis of human acute myelogenous leukemia stem and progenitor cells. Blood. 2005; 105: 4163–4169.
9. Papiez M.A., Baran J., Bukowska-Straková K., Wiczkowski W.: Antileukemic action of (-)-epicatechin in the spleen of rats with acute myeloid leukemia. Food Chem Toxicol. 2010; 48: 3391–3397.
10. Papież M.A.: The influence of curcumin and (-)-epicatechin on the genotoxicity and myelosuppression induced by etoposide in bone marrow cells of male rats. Drug Chem Toxicol. 2013; 36: 93–101.
11. Siveen K.S., Uddin S., Mohammad R.M.: Targeting acute myeloid leukemia stem cel signaling by natural products. Mol Cancer. 2017; 16: 1–12.
12. Curry E.A., Murry D.J., Yoder C., Fife K., Armstrong V., Nakshatri H., O’Connell M., Sweeney C.J.: Phase I dose escalation trial of feverfew with standardized doses of parthenolide in patients with cancer. Invest New Drugs. 2004; 22: 299–305.
13. Knight D.W.: Feverfew: chemistry and biological activity. Nat Prod Rep. 1995; 12: 271–276.
14. Ordóñez P.E., Sharma K.K., Bystrom L.M., Alas M.A., Enriquez R.G., Malagón O., Jones D.E., Guzman M.L., Compadre C.M.: Dehydroleucodine, a Sesquiterpene Lactone from Gynoxys verrucosa, Demonstrates Cytotoxic Activity against Human Leukemia Cells. J Nat Prod. 2016; 79: 691–696.
15. Merfort I.: Perspectives on sesquiterpene lactones in inflammation and cancer. Curr Drug Targets. 2011; 12: 1560–1573.
16. Li C., Jones A.X., Lei X.: Natural product reports synthesis and mode of action of oligomeric sesquiterpene lactones. Nat Prod Rep. 2015; 1–10.
17. Pei S., Minhajuddin M., Callahan K.P., Balys M., Ashton J.M., Neering S.J., Lagadinou E.D., Corbett C., Ye H., Liesveld J.L., O’Dwyer K.M., Li Z., Shi L., Greninger P., Settleman J., Benes C., Hagen F.K., Munger J., Crooks P.A., Becker M.W., Jordan C.T.: Targeting aberrant glutathione metabolism to eradicate human acute myelogenous leukemia cells. J Biol Chem. 2013; 288: 33542–33558.
18. Klein K., Kaspers G., Harrison C.J., Beverloo H.B., Reedijk A., Bongers M., Cloos J., Pession A., Reinhardt D., Zimmerman M., Creutzig U., Dworzak M., Alonzo T., Johnston D., Hirsch B., Zapotocky M., De Moerloose B., Fynn A., Lee V., Taga T., Tawa A., Auvrignon A., Zeller B., Forestier E., Salgado C., Balwierz W., Popa A., Rubnitz J., Raimondi S., Gibson B.: Clinical impact of additional cytogenetic aberrations, ckit and ras mutations, and treatment elements in pediatric t(8;21)-aml: results from an international retrospective study by the international Berlin–Frankfurt–Münster study group. J Clin Oncol. 2015; 20: 4247–4258.
19. Burnett A.K.: New induction and postinduction strategies in acute myeloid leukemia. Curr Opin Hematol. 2012; 19: 76–81.
20. Kagan V.E., Yalowich J.C., Borisenko G.G., Tyurina Y.Y., Tyurin V.A., Thampatty P., Fabisiak J.P.: Mechanism-based chemopreventive strategies against etoposide-induced acute myeloid leukemia: free radical/antioxidant approach. Mol Pharmacol. 1999; 56: 494–506.
21. Patel N.M., Nozaki S., Shortle N.H., Bhat-Nakshatri P., Newton T.R., Rice S., Gelfanov V., Boswell S.H., Goulet R.J., Sledge G.W., Nakshatri H.: Paclitaxel sensitivity of breast cancer cells with constitutively active NF-kappaB is enhanced by Ikappa-B alpha super-repressor and parthenolide. Oncogene. 2000; 19: 4159–4169.
22. deGraffenried L.A., Chandrasekar B., Friedrichs W.E., Donzis E., Silva J., Hidalgo M., Freeman J.W., Weiss G.R.: NF-kappa B inhibition markedly enhances sensitivity of resistant breast cancer tumor cells to tamoxifen. Ann Oncol. 2004; 15: 885–890.
23. Tietze F.: Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Ann Biochem. 1969; 27: 502–522.
24. Papież M.A., Krzyściak W., Szade K., Bukowska-Straková K., Kozakowska M., Hajduk K., Bystrowska B., Dulak J., Jozkowicz A.: Curcumin enhances the cytogenotoxic effect of etoposide in leukemia cells through induction of reactive oxygen species. Drug Des Devel Ther. 2016; 10: 557–570.
25. Wurthwein G., Krumpelmann S., Tillmann B., Real E., Schulze-Westhoff P., Jurgens H., Boos J.: Population pharmacokinetic approach to compare oral and i.v. administration of etoposide. Anticancer Drugs. 1999; 10: 807–814.
26. Kim Y.R., Eom J.I., Kim S.J., Jeung H.K., Cheong J.W., Kim J.S., Min Y.H.: Myeloperoxidase expression as a potential determinant of parthenolide-induced apoptosis in leukemia bulk and leukemia stem cells. JPET. 2010; 335: 389–400.
27. Vlasova I.I., Feng W., Goff J.P., Giorgianni A., Do D., Gollin S.M., Lewis D.W., Kagan V.E., Yalowich J.C.: Myeloperoxidase-dependent oxidation of etoposide in human myeloid progenitor CD34+ cells. Mol Pharmacol. 2011; 79: 448–479.
28. Seo K.H., Ko H.M., Han A., Kim H.A., Choi J.H., Park S.J., Kim K.J., Lee H.K., Im S.Y.: Platelet-activating factor induces up-regulation of antiapoptotic factors in a melanoma cell line through nuclear factor-kb activation. Cancer Res. 2006; 66: 4681–4686.
29. Teufelhofer O., Weiss R.M., Parzefall W., Schulte-Hermann R., Micksche M., Berger W., Elbling L.: Promyelocytic HL60 cells express NADPH oxidase and are exellent targets in a rapid spectrophotometric microplate assay for extracellular superoxide. Toxicol Sci. 2003; 76: 376–383.
30. Skalska J., Brookes P.S., Nadtochiy S.M., Hilchey S.P., Jordan C.T., Guzman M.L., Maggirwar S.B., Briehl M.M., Bernstein S.H.: Modulation of cell surface protein free thiols: a potential novel mechanism of action of the sesquiterpene lactone parthenolide. PLoS One. 2009; 2: e8115.
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Authors and Affiliations

Monika A. Papież
1
Oliwia Siodłak
1
Wirginia Krzyściak
2

  1. Department of Cytobiology, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland
  2. Department of Medical Diagnostic, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland
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Abstract

We used germination tests to assess the frequency of polyembryony in 9 asparagus cultivars with a high propensity to produce double embryos with different ploidy levels: Alpha, Andreas, Boonlim, Cipres, Eposs, Helios, Limbras, Ravel and Sartaguda. Twin embryos inside a single seed were found in 3 cultivars: Eposs 2n, Ravel 2n and Sartaguda 2n, at 0.60% frequency (15 seeds with twin embryos out of 2500 seeds). Of 30 obtained seedlings, 14 were separated diploid-diploid twins, 6 were conjoined diploid pairs, 8 were separated diploid-haploid and 2 were diploid-haploid pairs conjoined in the hypocotyl region. Some embryos showed unilateral dominance of one embryo (size and shape). The haploid status of the smallest embryo was confirmed by chromosome number (n=x=10) and flow cytometry (nuclear C DNA amount 1.95 pg). The haploid obtained in this manner possessed enough vegetative vigor to undergo chromosome doubling.

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Authors and Affiliations

Maciej Zenkteler
Weronika Dębowska
Mikołaj Knaflewski
Elżbieta Zenkteler

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