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Number of results: 106
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Abstract

This paper present the results of experimental investigations of condensation of R134a refrigerant in pipe minichannels with internal diameters 0.64, 0.90, 1.40, 1.44, 1.92 and 3.30 mm subject to periodic pressure instabilities. It was established that as in conventional channels, the displacement velocity of the pressure instabilities distinctly depends on the frequency of their hydrodynamic generation. The void fraction distinctly influences the velocity of the pressure instabilities. The form of this relationship depends on the internal diameter of the minichannels and on the method of calculating the void fraction.

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Authors and Affiliations

Waldemar Kuczyński
Henryk Charun
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Abstract

The article presents the results of plasma vitrification of solid remnants from thermal waste treatment with and without the addition of a carbonate fraction obtained from lead-zinc ore flotation. The substrates used in the research were slags and ashes from medical waste treatment, incineration of municipal waste, sewage sludge as well as hazardous and industrial wastes.

The plasma treatment resulted in acquiring products of different quality depending on the processed substrate. Most of the obtained products were of vitreous and homogenic build. Treatment of remnants from incineration of hazardous and industrial wastes resulted in obtaining heterogeneous and rough surfaced products. A 20% share of the carbonate fraction enabled the obtaining of a vitrified product with a glassy surface and fracture. Hardness of the obtained products was rated in Mohs scale and ranged from 6 to 6,5. Leaching tests showed a decrease in heavy metal concentration in the leachates from vitrificates with the addition of carbonate fraction compared to the ones with it.

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Authors and Affiliations

January Bień
Bartosz Morzyk
Katarzyna Wystalska
Piotr Celary
Jolanta Sobik-Szołtysek
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Abstract

Technology advancements entail a necessity to remove huge amounts of heat produced by today’s electronic devices based on highly integrated circuits, major generators of heat. Heat transfer to boiling liquid flowing through narrow minichannels is a modern solution to the problem of heat transfer enhancement. The study was conducted for FC-72 boiling in a rectangular, vertical and asymmetrically heated minichannel that had depths of 0.5-1.5 mm, a width of 20 mm and a length of 360 mm. The heat flux increased and decreased within the range of 58.3-132.0 kWm−2, the absolute pressure ranged from 0.116 to 0.184 MPa and the mass flux was 185-1139.2 kgm−2s−1. The boiling process took place on a flat vertical heating surface made of Haynes-230 0.1 mm thick acid-proof rolled plate with the surface roughness of 121 μm.

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Authors and Affiliations

Robert Kaniowski
Mieczysław Poniewski
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Abstract

Contamination of soil with heavy metals has become a worldwide environmental problem, and receives great attention. In this study, we aim to investigate soil pollution level affected by an industrial district nearby. The total amount of typical heavy metals in the soils (Hengyang Songmu Industrial Park, Hunan Province, China) was analyzed. In addition, the fraction analysis and laboratory simulation leaching via different pH rainwater was carried out to study the migration and transformation of heavy metals. The main results show that the contents of Cu, Zn, Pb, Cr and Cd in the samples were higher than the soil background values in Hunan Province. The heavy metals forms, analyzed by sequential extraction method, show that the proportion of the unstable form of Cd, Zn and Pb was more than 50%. Igeo values indicate that the heavy metal pollution degree of soil sample #5 at the investigated area is recorded in the order of Cd(6.42), Zn(2.28), Cu(1.82), Pb(1.63), and Cr(0.37). Cu, Zn, Pb, Cr and Cd in this area could pose a potential leaching risk to the environment which may affect the food chain and constitute a threat to human health. It would be necessary to take steps to stabilize and monitor the heavy metals in soil.

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Authors and Affiliations

Wenfa Tan
Yuan Li
Lei Ding
Yachao Wang
Jiangxiang Li
Qinwen Deng
Feng Guo
Xue Xiao
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Abstract

This paper presents the content changes in the Chemical Oxygen Demand (COD) solubilised in hydrolisates obtained from thermally disintegrated municipal waste biofractions. A series of tests related to biowaste undergoing thermal treatment at the following temperatures: 55, 75, 95, 115, 135, 155 and 175°C were conducted for 0.5, 1 and 2 hours. The highest increase in COD solid fraction solubilisation (238%) was observed for the samples disintegrated at 175°C for 2 hours. The values of the reaction rate coefficient k20 = 0.6 d-1 and temperature coefficient θ = 1.023 were determined. Statistical analysis of the multiple regression (correlation coefficient R = 0.89) showed that the temperature has a greater impact on COD solid fraction solubilisation - determined β = 0.66. The multiple correlation coefficient for the treatment time was β = 0.61.

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Authors and Affiliations

Sylwia Myszograj
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Abstract

The paper presents the application of liquid crystal thermography for temperature determination and visualisation of two phase flow images on the studied surface. Properties and applications of thermochromic liquid crystals are discussed. Liquid crystals were applied for two-dimensional detection of the temperature of the heating foil forming one of the surfaces of the minichannel along which the cooling liquid flowed. The heat flux supplied to the heating surface was altered in the investigation and it was accompanied by a change in the color distribution on the surface. The accuracy of temperature measurements on the surface with liquid crystal thermography is estimated. The method of visualisation of two-phase flow structures is described. The analysis of monochrome images of flow structures was employed to calculate the void fraction for some cross-sections. The flow structure photos were processed using Corel graphics software and binarized. The analysis of phase volumes employed Techsystem Globe software. The measurement error of void fraction is estimated.

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Authors and Affiliations

Magdalena Piasecka
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Abstract

The contents of total P and its bioavailable forms. as well as of Fe, Al, Mn, Ca, and OM in the bottom sediments of the Solina-Myczkowce (S-E Poland) cascade of reservoirs, are presented. Notwithstanding a relatively low calcium content, it is the apatite fraction that accounts for the largest share of total phosphorus in the shallower parts of the Solina and Myczkowce Reservoirs. In turn, while contents of iron and aluminium (and manganese in the Solina Reservoir) are high, the fraction containing non-apatite inorganic phosphorus accounts for the smallest portion of the total phosphorus in the bottom sediments of both reservoirs. Bottom sediments of the Solina Reservoir are also characterised by significant correlations between total phosphorus content and aluminium content. Otherwise, significant correlations are reported for only some of the stations at each of the reservoirs.
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Authors and Affiliations

Lilianna Bartoszek
Janusz A. Tomaszek
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Abstract

This paper addresses the nonlinear Cucker–Smale optimal control problem under the interplay of memory effect. The aforementioned effect is included by employing the Caputo fractional derivative in the equation representing the velocity of agents. Sufficient conditions for the existence of solutions to the considered problem are proved and the analysis of some particular problems is illustrated by two numerical examples.

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Authors and Affiliations

Ricardo Almeida
Rafał Kamocki
Agnieszka B. Malinowska
Tatiana Odzijewicz
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Abstract

In this paper, we consider an optimal control problem in which a dynamical system is controlled by a nonlinear Caputo fractional state equation. First we get the linearized maximum principle. Further, the concept of a quasi-singular control is introduced and, on this basis, an analogue of the Legendre-Clebsch conditions is obtained. When the analogue of Legendre- Clebsch condition degenerates, a necessary high-order optimality condition is derived. An illustrative example is considered.
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Authors and Affiliations

Shakir Sh. Yusubov
1
Elimhan N. MahmudoV
2 3
ORCID: ORCID

  1. Department of Mechanics and Mathematics, Baku State University, Baku, Azerbaijan
  2. Department of Mathematics, Istanbul Technical University, Istanbul, Turkey
  3. Azerbaijan National Aviation Academy, Baku, Azerbaijan
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Abstract

The paper presents results of aluminium concentration determination in the samples of surface water and bottom sediments of the Mala Wełna River (West Poland). In the surface water the concentration of aluminium varies in the range from 4.14 to 25.9 ug/dm'. With use of the Mineql+ program the concentration of the aluminium sulphate complexes in the water samples studied has been determined in a model way. In the bottom sediments samples of the river aluminium has been determined in the granulometrie fractions of the grain sizes> 2.0; 2.0-1.0; 1.0-0.5; 0.5-0.25; 0.25-0.1; 0.1-0.063; < 0.063 mm, using the sequential extraction scheme proposed by Tessier el al. The lowest concentration of aluminium has been found in the granulometrie fraction 0.5-0.25 mm, while the highest in the fractions 0.1-0.063 and< 0.063 mm. An elevated concentration of aluminium has been also noted in the fraction> 2.0 mm. Taking into regard the chemical fractions the lowest concentration of aluminium has been found in the exchange fraction and the fraction bounded to carbonates (fractions I and li), whereas the highest concentration of aluminium has been determined in the lithogenic fraction (fraction V). The methods of sample preparation for analysis of aluminium in bottom sediments were compared. Il was observed that higher concentration of aluminium was present in grounded samples without its influence on grain size fractions. The concentration of aluminium in surface water samples has been determined by the GF-AAS, while in bottom sediments by F-AAS.
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Authors and Affiliations

Marcin Frankowski
Anetta Zioła-Frankowska
Jerzy Siepak
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Abstract

The use of fractional-order calculus for system modeling is a good alternative to well-known classic integer-order methods, primarily due to the precision with which the modeled object may be mapped. In this study, we created integer and fractional discrete models of a real object – a highspeed brushless micro-motor. The accuracy of the models was verified and compared.

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Authors and Affiliations

M. Matusiak
M. Bąkała
R. Wojciechowski
P. Ostalczyk
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Abstract

The void fraction is one of the most important parameters characterizing a multiphase flow. The prediction of the performance of any system operating with more than single phase relies on our knowledge and ability to measure the void fraction. In this work, a validated simulation study was performed in order to predict the void fraction independent of the flow pattern in gas-liquid two-phase flows using a gamma ray 60Co source and just one scintillation detector with the help of an artificial neural network (ANN) model of radial basis function (RBF). Three used inputs of ANN include a registered count under Compton continuum and counts under full energy peaks of 1173 and 1333 keV. The output is a void fraction percentage. Applying this methodology, the percentage of void fraction independent of the flow pattern of a gas-liquid two-phase flow was estimated with a mean relative error less than 1.17%. Although the error obtained in this study is almost close to those obtained in other similar works, only one detector was used, while in the previous studies at least two detectors were employed. Advantages of using fewer detectors are: cost reduction and system simplification.
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Authors and Affiliations

Roshani Gholam H.
Nazemi Ehsan
Shama Farzin
Imani Mohammad A.
Mohammadi Salar
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Abstract

B a c k g r o u n d: A novel paradigm of diastolic heart failure with preserved ejection fraction (HFpEF) proposed the induction of coronary microvascular dysfunction by HFpEF comorbidities via a systemic pro-infl ammatory state and associated oxidative stress. Th e consequent nitric oxide deficiency would increase diastolic tension and favor fi brosis of adjacent myocardium, which implies not only left ventricular (LV), but all-chamber myocardial stiff ening. Our aim was to assess relations between low-grade chronic systemic infl ammation and left atrial (LA) pressure-volume relations in real-world HFpEF patients.

Me t h o d s: We retrospectively analyzed medical records of 60 clinically stable HpEFF patients in sinus rhythm with assayed high-sensitive C-reactive protein (CRP) during the index hospitalization. Subjects with CRP >10 mg/L or coexistent diseases, including coronary artery disease, were excluded. LV and LA diameters and mitral E/E’ ratio (an index of LA pressure) were extracted from routine echocardiographic 46 Cyrus M. Sani, Elahn P.L. Pogue, et al. records. A surrogate measure of LA stiff ness was computed as the averaged mitral E/e’ ratio divided by LA diameter.

R e s u l t s: With ascending CRP tertiles, we observed trends for elevated mitral E/e’ ratio (p <0.001), increased relative LV wall thickness (p = 0.01) and higher NYHA functional class (p = 0.02). Th e LA stiffness estimate and log-transformed CRP levels (log-CRP) were interrelated (r = 0.38, p = 0.003). On multivariate analysis, the LA stiff ness index was independently associated with log-CRP (β ± SEM: 0.21 ± 0.07, p = 0.007) and age (β ± SEM: 0.16 ± 0.07, p = 0.03), which was maintained upon adjustment for LV mass index and relative LV wall thickness.

C o n c l u s i o n s: Low-grade chronic infl ammation may contribute to LA stiff ening additively to age and regardless of the magnitude of associated LV hypertrophy and concentricity. LA stiff ening can exacerbate symptoms of congestion in HFpEF jointly with LV remodeling.

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Authors and Affiliations

Cyrus M. Sani
Elahn P.L. Pogue
Joanna B. Hrabia
Alexander G. Zayachkowski
Magdaline M. Zawadka
Adrian G. Poniatowski
Dorota Długosz
Wiktoria Leśniak
Olga Kruszelnicka
Bernadeta Chyrchel
Andrzej Surdacki
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Abstract

In the paper a new, state space, fully discrete, fractional model of a heat transfer process in one dimensional body is addressed. The proposed model derives directly from fractional heat transfer equation. It employes the discrete Grünwald-Letnikov operator to express the fractional order differences along both coordinates: time and space. The practical stability and numerical complexity of the model are analysed. Theoretical results are verified using experimental data.
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Bibliography

  1.  S. Das, Functional Fractional Calculus for System Identification and Controls, Springer, Berlin, 2010.
  2.  R. Caponetto, G. Dongola, L. Fortuna, and I. Petras, “Fractional order systems: Modeling and Control Applications”, in World Scientific Series on Nonlinear Science, ed. L.O. Chua, pp. 1–178, University of California, Berkeley, 2010.
  3.  A. Dzieliński, D. Sierociuk, and G. Sarwas, “Some applications of fractional order calculus”, Bull. Pol. Ac.: Tech. 58(4), 583– 592 (2010).
  4.  C.G. Gal and M. Warma, “Elliptic and parabolic equations with fractional diffusion and dynamic boundary conditions”, Evol. Equ. Control Theory 5(1), 61–103 (2016).
  5.  E. Popescu, “On the fractional Cauchy problem associated with a feller semigroup”, Math. Rep. 12(2), 81–188 (2010).
  6.  D. Sierociuk et al., “Diffusion process modeling by using fractional-order models”, Appl. Math. Comput. 257(1), 2–11 (2015).
  7.  J.F. Gómez, L. Torres, and R.F. Escobar (eds.), “Fractional derivatives with Mittag-Leffler kernel trends and applications in science and engineering”, in Studies in Systems, Decision and Control, vol. 194, ed. J. Kacprzyk, pp. 1–339. Springer, Switzerland, 2019.
  8.  M. Dlugosz and P. Skruch, “The application of fractional-order models for thermal process modelling inside buildings”, J. Build Phys. 1(1), 1–13 (2015).
  9.  A. Obrączka, Control of heat processes with the use of noninteger models. PhD thesis, AGH University, Krakow, Poland, 2014.
  10.  A. Rauh, L. Senkel, H. Aschemann, V.V. Saurin, and G.V. Kostin, “An integrodifferential approach to modeling, control, state estimation and optimization for heat transfer systems”, Int. J. Appl. Math. Comput. Sci. 26(1), 15–30 (2016).
  11.  T. Kaczorek, “Singular fractional linear systems and electri cal circuits”, Int. J. Appl. Math. Comput. Sci. 21(2), 379–384 (2011).
  12.  T. Kaczorek and K. Rogowski, Fractional Linear Systems and Electrical Circuits, Bialystok University of Technology, Bialystok, 2014.
  13.  I. Podlubny, Fractional Differential Equations, Academic Press, San Diego, 1999.
  14.  B. Bandyopadhyay and S. Kamal, “Solution, stability and realization of fractional order differential equation”, in Stabilization and Control of Fractional Order Systems: A Sliding Mode Approach, Lecture Notes in Electrical Engineering 317, pp. 55–90, Springer, Switzerland, 2015.
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  16.  P. Ostalczyk, “Equivalent descriptions of a discrete-time fractional-order linear system and its stability domains”, Int. J. Appl. Math. Comput. Sci. 22(3), 533–538 (2012).
  17.  E.F. Anley and Z. Zheng, “Finite difference approximation method for a space fractional convection–diffusion equation with variable coefficients”, Symmetry 12(485), 1–19 (2020).
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  21.  D. Mozyrska and E. Pawluszewicz, “Fractional discrete-time linear control systems with initialization”, Int. J. Control 1(1), 1–7 (2011).
  22.  K. Oprzędkiewicz, “The interval parabolic system”, Arch. Control Sci. 13(4), 415–430 (2003).
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Authors and Affiliations

Krzysztof Oprzędkiewicz
1
ORCID: ORCID

  1. AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059 Kraków, Poland
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Abstract

The efficient, stable and reliable operation of the blast furnace secures the proper quality of coke, which is one of the basic components of the blast furnace charge. In modern blast-furnace technology, when using substitute fuels, i.e. coal dust, the role of coke is extremely important. For this reason, the demands placed on its quality increase. Domestic coking plants have a limited base of Polish high quality coking coals at their disposal, therefore the full use of their coking properties is extremely important. The grain composition of the coal blend is one of the basic factors affecting the quality of the produced coke. This influence depends on the quantity and quality of coal components that make up the blend. In the conducted research, 21 coking coals, differing significantly in the degree of rank and origin (Polish and overseas coals), it was shown that the separated grain classes differ in properties, both coking properties and the degree of devolatalization during heating. In analyzing the obtained results, it was observed that the grain volume growth occurs essentially in the temperature range between the beginning and the maximum of fluidity. It has been shown that there is a linear correlation between the temperature corresponding to maximum fluidity and the temperature at which the maximum rate of evolution of volatiles enters. The presented phenomena accompany the emergence of coal expansion pressure during the coking process and they are its primary causes. The presented results can be an important guide for preparing the milling of coal for the coking process.

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Authors and Affiliations

Bartosz Mertas
Marek Ściążko
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Abstract

This study investigates Thomas’ cyclically symmetric attractor dynamics with mathematical and electronic simulations using a proportional fractional derivative to comprehend the dynamics of a given chaotic system. The three-dimensional chaotic flow was examined in detail with Riemann-Liouville derivative for different values of the fractional index to highlight the sensitivity of chaotic systems with initial conditions. Thus, the dynamics of the fractional index system were investigated with Eigenvalues, Kaplan–Yorke dimension, Lyapunov exponent, and NIST testing, and their corresponding trajectories were visualized with phase portraits, 2D density plot, and Poincaré maps. After obtaining the results, we found that the integer index dynamics are more complex than the fractional index dynamics. Furthermore, the chaotic system circuit is simulated with operational amplifiers for different fractional indices to generate analog signals of the symmetric attractor, making it an important aspect of engineering. The qualitative application of our nonlinear chaotic system is then applied to encrypt different data types such as voice, image, and video, to ensure that the developed nonlinear chaotic system can widely applied in the field of cyber security.
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Authors and Affiliations

NajeebAlam Khan
1
Muhammad Ali Qureshi
2
Saeed Akbar
1
Asmat Ara
3

  1. Department of Mathematics, University of Karachi, Karachi 75270, Pakistan
  2. Department of Physics, University of Karachi, Karachi 75270, Pakistan
  3. College of Humanities and Sciences, PAF-KIET, Karachi 75190, Pakistan
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Abstract

Generation of coal-based electricity is always associated with the origination of large amount of combustion waste. The presented article is a review concerning the possibilities of innovative directions of management for one of the by-products of coal combustion: fly ash. The storage of these waste products is associated with their negative impact on the environment. This is why research has been undertaken worldwide on the implementation of the concept of a circular economy. This article includes the examination of basic physical, chemical, and mineralogical properties of the most valuable components of fly ash (microspheres, magnetic fraction, and glass). It contains the examination of methods of separating these components and indicates the prospective directions of their use, e.g. as light fillers for polymers, sorbents, catalysts, composite materials, light ceramics, lightweight concretes, thermal insulation materials, biomaterials, raw material for the synthesis of zeolites or geopolymers. The paper also presents the components of fly ash, which can be treated as an alternative source of valuable elements, including critical elements. Moreover, it points to the necessity of capturing flammable substances from combustion by-products in order to obtain raw material characterised by a high degree of purity. It has been demonstrated that this way of ash management can lead to high recycling rates and bring valuable materials back to the economy. Such actions fit perfectly into global efforts for sustainable development and the circular economy.
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Authors and Affiliations

Ewa Strzałkowska
1
ORCID: ORCID

  1. Silesian University of Technology, Gliwice, Poland
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Abstract

The aim of the study was fractionation of Zn, Cu, Ni, Pb in the bottom sediments of two small reservoirs: at Krempna on the Wisłoka River and at Zcslwicc on the Dlubnia River. The partitioning of metals for various fractions was performed with the use of Tessier's sequential chemical extraction method. All together five metal fractions were distinguished: exchangeable metals (fraction I), metals bound to carbonates (fraction II), metals bound to hydrate Fe-Mn oxides (fraction III), metals bound to organic matter (fraction IV), and metals bound to minerals (fraction V). The largest quantities of metals were bound with fraction V, the smallest occurred in the forms most easily available for living organisms, in faction I. Proportions ofZn, Ni and Pb in the exchangeable fraction were about 1%. The amounts of metals bound with fraction II were also relatively low, except lor Zn in bottom sediment at the Zeslawice Reservoir. In this bottom sediment the share of Zn bound to carbonates was 33%. Medium metal quantities were associated with hydrate Fe-Mn oxides (fraction III) and with organic matter (fraction IV). Relatively high proportion ofmetals in fraction V and trace amounts ofmetals in fraction I as well as alkaline and neutral reaction of the sediments may prove a potentially low hazard of the metal release in the case of chemical changes in the reservoirs.
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Authors and Affiliations

Marek Madeyski
Marek Tarnawski
Czesława Jasiewicz
Agnieszka Baran
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Abstract

We compared different net sampling methods for microplastic quantitative collection by sampling different water volumes with nets of different mesh sizes. Sampling covered freshwater lake and reservoir with a significant degree of eutrophication located in Central Poland. The fibres were the main type of plastic collected from sampling sites and constituted 83% of all microplastic particles. Fibres of 700–1900 μm dominated in the samples. The size of mesh affected the amount of fibres collected. Small fibres of 10–200 μm in length were collected using only a fine net of 20 μm mesh size. The total amount of fibres depended on sample volumes; concentrations of microplastics were higher for smaller water volumes. It is likely that clogging with phytoplankton and suspended particles reduced the filtration capacity of the finest nets when large volumes were sampled, which led to an underestimation of microplastic. To our knowledge, this is the first study to provide evidence that the amount of small microfibres depends on mesh size and that the total microplastic abundance in freshwaters in Poland depends on the sample volume. We suggest sampling rather larger than smaller water volumes to assess the level of microplastic contamination more accurately, but clogging, which reduces the filtration capacity of finest nets, should be taken into account when eutrophic freshwater environments are studied.
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Authors and Affiliations

Kamil Karaban
1
ORCID: ORCID
Agnieszka Poniatowska
1
ORCID: ORCID
Anita Kaliszewicz
1
ORCID: ORCID
Michał Winczek
1
ORCID: ORCID
Krassimira Ilieva-Makulec
1
ORCID: ORCID
Jerzy Romanowski
1
ORCID: ORCID

  1. Cardinal Stefan Wyszyński University in Warsaw, Institute of Biological Sciences, Warsaw, Poland
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Abstract

In our recent study we demonstrated that the holding of fresh semen in fractionated seminal plasma (SP1, >40 kDa; SP2, <40 kDa), obtained by gel filtration chromatography, significantly improved the sperm quality characteristics following cryopreservation (Wasilewska-Sakowska et al. 2019). In this study we investigated the effect of post-thaw (PT) supplementation of fractionated SP (SP1 and SP2) on the survival of spermatozoa from boars with good and poor semen freezability, GSF and PSF, respectively. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis showed distinct differences in the protein profiles of SP1 and SP2 from boars with GSF or PSF regarding the number of protein spots. Sperm motility characteristics and the motion patterns, assessed using the computer-assisted sperm analysis (CASA) system, were markedly higher in PT semen supplemented with SP1 and SP2 from boars with GSF. Post-thaw supplementation of either SP1 or SP2 from boars with GSF significantly improved mitochondrial function, plasma membrane and acrosome integrity, and viability during storage. The findings of this study have confirmed that the presence of protective protein components in varying abundance in either fractionated SP from boars with good freezability ejaculates significantly improved the sperm survival following PT storage.

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Authors and Affiliations

K. Wasilewska-Sakowska
Ł. Zasiadczyk
L. Fraser
J. Strzeżek
K. Karpiesiuk
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Abstract

The global stability of positive continuous-time standard and fractional order nonlinear feedback systems is investigated. New sufficient conditions for the global stability of these classes of positive nonlinear systems are established. The effectiveness of these new stability conditions is demonstrated on simple examples of positive nonlinear systems.

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Authors and Affiliations

T. Kaczorek
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Abstract

The aim of this study was to investigate the effect of different extenders on the post-thaw (PT) quality of sperm originating from the sperm-rich fraction (SRF) and post-sperm-rich fraction (PSRF) of boar ejaculate. Motility and velocity parameters, analyzed using a computer-assisted semen analysis (CASA) system, and membrane integrity parameters were markedly higher in frozen-thawed (FT) spermatozoa of the SRF in both the Belstville Thawing Solution (BTS) and Androhep Plus (AHP) extenders, irrespective of the post-thaw (PT) storage time. Furthermore, reduced cryo-survival was more marked in FT spermatozoa of the PSRF in both extenders following storage for 60 min. It was found that the SRF-stored samples in the AHP extender for 60 min exhibited significantly higher percentages of spermatozoa with total motility, mitochondrial function and acrosome integrity than those stored in the BTS extender. The findings of this study confirm that components of the ejaculate fractions and extender have varying effects on the cryo-survival of boar spermatozoa.
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Bibliography

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7. Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
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Authors and Affiliations

Ł. Zasiadczyk
1
K. Kurpanik
1
L. Fraser
1
W. Kordan
1

  1. Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland
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Abstract

The use of foam fractionation followed by aqueous two-phase extraction has emerged as a potential alternative to traditional liquid chromatography, hitherto irreplaceable in the purification of phycobiliproteins. The crude extracts of C-phycocyanin and allophycocyanin were obtained after Thermosynechococcus PCC 6715 biomass disintegration. The FF process with air flow of 2.4 L·h -1 resulted in purification factors up to 1.47 and partitioning coefficients of about 39, and did not require the addition of surfactants. A temperature of 35˚C allowed for the highest partitioning coefficient of 67.6 and yield of 76%; however, the purity of C-PC in condensate at this temperature was lower than at 25˚C. ATPE was tested in 20 different systems consisting of polyethylene glycol and phosphate or citrate salts, of which PEG1500-citrate gave the highest purification factor value of 2.31. Conversely, a partitioning coefficient of 2416 and 1094 were obtained for the PEG1500-phosphate and PEG3000-phosphate systems, respectively. Interestingly, the use of FF condensate in subsequent ATPE step resulted, for the first time, in the separation of the polymer phase into two fractions, one contained C-phycocyanin and the other allophycocyanin. It can be concluded that the use of a two-step system of FF and ATPE is a viable way to separate phycobiliproteins.
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Authors and Affiliations

Anna Antecka
1
ORCID: ORCID
Rafał Szeląg
1
Stanisław Ledakowicz
1
ORCID: ORCID

  1. Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wolczanska 213, 93-005 Lodz, Poland
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Abstract

The global stability of discrete-time nonlinear systems with descriptor positive linear parts and positive scalar feedbacks is addressed. Sufficient conditions for the global stability of standard and fractional nonlinear systems are established. The effectiveness of these conditions is illustrated on numerical examples.

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Authors and Affiliations

Tadeusz Kaczorek
ORCID: ORCID
Andrzej Ruszewski
ORCID: ORCID

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