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Abstract

The shock is a general, non-specific pathological process, caused by the sudden action of very brutal pathogens, a situation for which the body has no reserves for qualitative and quantitative compensation-adaptation. The objective of our experiment was to make an evaluation of the changes in some hematological and biochemical parameters of the blood, during some hypovolemic evolutions, in the rabbits. Twenty New Zealand White rabbits we used. An IDEXX ProCyte Dx Hematology Analyzer was applied to perform hematological determinations. An IDEXX VetTest Chemistry Analyzer was used to perform blood biochemistry determinations. The data obtained were statistically analyzed, calculating the Media and Standard Deviation (SD), using the Microsoft Excel application. At the same time, the statistical significance of the differences between the batches was calculated based on the t test (Student) using the Microsoft Excel application. The study revealed a decrease in the number of red blood cells and leukocytes per unit volume of blood (p<0.05) in the case of group 2 and an increase in glucose, triglycerides (p<0.05).
Experimental hypovolemia induced in the conditions of our experiment determined: an obvious posthemorrhagic anemia, a significant leukopenia mainly 6 hours after the production of hypovolemic shock and a significant hyperglycemia, manifested mainly 12 hours after the induction of hypovolemia.
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Bibliography


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Authors and Affiliations

G. Cotor
1
G. Zagrai
2
G. Gâjâilă
1
M. Ghiță
1
A.M. Ionescu
1
A. Damian
2
A.M. Zagrai (Măierean)
2
Ș. Dragosloveanu
3
D.C. Cotor
2 3

  1. Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Bucharest-050097, Splaiul Independentei 105, Bucharest, Romania
  2. Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca-400372, Calea Manastur 3-5, Cluj-Napoca, Romania
  3. Clinical Hospital of Orthopedics, Traumatology and Osetoarticular TB “Foișor”, Bucharest-030167, Bd. Ferdinand nr. 35-37, Romania
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Abstract

From the regulatory point of view a strong link between an animal model and human pharmacodynamics of biological drugs is very important to qualify the model as “relevant”. Consistent changes in cell population between human physiology and animal model gain value of this model which then can be pharmacodynamically “relevant” from the regulatory point of view. Consequently, the aim of this study was to determine how similar to human observations is the effect of selected biological drugs on blood cells in a pig model. The study was to carry out a comparative analysis of the variability of selected biochemical and hematological parameters of the blood after administration of five different human therapeutic monoclonal antibodies (mAbs) after a single subcutaneous (SC) dose in breeding pigs. The tested drugs were siltuximab (Syl- vant®), omalizumab (Xolair®), infliximab (Inflectra®), pembrolizumab (Keytruda®), and vedoli- zumab (Entyvio®) given in a single 1 mg/kg SC injection. Each of the tested drugs exerted a sig- nificant effect on at least two of the tested parameters three weeks after the administration. Siltuximab significantly influenced 9 of the analyzed parameters. Vedolizumab significantly influenced 8 of the analyzed parameters. Infliximab had the lowest impact of all the tested drugs, as it significantly influenced only two of the analyzed parameters. The study has proved that the impact of mAbs on the analyzed parameters can be significantly extended over time. This requires the monitoring of hematological parameters in the pig model even many weeks af- ter administration of a drug in a relatively small dose.

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Authors and Affiliations

T. Grabowski
A. Burmańczuk
A. Miazek
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Abstract

The aim of the study was to determine biologic variations of the main parameters in cows in early lactation and to compare intra-individual variations of parameters in cows and other species. 50 cows were included in the experiment. Blood samples were taken at the moment of calving, then on 1st, 7th, 14th and 28th day after calving. CVi had the following values: 1.9-5% for MCV, MCH, MCHC, GLU, TProt, ALB, UREA, Ca, P and Mg; 5.1-10% for RBC, Hgb, Hct, WBC, NEU, LYM, BHB, ALT, CHOL, TGC and >10% for PLT, NEFA, TBil, AST and GGT. For RBC, Hgb, Hct, MCV, MCH subject-based reference values or the reference change value should be used. For all other parameters except AST, population-based reference intervals should be used with caution. For LYM, NEU, PLT, GLU, TProt, ALB, CHOL and TGC index of individuality was close to 0.6 which means that subject-based reference values can be much more adequate to use then population-based reference intervals. For AST population-based intervals should be used. There is little effect of parity (increase CVi with parity) and no effect of milk production. CVi values obtained in cows in early lactation are specific because they deviate from the CVi values of other animal species and human. Calculated RCVs varied from 5.5% for MCHC to 41% for AST. High individuality index for many metabolic parameters opens possibility for development of model for longitudinal monitoring of metabolic status of individual animals during lactation. That requires further research.
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Authors and Affiliations

V. Kovačević
1
M.R. Cincović
1
B. Belić
1
R. Đoković
2
I. Lakić
1
M. Radinović
1
A. Potkonjak
1

  1. Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg D.Obradovića 8, 21000 Novi Sad, Serbia
  2. Faculty of Agronomy, University of Kragujevac, Ul. Cara Dušana 34, 32000 Čačak, Serbia
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Abstract

The study was conducted on 26 male, 30 days-old goats, separated from their mothers, divided into two equal groups: I - control and II - experimental, consisting of 13 animals each. All animals were fed with milk replacer, experimental group received additionally 50 g/kg body weight, additive of HMB, for 60 days. The following features were analyzed: body weight, daily increases of body weight, as well as hematological and biochemical blood features. Differences in body weight were found, between experimental and control group, after 60 days of experiment 0.57 kg (p≤0.01). The daily weight gain of experimental animals was higher in comparison with control group. Significant differences were also noted in results of hematological and biochemical blood parameters. Experimental animals showed a higher level of red blood cells as well as number of lymphocytes in comparison with the control group, (p≤0.01).Significant changes were also observed in the level of triglycerides, inorganic phosphorus and protein between both groups. The acid-base balance parameters and ionogram, showed a higher pH level (p≤0.05) HCO – act., HCO – std., BE, ctCO , O sat, K+, Cl– (p≤0.01), while the anion gap (AG) and Na+ were significantly lower in control group (p≤0.01).

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Authors and Affiliations

K. Cebulska
P. Sobiech
S. Milewski
K. Ząbek
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Abstract

This experimental study aimed to investigate some hematological and immunological changes as a result of Aeromanas hydrophila infection in Siberian sturgeon ( Acipenser baerii Brandt, 1869). Their feeds were supplemented with β-1,3/1,6 glucan at different ratios, 250 mg/kg (βG 250); 500 mg/kg (βG 500) and 750 mg/kg (βG 750). To create an experimental infection, 4×106 cfu/ml Aeromonas hydrophila inoculum was intraperitoneally injected to fish. 0.1 ml intraperitoneal bacteria injection was given to the fish in 12 of 15 tanks, each consisting of 10 fish (the fish in the control group were not given bacteria). Considering the βG 500 and βG 750 group fish as positive (C+) and negative control (C−) groups in terms of hematological parameters, it was found that RBC (erythrocyte) and Hb (hemoglobin) values, as well as RBC indices (MCV, MCHC, MCH) significantly increased. The immunological parameters, including WBC (leukocyte), leukocyte cell percentages (lymphocyte, monocyte, neutrophil, eosinophil), as well as cytokines, including IL-1β, IL-6, IL-8, and TNF-α values showed similar increases in the βG 500 and βG 750 groups. It was found that the addition of 500 and 750 mg/kg doses of β-1,3/1,6 glucan to the feed stimulated non-specific immunity of fish against bacterial agents and/or septicemic diseases and beta glucan at this dosage range was determined to be ideal for fish health and that it may be a herbal immunostimulant that can be an alternative to many medicaments.
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Authors and Affiliations

S. Duman
1
A. Şahan
2

  1. Cukurova University, Imamoglu Vocational School, Department of Aquaculture, 01700, İmamoglu, Adana, Turkey
  2. Cukurova University, Fisheries Faculty, Department of Aquaculture, 01330, Balcalı, Adana, Turkey

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