The paper aims to give the results of initial research on the collection of incunabula from the St John’s church in Gdańsk. The collection constitutes significant part of PAN Biblioteka Gdańska. The author reconstructed the size and contents of the collection on the basis of the original church catalogues, marks of ownership and the physical details of the items. The books were gathered in the St John’s church from the time of the collections foundation until the 20th century. The paper establishes also the losses sustained by St John’s church’s library during World War II. Thanks to the incunabula’s content analysis the author determines the thematic focal points of the collection.