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Abstract

The aim of this study was to analyze the chemiluminescence (CL) of peripheral blood in clinically healthy horses of different sexes and ages. The tests were carried out on 119 half- -breed horses, representing various forms of use (66 recreational horses and 53 sport horses). The test material was peripheral blood, which was collected under resting conditions, i.e. before physical activity related to the use of these animals. In the blood samples, spontaneous and stimulated CL with zymosan and phorbol myristate acetate were determined. It has been found that regular training effort increases the blood’s pro-oxidative potential, which was demonstrated by significantly higher (p<0.05) CL values in sport horses compared to recreational animals. Analysis of the results did not show any statistically significant correlation between sex or age of the horses with chemiluminescence values in peripheral blood. The result of the research suggests the need to optimize the results of blood CL measurements, taking into account the number of neutrophils and the concentration of hemoglobin in the blood of tested animals. Analysis of non-optimized blood CL results may lead to premature conclusions.
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Bibliography


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Bedouhène S, Moulti-Mati F, Hurtado-Nedelec M, Dang PM, El-Benna J (2017) Luminol-amplified chemiluminescence detects mainly su-peroxide anion produced by human neutrophils. Am J Blood Res 7: 41-48.

Bereshchenko O, Bruscoli S, Riccardi C (2018) Glucocorticoids, Sex Hormones, and Immunity. Front Immunol 9: 1332.

Burlikowska K, Bogusławska-Tryk M, Szymeczko R, Piotrowska A (2015) Haematological and biochemical blood parameters in horses used for sport and recreation. J Cent Eur Agric 16: 370-382.

Caldefie-Chézet F, Walrand S, Moinard C, Tridon A, Chassagne J, Vasson MP (2002) Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome C reduction. Clin Chim Acta 319: 9–17.

Cywińska A, Wyszyńska Z, Górecka R, Szarska E, Witkowski L, Dziekan P, Winnicka A, Schollenberger A (2010) The effect of the 162 km endurance rideon equine peripheral blood neutrophil and lymphocyte functions. Pol J Vet Sci 13: 279-285.

Cywińska A, Turło A, Witkowski L, Szarska E, Winnicka A (2014) Changes in blood cytokine concentrations in horses after long-distance endurance rides. Med Weter 70: 568-571.

Doucet DR, Bonitz RP, Feinman R, Colorado I, Ramanathan M, Feketeova E, Condon M, Machiedo GW, Hauser CJ, Xu DZ, Deitch EA (2010) Estrogenic hormone modulation abrogates changes in red blood cell deformability and neutrophil activation in trauma hemorrhagic shock. J Trauma 68: 35-41.

Escribano BM, Castejón FM, Vivo R, Santisteban R, Agűera EI, Rubio MD (2005) Effects of training on phagocytic and oxidative metabo-lism of peripheral neutrophils in horses exercised in the aerobic-anaerobic transition area. Vet Res Commun 29: 149-158.

Forslid J, Hed J (1982) In vitro effect of hydrocortisone on the attachment and ingestion phases of immunoglobulin G- and complement com-ponent 3b-mediated phagocytosis by human neutrophils. Infect Immun 38: 811-816.

Frape D (2010) Equine nutrition and feeding. 4th ed., Oxford, UK John Wiley & Sons Ltd, pp 90-179.

Hughes DL, Richards RS, Lexis LA (2018) Using chemiluminescence to determine whole blood antioxidant capacity in rheumatoid arthritis and Parkinson’s disease patients. Luminescence 33: 764-770.

Hyyppä S (2005) Endocrinal responses in exercising horses. Livestock Prod Sci 92: 113-121.

Iranifam M (2014) Analytical applications of chemiluminescence methods for cancer detection and therapy. TrAC Trends in Analytical Chem-istry 59: 156-183.

Jimenez AM, Navas MJ (2002) Chemiluminescence Methods (Present and Future). Grasas y Aceites 53: 64-75.

Klink M, Bednarska K, Blus E, Kiełbik M, Sulowska Z (2012) Seasonal changes in activities of human neutrophils in vitro. Inflamm Res 61: 11-16.

Koenig JB, Hart J, Harris DM, Cruz AM, Bienzle D (2009) Evaluation of endotoxin activity in blood measured via neutrophil chemilumines-cence in healthy horses and horses with colic. Am J Vet Res 70: 1183-1186.

Korhonen PA, Lilius EM, Hyyppä S, Räsänen LA, Pösö AR (2000) Production of reactive oxygen species in neutrophils after repeated bouts of exercise in Standardbred trotters. J Vet Med A Physiol Pathol Clin Med 47: 565-573.

Kraemer WJ, Ratamess NA, Hymer WC, Nindl BC, Fragala MS (2020) Growth Hormone(s), Testosterone, Insulin-Like Growth Factors, and Cortisol: Roles and Integration for Cellular Development and Growth with Exercise. Front Endocrinol (Lausanne) 11: 33.

Krumrych W, Wiśniewski E (2006) Influence of selected stimulators on chemiluminescence of peripheral blood neutrophils in horses. Med Weter 62: 204-206.

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Krumrych W, Gołda R, Gołyński M, Markiewicz H, Buzała M (2018) Effect of physical exercise on cortisol concentration and neutrophil oxygen metabolism in peripheral blood of horses. Ann Anim Sci 18: 53-68.

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Authors and Affiliations

W. Krumrych
1
J. Danek
2
H. Markiewicz
3
M. Gołyński
4

  1. Department of Microbiology and Immunobiology, Kazimierz Wielki University, Powstańców Wlkp. Avenue 10, 85-090, Bydgoszcz, Poland
  2. Department of Biomedical Engineering, UTP University of Science and Technology, prof. S. Kaliskiego Avenue 7, 85-796 Bydgoszcz, Poland
  3. Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Mazowiecka Street 28, 85-082 Bydgoszcz, Poland
  4. Department of Diagnostics and Clinical Sciences, Veterinary Medicine Institute, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska Street 1, 87-100 Toruń, Poland
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Abstract

Videodermoscopic examinations have only recently started to be used in veterinary medicine and usually involve a small group of animals.
The aim of this study was to compare specific dermoscopic parameters in selected areas of the body of Arabian horses in the summer and winter seasons. The research was conducted between 2018-2019. The procedure was performed on the left side of the horse’s body in seven areas: head, neck, chest, flank, rump, mane, and tail. 42 purebred Arabian horses aged 1-25 (median), 39 mares and 3 stallions were qualified for the study. An Italian Video-Dermascope 7 (Medici Medical SRL with the 3 VIDIX 5Mpx camera and the VX1 overlay - Contact type cap Ø 3.5 cm) was used in the dermatoscopic evaluation. Specialist Cellsens Olympus software for analysing microscopic images was used to perform the measurements. Regardless of the area assessed, the skin in the summer months is darker than in the winter months. Hair thickness ranged from 44.82 (chest in summer) to 87.45 um (mane in winter). Regarding hair density, the number of hairs in the field of view of the dermatoscope ranged from 990 (summer mane) to 3680 (head in winter). Our research showed that the season of the year influences the amount of hair without significantly affecting its thickness.
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Authors and Affiliations

A. Pomorska-Zniszczyńska
1
M.P. Szczepanik
2
G. Kalisz
3
N. Adamczyk
4
M. Tarach
4
M. Sobuś
4
B. Abramowicz
5

  1. Subdepartment of Internal Diseases of Farm Animals and Horses, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
  2. Subdepartment of Clinical Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
  3. Department of Biopharmacy, Faculty of Pharmacy, Medical University of Lublin, Lublin, Poland
  4. Students, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland
  5. Sub-Department of Internal Diseases of Accompanying Animals, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
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Abstract

Parasitosis in horses may be uncontrolled and expose breeders and owners to serious financial losses or, possibly, to the loss of animals. Therefore, the prevention and monitoring of the development of parasitic diseases should play an important role in the breeding process. The aim of this study was to confirm the influence of factors such as age, breed, herd size, deworming program, and type of anthelmintics, on the prevalence and intensity of parasites (helminths) in domestic horses in Lower Silesia. The study was carried out between August and November of 2020. The samples of horse feces were collected from 50 different stables in the area of Lower Silesia, Poland. A total of 286 individuals from various breeds were examined. Detailed analysis revealed that the mean age of infected horses was significantly lower than in uninfected horses. The mean time since the last deworming procedure was almost twice as low in uninfected horses than in infected ones. Additionally, the deworming agent affects the prevalence of infection. The analysis was also performed for the same factors in reference to quantitative data. The mean EPG was four-fold higher in juvenile horses than in adults and three-fold higher when the horses were dewormed with the use of fenbendazole instead of ivermectin or ivermectin with praziquantel combined.
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Authors and Affiliations

W. Hildebrand
1
P. Zielińska
2
J. Hildebrand
3
G. Zaleśny
4

  1. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland
  2. Department of Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 51, 50-366 Wroclaw, Poland
  3. Department of Parasitology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63, Wrocław, 51-148, Poland
  4. Department of Invertebrate Systematics and Ecology, Institute of Environmental Biology, Wroclaw University of Environmental and Life Sciences, Kożuchowska 5B, 51-631 Wroclaw, Poland
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Abstract

Resistance of cyathostomins to benzimidazole (BZ) anthelmintics is widespread in horses in many parts of the world. This study compared three methods for the determination of benzimidazole resistance of Cyathostominae in 18 horses from a stud farm in Romania. The horses were treated with Fenbendazole. The resistance test was performed by FECRT, ERP and PCR. On Day 0, larvae of species belonging to the Cyathostominae subfamily, types A, B, C, D and Gyalocephalus, as well as Strongylus vulgaris species of the Strongylinae subfamily, were identified. At 42 days post treatment with fenbendazole only larvae of Cyathostominae, types A and D were identified. Resistance to Fenbendazole was found in one horse, using the FECRT and ERP tests. Both genetic resistance and susceptibility to BZ anthelmintics was observed in 13 samples (72.22%) using the PCR test. However, three samples (16.67%) showed only the BZ-susceptibility gene. In 2 samples, (11.11%) only the resistance gene to BZ anthelmintics was identified. Several inconsistencies in the evidence of resistance to benzimidazole were observed between the PCR test and the other two methods, which indicates that several methods for determining and controlling the resistance should be used in practice.
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Authors and Affiliations

G. Dărăbuş
1
C. Badea
1
I. Oprescu
1
S. Morariu
1
N. Mederle
1
M. Ilie
1
T. Suici
1
M. Imre
1

  1. Banat University of Agricultural Sciences and Veterinary Medicine of Timişoara, Faculty of Veterinary Medicine, 300645, Calea Aradului no. 119, Timişoara, Romania
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Abstract

Observations on the development of the horse chestnut leafminer on red horse chestnut (Aesculus x carnea H.) were carried out in Wrocław, Lower Silesia, Poland, in 2001–2003. Three generations of the pest were recorded to lay eggs on the red horse chestnut leaves. Although females of each generation deposited eggs abundantly, the hatching larvae died after a short period of feeding in the plant’s leaves and the species did not complete its development on this tree. Mostly L1 and L2 larvae were found inside the leaf mines. The observed leaf damage was, therefore, negligible.

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Authors and Affiliations

Agnieszka Kukuła-Młynarczyk
Michał Hurej
Jacek Jackowski
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Abstract

The effect of Ageratina adenophora on pathological characteristics of the liver and lungs as well as serum biochemical parameters in horses were investigated. Ten horses without ingestion history of Ageratina adenophora were classified into the control group, and 10 poisoned but survived horses with 3 months ingestion history were set as the case group. Results showed that serum AST, ALT, ALP, magnesium and phosphorus were elevated significantly, while creatinine was decreased remarkably. Hematoxylin and eosin staining of liver tissues showed diffuse swelling or destruction of hepatocytes, narrowing or atrophy of the hepatic sinusoids, and little lymphocytic infiltration; lung tissues presented destroyed alveoli and inflammatory cell infiltration.
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Authors and Affiliations

X.L. Gu
1
F.Y. Dai
1
X. Xiao
1
G.Z. Li
2
L.M. Zhang
1
W.J. Qu
1

  1. College of Veterinary Medicine, Yunnan Agricultural University, Jin Hei Road No.65, Panlong District, 650051, Kunming, P.R. China
  2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P.R. China
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Abstract

The aim of this study was to evaluate the influence of training on body surface temperature over the joints in racehorses, measured by infrared thermography. The study involved monitoring of 14 Thoroughbred racehorses in 6 imaging sessions over a period of 3 months. Temperature measurements of the forelimb and hindlimb joints were made before and just after training. Joint temperature of limbs increased significantly after training. Environmental temperature had a statistically significant influence on surface temperature over the joints. The lowest surface temperatures were recorded over the metacarpophalangeal and metatarsophalangeal joint and the highest temperatures in the shoulder, elbow, hip and stifle joint. The metacarpophalangeal and metatarsophalangeal joints warmed the least during training, but were influenced the most by differences in environmental temperature. The surface temperature difference before and after training is an important indicator of the thermoregulatory response to exercise in racing horses. Understanding surface temperature changes in response to regular training is necessary for future studies on diagnosing injuries of joints.
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Bibliography

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Authors and Affiliations

M. Soroko
1
W. Górniak
2
M. Godlewska
1
K. Howell
3

  1. Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland
  2. Department of Automotive Engineering, Wroclaw University of Science and Technology, Na Grobli 13, 50-421 Wroclaw, Poland
  3. Microvascular Diagnostics, Institute of Immunity and Transplantation, Royal Free Hospital, Pond Street, London NW3 2QG, UK
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Abstract

Asthma is one of the most common non-infectious respiratory diseases in horses. Ultrasound examination is a widely available non-invasive additional diagnostic tool. To date, there are no studies focusing on ultrasonographic findings in horses with asthma. The aim of this study was to analyse the prevalence and severity of ultrasound lesions in lung tissue in horses with asthma. Lung ultrasonography was carried out on six healthy horses (controls) and 12 horses with asthma (six with mild and six with severe asthma). The sonographic changes in three lung sections were assessed using a scoring system. The most common changes present in all the animals were comet- tail artefacts. More advanced lesions were present in horses with severe asthma. Statistically significant differences in the overall average intensity of the ultrasound changes were seen between the controls and the study group and between the horses with mild and severe asthma. The lesions were usually located in the caudal lung regions, but they were also present in other areas as the disease progressed. Ultrasonography is a useful additional diagnostic tool enabling an assessment of the stage of the asthma progression. It is a very sensitive technique that visualizes minor lesions in the lung tissue even in clinically healthy animals. Due to its low specificity, it cannot replace endoscopy and the bronchoalveolar lavage in horses with asthma.

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Authors and Affiliations

N. Siwinska
A. Zak
M. Slowikowska
P. Krupinska
A. Niedzwiedz
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Abstract

Observations on the incidence, harmfulness and some elements of Cameraria ohridella control on white horse chestnut (Aesculus hippocastanum L.) were carried out in Wrocław, Poland, in 2001–2003. Three generations of the pest developed on A. hippocastanum. The leaf infestation by pest’s larvae increased systematically throughout the spring-summer season. Cultural control by removing the fallen leaves was suggested. Using this procedure, the abundance of the pest was considerably reduced, therefore, the percentage of the leaf damage was lower and the trees lost these leaves later in the season.

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Authors and Affiliations

Agnieszka Kukuła-Młynarczyk
Michał Hurej
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Abstract

Desmopathy of the collateral ligaments of the equine interphalangeal joint is caused by a combination of factors, including hoof shape, excessive loading and ground surface. This complex problem poses a diagnostic challenge due to the non-specificity of perineural analgesia and the limitations associated with the most popular imaging methods such as radiography and ultrasonography. The aim of this study was to retrospectively determine the prevalence of desmopathy of the collateral ligaments of the equine distal interphalangeal joint in Poland between 2016 and 2019, and to establish the frequency and type of the associated pathologies. Desmopathy of the collateral ligaments of the distal interphalangeal joint was diagnosed in 14% of 152 horses examined by magnetic resonance imaging (MRI). In 64% of the cases, other changes were observed in the equine digit, and in 36% of the cases, desmopathy was the only diagnosed problem. Desmopathy of the collateral ligaments is not a frequently reported pathology in the distal part of the equine limb. The diagnostic difficulties described in this article suggest that considerable caution should be exercised when formulating the final diagnosis, prognosis and treatment options.
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Authors and Affiliations

M. Mieszkowska
1
J. Abako
1
M. Mieszkowski
2
Y. Zhalniarovich
1

  1. Department of Surgery and Radiology with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, Olsztyn, 10-719, Poland
  2. Department of Anesthesiology and Intensive Care, Faculty of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, al. Warszawska 30, 10-082, Olsztyn, Poland
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Abstract

The aim of this study was to evaluate the influence of the newly isolated Lactobacillus plantarum LUHS135 and Lactobacillus paracasei LUHS244 strains grown in potato juice (with a cell count of 8.0-9.0 log10 CFU/ml) on the blood and faeces parameters of exercising horses. The horses were classified into four different groups: a control group (which received no probiotics); the first group (which received 200 ml of L. plantarum culture in potato juice); the second group (which received 200 ml of L. paracasei culture in potato juice); and the third group (which received an L. plantarum and L. paracasei mix (with the mix consisting of 100 ml of each). Indices for the blood and faeces microflora were obtained before and after treatment of horses (on days zero and thirty). It was observed that the count for lactic acid bacteria (LAB) in the faeces was significantly higher on day thirty, whereas it was lower when it came to the total enterobacteria count (TCE). Despite the ambiguous influence of any treatment on blood parameters, the L. plantarum × L. paracasei mixture increased the concentration of HGB and O2 saturation in blood samples which were taken from the horses. L. paracasei significantly decreased the lactate concentration levels in horse blood samples. As a result of the present study, it can clearly be seen that the strains being used revealed their potential application as probiotics; however, further studies are required to prove the survival and action mechanisms of the newly isolated strains.

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Authors and Affiliations

P. Zavistanaviciute
I. Poskiene
V. Lele
R. Antanaitis
J. Kantautaite
E. Bartkiene
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Abstract

Trypanosomiasis is one of the severe pathogenic infections, caused by several Trypanosoma species, affecting both animals and humans, causing substantial economic losses and severe illness. The objective of this study was to determine the molecular diagnosis and the risk factors associated with trypanosomiasis in District Jhang, Punjab, Pakistan. For this purpose, blood samples were randomly collected from 200 horses. A predesigned questionnaire was used to collect data on risk factors before the sample collection. The microscopy examination through Giemsa staining, formol gel test and PCR techniques were used to find the prevalence. The prevalence was recorded as 22.5% with microscopy examination, 21% through formol gel test and 15.5% with PCR based results. Analysis of risk factors associated with Trypanosoma brucei evansi occurrence was carried out using Chi-square test. It showed the prevalence of Trypanosoma brucei evansi was significantly (p<0.05) associated with sex, age, rearing purpose and body condition whereas non-significantly (p>0.05) with insects control practices. This study supports the idea that PCR is a sensitive, robust and more reliable technique to diagnose trypanosomiasis. It was concluded that Trypanosoma brucei evansi is widely prevalent in Jhang (Pakistan), highlighting a dire need to develop control strategies and education programmes to control this disease in developing countries.
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Authors and Affiliations

J. Zahoor
1
M. Kashif
1
A. Nasir
1
M. Bakhsh
1
M.F. Qamar
2
A. Sikandar
3
A. Rehman
2

  1. Department of Clinical Medicine, College of Veterinary and Animal Sciences, Jhang, Pakistan
  2. Department of Pathobiology, College of Veterinary and Animal Sciences, Jhang Pakistan
  3. Department of Basic Sciences, College of Veterinary and Animal Sciences, Jhang Pakistan
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Abstract

The aim of this study was to assess sand accumulation in the gastrointestinal tract and fecal sand excretion in Silesian foals using three diagnostic methods and taking into account the sex and age of the animals. Another aim of the study was to compare the three diagnostic methods. The study was carried out on 21 clinically healthy Silesian foals (10 females and 11 males) from 9-28 weeks old grazed on permanent pasture. The sand intake was assessed using a sedimentation test, abdominal ultrasonography and a quantitative evaluation of sand per 100 g of stool. In the sedimentation test, the sand was palpable in the stool of 57.1% of the horses, and clearly visible in 42.9% of the animals. The ultrasound examination revealed the presence of sand in the gastrointestinal tract in 66.7% of the horses. It was limited to a single location in 60% of the horses, while it was present in several regions in 40% of the horses. The mean amount of sand was 0.14 ± 0.33 g per 100 g of stool. It did not exceed 0.1g in 71.4% foals, while it ranged from 0.1-0.5 g in 23.8% foals. In 4.8% of the animals, it amounted to 1.6 g per 100 g of stool. There was no correlation between age and gender and the results. There was a positive correlation between the ultrasound examination and the sedimentation test.

Sand may be accumulated in the gastrointestinal tract of foals without any clinical signs. The amount of sand excreted in the stool is not an indicator of the amount of sand accumulated in the gastrointestinal tract. An abdominal ultrasound examination should be combined with a sedimentation test for more specific results.

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Authors and Affiliations

N. Siwińska
A. Łuczka
A. Żak
M. Słowikowska
A. Niedźwiedź
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Abstract

The chronic degenerative endometritis (CDE) is recognised as directly related to age and infertility in the mare. In this study, the 14 days post ovulation (PO) pregnancy rate was assessed in 60 barren mares affected by CDE, submitted to platelet-rich-plasma intrauterine infusion (PRPI) 24 hours PO. Data showed a significant positive effect of PRPI on the chance to become pregnant in mares affected by Kenney I-II CDE. The overall 14 days PO pregnancy rate was 75%. Sixty-nine % of the overall pregnancies was achieved with the 1st PRPI, but an additional 31% was obtained with the 2nd PRPI at the subsequent heat, increasing significantly the number of pregnancies. Although the 1st PRPI lead to a higher pregnancy rate than the 2nd PRPI (52 vs 23%), the repetition of the treatment had a significant positive effect on the overall pregnancies. Mare’s young age positively influenced the chance to become pregnant and was associated to endometritis Kenney I. Mares with endometritis Kenney I had better chance to become pregnant than those with endometritis Kenney II. The PRPI proved, therefore, to be beneficial in barren mares with CDE, and resulted in high overall pregnancy rate achievement in barren mares.

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Authors and Affiliations

A. Carluccio
M.C. Veronesi
D. Plenteda
A. Mazzatenta

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