Currently, the distribution system has been adapted to include a variety of Distributed Energy Resources (DERs). Maximum benefits can be extracted from the distribution system with high penetration of DERs by transforming it into a sustainable, isolated microgrid. The key aspects to be addressed for this transformation are the determination of the slack bus and assurance of reliable supply to the prioritized loads even during contingency. This paper explores the possibilities of transforming the existing distribution system into a sustainable isolated network by determining the slack bus and the optimal locations and capacity of Distributed Generators (DGs) in the isolated network, taking into account the contingencies due to faults in the network. A combined sensitivity index is formulated to determine the most sensitive buses for DG placement. Further, the reliability based on the loss of load in the isolated system when a fault occurs is evaluated, and the modifications required in for reliability improvement are discussed. The supremacy of the transformed isolated network with distributed generators is comprehended by comparing the results from conventional IEEE 33-bus grid connected test system and modified IEEE 33-bus isolated test system having no interconnection with the main grid.
First psychological research at Arctowski station were conducted in 1979. In the nineties the American team under direction of prof. L. Palinkas conducted research in order to determine the patterns of multicultural psychosocial adaptation. The author discusses stress as a result of isolation and extreme conditions.
In this fast-changing environmental condition, the effect of fossil fuel in vehicle is a significant concern. Many sustainable sources are being studied to replace the exhausting fossil fuel in most of the countries. This paper surveys the types of electric vehicle’s energy sources and current scenario of the onroad electric vehicle and its technical challenges. It summarizes the number of state-of-the-art research progresses in bidirectional dcdc converters and its control strategies reported in last two decades. The performance of the various topologies of bidirectional dc-dc converters is also tabulated along with their references. Hence, this work will present a clear view on the development of state-of-the-art topologies in bidirectional dc-dc converters. This review paper will be a guide for the researchers for selecting suitable bidirectional traction dc-dc converters for electric vehicle and it gives the clear picture of this research field.
Considering concrete nonlinearity, the wave height limit between small and large amplitude sloshing is defined based on the Bernoulli equation. Based on Navier-Stokes equations, the mathematical model of large amplitude sloshing is established for a Concrete Rectangle Liquid-Storage Structure (CRLSS). The results show that the seismic response of a CRLSS increases with the increase of seismic intensity. Under different seismic fortification intensities, the change in trend of wave height, wallboard displacement, and stress are the same, but the amplitudes are not. The areas of stress concentration appear mainly at the connections between the wallboards, and the connections between the wallboard and the bottom.
Stem canker and black scurf of potato (Solanum tuberosum L.) caused by Rhizoctonia solani Kühn are important and epidemic diseases in potato-growing regions worldwide, including Iran. In this study, 120 isolates were retrieved from infected stem canker from six potato- growing regions in Iran (Isfahan, Ardebil, Fars, Hamedan, Kurdestan and Kerman). Out of these, 30 isolates were selected as representatives for genetic and virulence analysis. The isolates were analyzed by one sequence analyzes of the ITS-rDNA region, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), as well as virulence studies. Based on sequence analysis of the ITS-rDNA region, all 30 isolates were assigned to the anastomosis group (AG) and all were assigned to AG-3 PT. Cluster analysis using the unweighted pair group method with the arithmetic averages (UPGMA) method for both RAPD and ISSR markers revealed that they were divided into three main groups, with no correlation to geographical regions of the isolates. Pathogenicity tests showed that all isolates were pathogenic on potato cv. Agria; however, virulence variability was observed among the isolates. The grouping based on RAPD analysis and virulence variability was not correlated.
RNA extraction involves several main stages, regardless of the method of extraction: homogenization, effective denaturation of proteins from RNA, inactivation of ribonuclease and removal of any DNA, protein, and some residual contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of polysaccharides and phenols. Several efforts have been made towards the comparison and optimization of extraction and purification methods for RNA from plant tissues. This is dictated by the necessity of obtaining RNA of a good quality and in a sufficient quantity for further molecular analyzes. Plant storage organs (such as bulbs or seeds) rich in polysaccharide and polyphenolic compounds present distinct challenges for total RNA isolation. Such components, considered in this case as contamination, may bind and co-precipitate with nucleic acids and negatively affect later assays. Since standard routine protocols yield unacceptable results in bulbs, we have designed a new method for RNA extraction. We used two modified procedures (based on CTAB and sarkosyl reagents) of RNA extraction from so called “difficult plant material” and compared them to a popular RNA isolation base on the column isolation kit and TriPure reagent. Our modified protocols dealt with problems of both RNA degradation and low yield caused by co-purification with polysaccharides present in plant bulbs. In this study we have shown that improvement of the CTAB and sarkosyl method with a lyophilization step of plant tissues leads to isolation of high quality RNA from difficult material like storage organs of bulbous plants. The main changes in the procedure compared to the previously described methods concerned the different order of lithium chloride and sodium acetate addition, lithium chloride concentration increase and modification of centrifugation conditions. Gel electrophoresis and spectrophotometer analysis confirmed the high quality and integrity of the obtained RNA. The modified procedures allowed for obtaining a satisfying amount of RNA concentration in the range from 280 to 950 ng/μl depending on the plant species. Thus, the demonstrated RNA isolation methods are efficient and can be used for plant material rich in polysaccharides, such as bulbs.