Investigation of leaf rust disease on spring crops of triticale (× Triticosecale Wittm.), it sdi stribution dynamics, as well as the efficacy of two fungicides, different application doses and timing was carried out at the Lithuanian Institute of Agriculture during 2000–2002. Differences in the disease development were determined by the meteorological conditions, especially the amount of rainfall, and growth stage of plants. Precipitation during 2000 was close to the mean, and the development of leaf rust was moderate. In June of 2001 the rainfall was twice as high as the norm, which created favourable conditions for pathogen development. In 2002 a long droughty period till flowering inhibited the development of leaf rust. The triazole fungicides Juventus (metkonazole 60 g l^–1) and triazole and strobilurine mixture. Allegro (kresoxim-methyl 125 g l^–1 + epoxikonazole 125 g l^–1) were used at full, two-third and half doses once and twice. Both of the fungicides were very effective against leaf rust. Biological efficacy of Juventus applied at any dose or time against leaf rust was 88.3%–99.7%. Allegro efficacy against this disease was slightly higher 94.7%–100%. Application of 1.0; 0.75 and 0.5 l ha^–1 doses twice showed a better efficacy than a single application. The higher doses of fungicides were not markedly superior to the lower ones.

Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field resistance against fungal pathogens is a priority of many breeding programs. This type of resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages. Until now, seven genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr75, Lr77 and Lr78) conferring durable types of resistance against multiple fungal pathogens have been identified in the wheat gene pool. In this study we showed a multiplex Polymerase Chain Reaction (multiplex PCR) assay, which was developed for detection of slow rusting resistance genes Lr34, Lr46, Lr68, using molecular markers: csLV34, Xwmc44 and csGS, respectively. Identification of molecular markers was performed on 40 selected wheat genotypes which are the sources of slow rusting genes according to literature reports. Multiplex PCR is an important tool to reduce the time and cost of analysis. This multiplex PCR protocol can be applicable for genotyping processes and marker assisted resistance breeding of wheat.

The resistance of winter wheat varieties to Puccinia recondite f. sp. tritici was investigated at the Lithuanian Institute of Agriculture during 2001–2003. Effectiveness of resistance genes was investigated at seedling, tillering and adult plant stages. Virulence tests done during the 2000–2003 period showed that the majority of Lr genes used in European wheat were not sufficiently efficient. However, testing of cultivars at the first leaf stage revealed that the Lr37 gene in combination with the other genes was very effective. The experimental cultivars were sown in 2 times: in autumn and spring, without vernalization. The main task of spring-sown nursery was to improve the effectiveness of the experiment and investigate the effect of different Lr genes of non-vernalized plants at tillering growth stage. The Lr37 gene was found to be the most effective at both adult plant stage and tillering growth stage. Disease severity and plant resistance type at tillering stage were stable in all experimental years, which is important for the breeding program. The investigations revealed that the correlations between resistance at seedling and the other two stages were up to r = 0.81 (significant at p = 0.01**). The correlations between leaf rust severity and varietal resistance type at tillering were very high (r = 0.86–0.91**) in the same year. The correlation of leaf rust severity at adult plant stage was strong (r = 0.78**) between 2001 and 2002, but too low for reliable selection of resistant cultivars in the other years. Spring-sown nursery was complementary to collect resistance data in the years unfavourable for leaf rust development.