In 1995- 1997 and 1998-1999 floristical investigations were carried out within the projected ,,Dolina Sztoły" Landscape Protected Area, located in the Olkusz district (north-eastern part of the Silesian Upland). The occurrence of 413 taxa of vascular plants was reported. Out of 23 legally protected species, 16 are strictly and 7 are partly protected. Among vascular plants there are 28 regionally rare and endangered taxa, Medicinal materials used in Polish oflicinal medicine can be derived from 46 species. Eleven species (2,6% of vascular flora) represent mountain element. The richest group are hemicryptophytes (49,4%), while woody chamaephytes are represented by 3,4% of species.

We determined the level of flavonoids, citric acid and ascorbic acid in hips of rose species from the Caninae section occurring in Poland. We performed phytochemical analyses of 75 samples representing 11 species: Rosaagrestis Savi, R. canina L., R. dumalis Bechst., R. glauca Pourret, R. inodora Fries, R. jundzillii Besser, R. rubiginosa L., R. sherardii Davies, R. tomentosa Sm., R. villosa L. and R. zalana Wiesb. Flavonoid content was determined spectrophotometrically, and organic acid concentrations by HPLC. The content of the studied compounds varied greatly. Interspecific differences in the amount of flavonoids and ascorbic acid were highly significant. The most common species, Rosa canina, showed low average content of vitamin C (0.51 g/100 g of dry matter) and flavonoids (41 mg/100 g DM) and high content of citric acid (3.48 g/100 g DM). Ascorbic acid was highest in R. villosa hips (avg. 2.25 g/100 g DM), flavonoids were highest in R. rubiginosa (72 mg/100 g DM), and citric acid was highest in R. tomentosa (4.34 g/100 g DM). Flavonoid level correlated negatively with the amount of citric acid (r=-0.47, p<0.001). Cluster analysis of rose species based on the content of the investigated compounds confirmed the validity of the division of sect. Caninae into three subsections: Rubiginosae, Vestitae and Rubrifoliae. The phytochemical variation of these roses reflects their probable phylogenetic relationships as determined from morphology.

We explored the use of the medicinally important plant Centella asiatica for expression of hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) strain AF2240. HN protein is the principal target for subunit vaccine development against NDV. The full-length HN gene was cloned into a plant expression construct driven by the CaMV 35S promoter and C-terminal fusion of green fluorescence protein (GFP) as reporter system. The recombinant expression construct was transformed via particle bombardment into C. asiatica callus. Transformants were screened using GFP and selected on MS medium supplemented with 15 mg/l hygromycin. The ~1.8 kb HN mRNA transcript was detected on the putative transformants using RT-PCR. The presence of HN protein expression was further confirmed through dot blot analysis using anti-NDV chicken serum. Here we report, for the first time, the use of a novel medicinal plant as a new platform for HN protein expression.

Septoria melissae Desm., the most important pathogen of lemon balm (Melissa officinalis) occurs each year on plantations. The fungus may cause serious yield losses in the absence of proper plant protection. Breeding resistant or tolerant cultivars could play an important role in plant protection of medicinal plants. However, only a few descriptions of tolerant varieties of lemon balm are available. The goal of this work was to evaluate the susceptibility of three accessions of M. officinalis against the pathogen of Septoria leaf spot under field conditions at Budapest-Soroksár (Hungary) in 2017–2018. Differences in susceptibility of the accessions were observed in both years. The accession of M. officinalis subsp. altissima proved to be the least susceptible to Septoria infection. The frequency of the infected leaves was only 5.1 and 28.1% in 2017 and 2018, respectively. However, the cultivar M. officinalis subsp. officinalis ‘Lorelei’ turned out to be the most susceptible to the pathogen with an average infection level of 26.1 and 66.6%, 1.3–6.1 times higher than that of the other accessions in each year, respectively. Development of disease tolerant M. officinalis cultivars may be an effective tool in the plant protection of lemon balm.

In the current study, it was tried to investigate the medicinal plants of the native flora of the Northern Tien Shan to bring them into domestication. The study was carried out on the territory of a botanical garden located at an altitude of 880 m a.s.l., in the foothill steppe zone of the Zailiysky Alatau ridge, People’s Republic of China on light chestnut loamy soils. In 2018–2019, more than 90 samples of 51 species of medicinal plants of the Northern Tien Shan flora was selected from 17 families for introduction tests. The families Compositae (10 genera, 12 species) and Lamiaceae (12 genera, 13 species) were represented by the largest numbers of genera and species. The family Leguminosae was represented by four species from four genera; the families Polygonaceae and Ranunculaceae, by three species each; the family Rosaceae, by two species; and the remaining 11 families, by one species each. The results suggested that the majority of medicinal plants of the Northern Tien Shan tested can be successfully cultivated in the foothill zone of the Zailiysky Alatau. The results indicated that when propagated by seed, the laboratory germination varied from 2 to 30%, and the mass of seeds was 0.21 g. When sown in spring, the field germination was 8%, and when sown in autumn, 42% from 50 to 70% of seedlings survived until the end of the growing season. The massive flowering was observed in the third year, and the plants vegetated until the end of the growing season.

The goal of this study was to evaluate the effectiveness of aqueous extracts from five traditional Egyptian medicinal plants in preventing Sphaerotheca fuliginea’s powdery mildew disease, which affects cucumber plants. Aqueous extracts from each of the examined plants suppressed the pathogen’s conidia germination in vitro. In trials using detached leaves and greenhouses, these extracts lessened the severity of the disease. Compared to other plant extracts, Curcuma longa rhizome extract showed the greatest potency against the pathogen. The aqueous extract of Curcuma longa showed the largest improvement in disease suppression compared to the control in the greenhouse experiment. The results showed that total phenol and associated defense enzyme levels (POD and PPO) were elevated by plant extracts from all studied plants. These findings might suggest that total phenol and associated defense enzymes strengthen the cucumber’s resistance to the disease. The C. longa extract had more total phenol than the extracts from the other plants. The phenolic components in the C. longa rhizome extract were varied, and these variations were detected and quantified using high-performance liquid chromatography (HPLC). The content of curcumin (3220.8 μg · g ^–1 dry weight) was the highest. In comparison to the control, the foliar application of the C. longa extract considerably increased the cucumber fruit yield and its constituent parts. This is the first time, to my knowledge, that the C. longa rhizome extract has been utilized to improve cucumber plants’ production and its constituent parts. The pathogen appeared as small colonies with fewer mycelia and immature conidia in the treated cucumber leaves with 20% of C. longa rhizome extract according to an examination by SEM. Overall, the results indicated that the extract of C. longa rhizome, was a promising, effective, and environmentally friendly management measure against powdery mildew disease of cucumbers, and thus could be used in the production of organically grown vegetables.