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Towards microfluidics for mycology – material and technological studies on LOCs as new tools ensuring investigation of microscopic fungi and soil organisms

Agnieszka Podwin Tymon Janisz Katarzyna Patejuk Piotr Szyszka Rafał Walczak Jan Dziuban
Bulletin of the Polish Academy of Sciences Technical Sciences | 2021 | 69 | No. 1 | e136212 | DOI: 10.24425/bpasts.2020.136212
Keywords microfluidics lab-chip biomaterials cell cultivation mycology
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Abstract

This paper presents material and technological studies on lab-on-chip (LOC) devices as a first step towards biocompatible and reliable research on microscopic fungi and soil organisms on a microscale. This approach is intended to respond to the growing need for environmental control and protection, by means of modern, miniaturized, portable and dependable microfluidics instrumentation. The authors have presented herein long-term, successful cultivation of different fungi representatives (with emphasis put on Cladosporium macrocarpum) in specially fabricated all-glass LOCs. Notable differences were noted in the development of these creatures on polymer, polydimethylosiloxane (PDMS) cultivation substrates, revealing the uncommon morphological character of the fungi mycelium. The utility of all-glass LOCs was verified for other fungi representatives as well –  Fusarium culmorum and Pencilium expansum, showing technical correspondence and biocompatibility of the devices. On that basis, other future applications of the solution are possible, covering, e.g. investigation of additional, environmentally relevant fungi species. Further development of the LOC instrumentation is also taken into consideration, which could be used for cultivation of other soil organisms and study of their mutual relationships within the integrated microfluidic device.
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Bibliography

  1.  C. Wagg, et al., “Fungal-bacterial diversity and microbiome complexity predict ecosystem functioning”, Nat. Commun. 10, 4841 (2019), doi: 10.1038/s41467-019-12798-y.
  2.  M.J. Roossinck, “Evolutionary and ecological links between plant and fungal viruses”, New Phytol. 221(1), 86‒92 (2019), doi: 10.1111/ nph.15364, 2019.
  3.  H. Grossart, et al., “Fungi in aquatic ecosystems”, Nat. Rev. Microbiol. 17, 339–354 (2019), doi: 10.1038/s41579-019-0175-8.
  4.  M. Rai and G. Agarkar, “Plant–fungal interactions: What triggers the fungi to switch among lifestyles?”, Crit. Rev. Microbiol. 42(3), 428‒38 (2016), doi: 10.3109/1040841X.2014.958052.
  5.  A. Frew, J.R. Powell, G. Glauser, A.E. Bennett, and S.N. Johnson, “Mycorrhizal fungi enhance nutrient uptake but disarm defences in plant roots, promoting plant-parasitic nematode populations”, Soil Biol. Biochem. 126, 123‒132 (2018), doi: 10.1016/j.soilbio.2018.08.019.
  6.  M. Dicke, A. Cusumano, and E.H. Poelman, “Microbial Symbionts of Parasitoids”, Annu. Rev. Entomol. 65, 171‒190 (2020).
  7.  B. Kendrick, The fifth kingdom. An Introduction to mycology. Hackett Publishing Company, Inc., Indianapolis, USA, 2017.
  8.  T.A. Richards, G. Leonard, and J.G. Wideman. “What Defines the “Kingdom” Fungi?” in The fungal Kingdom, Washington, DC: ASM Press, American Society for Microbiology, Wiley Online Library, 2018.
  9.  T.S. Kaminski, O. Scheler, and P. Garstecki, “Droplet microfluidics for microbiology: techniques, applications and challenges”, Lab Chip 16, 2168‒2187 (2016).
  10.  A. Burmeister and A. Grünberger, “Microfluidic cultivation and analysis tools for interaction studies of microbial co-cultures”, Curr. Opin. Biotechnol. 62, 106‒115 (2020).
  11.  C.E. Stanley and M.G.A. van der Heijden, “Microbiome-on-a-Chip: New Frontiers in Plant–Microbiota Research”, Trends Microbiol. 25(8), 610‒613, 2017.
  12.  S.R. Lockery, et al., “Artificial Dirt: Microfluidic Substrates for Nematode Neurobiology and Behavior”, J. Neurophysiol 99(6), 3136‒3143, 2008.
  13.  H. Massalha, E. Korenblum, S. Malitsky, O.H. Shapiro, and A. Aharoni, “Live imaging of root–bacteria interactions in a microfluidics setup”, PNAS 114(17), 4549‒4554 (2017).
  14.  C.S. Effenhauser, A. Paulus, A. Manz, and H.M. Widmer, “High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device”, Anal. Chem. 66, 1994(18), 2949–2953 (1994).
  15.  L.J. Golonka, “Technology and applications of Low Temperature Cofired Ceramic (LTCC) based sensors and microsystems”, Bull. Pol. Ac.: Tech. 54(2), 221‒231 (2006).
  16.  M. Boyd-Moss, S. Baratchi, M. Di Venere, and K. Khoshmanesh, “Self-contained microfluidic systems: A review”, Lab Chip 16(17), 3177‒3192 (2016).
  17.  G.M. Whitesides, “The origins and the future of microfluidics”, Nature 442, 368–373 (2006).
  18.  B. Zhang, M. Kim, T. Thorsen, and Z. Wang, “A self-contained microfluidic cell culture system”, Biomed. Microdevices 11(6), 1233‒1237 (2009).
  19.  S. Ye and I.N.M. Day, Microarrays & microplates: applications in biomedical sciences, 1st Edition, Garland Science, New York, USA, 2002.
  20.  R.J. Courcol, H. Deleersnyder, M. Roussel-Delvallez, and G.R. Martin, “Automated reading of a microtitre plate: preliminary evaluation in antimicrobial susceptibility tests and Enterobacteriaceae identification”, J. Clin. Pathol. 36(3), 341–344 (1983).
  21.  J.H. Platt, A.B. Shore, A.M. Smithyman, and G.L. Kampfner, “A computerised ELISA system for the determination of total and antigen- specific immunoglobulins in serum and secretions”, J. Immunoassay Immunochem. 2, 59‒74 (2006).
  22.  L. Maresová and H. Sychrova, “Applications of a microplate reader in yeast physiology research”, BioTechniques 43(5), 667‒672, 2007.
  23.  M. Frąc, A. Gryta, K. Oszust and N. Kotowicz, “Fast and accurate microplate method (Biolog MT2) for detection of Fusarium fungicides resistance/sensitivity”, Front. Microbiol. 7, 489 (2016).
  24.  C.E. Stanley, G. Grossmann, X. Casadevall i Solvas, and A.J. deMello, “Soil-on-a-Chip: microfluidic platforms for environmental organismal studies”, Lab Chip 16 (2), 228‒241 (2016).
  25.  A. Sanati Nezhad, “Microfluidic platforms for plant cells studies”, Lab Chip 14(17), 3262‒3274 (2014).
  26.  J.C. Jokerst and J.M. Emory, C.S. Henry, “Advances in microf luidics for environmental analysis”, Analyst 137(1), 24‒34 (2012).
  27.  D.W. Inglis, N. Herman, and G. Vesey, “Highly accurate deterministic lateral displacement device and its application to purification of fungal spores”, Biomicrofluidics 24(2), 024109 (2010).
  28.  Z. Palková, L. Váchová, M. Valer, and T. Preckel, “Single-cell analysis of yeast, mammalian cells, and fungal spores with a microfluidic pressure-driven chip-based system”, Cytometry A 59, 246‒253 (2004).
  29.  M. Held, C. Edwards, and D.V. Nicolau, “Examining the behaviour of fungal cells in microconfined mazelike structures”, in Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 2008.
  30.  M. Held, O. Kašpar, C. Edwards, and D.V. Nicolau, “Intracellular mechanisms of fungal space searching in microenvironments”, PNAS 116, 13543‒13552 (2019).
  31.  E. Berthier, E.W. Young, and D. Beebe, “Engineers are from PDMS-land, Biologists are from Polystyrenia”, Lab Chip 12(7), 1224‒1237 (2012).
  32.  D. Stadnik, M. Chudy, Z. Brzózka, and A. Dybko, “Spectrophotometric analysis using poly (dimethylsiloxane) microfluidic detectors”, Bull. Pol. Ac.: Tech. 53(2), 163‒165 (2005).
  33.  X. Che, J. Boldrey, X. Zhong, S. Unnikandam-Veettil, I. Schneider, D. Jiles, and L. Que, “On-Chip Studies of Magnetic Stimulation Effect on Single Neural Cell Viability and Proliferation on Glass and Nanoporous Surfaces”, ACS Appl. Mater. Interfaces 10 (34), 28269‒28278 (2018).
  34.  C. Iliescu, F. Tay, and J. Miao, “Strategies in deep wet etching of Pyrex glass”, Sens. Actuator A Phys. 133, 395‒400 (2007).
  35.  R. Ma, Q. Chen, Y. Fan, Q. Wang, S. Chen, X. Liu, L. Cai, and B. Yao, “Six new soil–inhabiting Cladosporium species from plateaus in China”, Mycologia 109(2), 244‒260 (2017).
  36.  I. Ghiaie Asl, M. Motamedi, G.R. Shokuhi, N. Jalalizand, A. Farhang, and H. Mirhendi, “Molecular characterization of environmental Cladosporium species isolated from Iran.” Curr. Med. Mycol 3(1), 1–5 (2017).
  37.  T. Watanabe, Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species, CRC Press, Washington, USA (2011).
  38.  K.H. Domsch, W. Gams, and T.H. Anderson. Compendium of Soil Fungi, T: 1, Academic Press, London, UK, 1980.
  39.  J.C. Gilman, A manual of soil fungi, Ed. 2, Iowa State College Press, Ames, USA, 1957.
  40.  W. Pusz, K. Patejuk, and A. Kaczmarek, “Fungi colonizing of small balsam seeds (Impatiens parviflora DC.) seeds in Wigry National Park”, Prog. Plant Prot. 60, 33‒40 (2020).
  41.  B. Jacewski, J. Urbaniak, P. Kwiatkowski, and W. Pusz, “Microfungal diversity of Juncus trifidus L. and Salix herbacea L. at isolated locations in the Sudetes and Carpathian Mountains”, Acta Mycol. 54(1), 1118 (2019).
  42.  E. Levetin and K. Dorseys, “Contribution to leaf surface fungi to the air spora.” Aerobiologia 22, 3‒12 (2006).
  43.  S.N. Stohr and J. Dighton, “Effects of species diversity on establishment and coexistence: A phylloplane fungal community model system”, Microb. Ecol. 48, 431‒438 (2004).
  44.  E.M. El-Morsy, “Fungi isolated from the endorhizosphere of halophytic plants from the Red Sea Coast of Egypt”, Fungal Divers. 5, 43‒54 (2000).
  45.  K. Bensch, U. Braun, J.Z. Groenewald, and P.W. Crous, “The genus Cladosporium”, Stud. Mycol. 72, 1‒401 (2012).
  46.  M.B. Ellis, Dematiaceous Hyphomycetes, Commonwealth Mycological Institute, Kew, Surrey, UK, 1971.
  47.  R. Ogórek, A. Lejman, W. Pusz, A. Miłuch, and P. Miodyńska, “Characteristics and taxonomy of Cladosporium fungi”, Med. Mycol. J. 19(2), 80‒85 (2012).
  48.  S. Sharma, R.C. Sharma, and R. Malhotra, “Effect of the Saprophytic Fungi Alternaria alternata and Cladosporium oxysporum on Germination, Parasitism and Viability of Melampsora ciliata Urediniospores”, J. Plant. Dis. Prot. 109(3), 291‒300 (2002).
  49.  W. Pusz, R. Weber, A. Dancewicz, and W. Kita, “Analysis of selected fungi variation and its dependence on season and mountain range in southern Poland – key factors in drawing up trial guidelines for aeromycological monitoring”, Environ. Monit. Assess. 189(10), 526 (2017).
  50.  W. Pusz, W. Kita, A. Dancewicz, and R. Weber, “Airborne fungal spores of subalpine zone of the Karkonosze and Izerskie Mountains (Poland)”, J. Mt. Sci. 10(10), 940–952 (2013).
  51.  W. Pusz, M. Król, and T. Zwijacz-Kozica, “Airborne fungi as indicators of ecosystem disturbance: an example from selected Tatra Mountains caves (Poland)”, Aerobiologia 34, 111‒118 (2018).
  52.  E. Porca, V. Jurado, P.M. Martin-Sanchez, B. Hermosin, F. Bastian, C. Alabouvette, and C. Saiz-Jimenez, “Aerobiology: An ecological indicator for early detection and control of fungal outbreaks in caves”, Ecol. Indic. 11(6), 1594‒1598 (2011).
  53.  P. Gutarowska, “Moulds in biodeterioration of technical materials”, Folia Biologica et Oecologica 10, 27‒39 (2014).
  54.  B. Zyska and Z. Żakowska. Mikrobiologia materiałów, Politechnika Łódzka, Łódź, 2005.
  55.  T.J. Berryman. “Fuel Quality and demand – an overview” in Microbiology of fuels, Ed. R.N. Smith, Institute of Petroleum, London, UK, 1987.
  56.  K. Schubert, J.Z. Groenewald, U. Braun, J. Dijksterhuis, M. Starink, C.F. Hill, P. Zalar, G.S. de Hoog, and P.W. Crous, “Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics”, Stud. Mycol. 58, 105‒156 (2007).
  57.  J. Israel Martínez-López, M. Mojica, C.A. Rodríguez, and H.R. Siller, “Xurography as a Rapid Fabrication Alternative for Point-of-Care Devices: Assessment of Passive Micromixers”, Sensors (Basel) 16(5), 705 (2016).
  58.  D. Witkowski, W. Kubicki, J.A. Dziuban, D. Jašíková, and A. Karczemska, “Micro-particle image velocimetry for imaging flows in passive microfluidic mixers”, Bull. Pol. Ac.: Tech. 25(3), 441–450 (2018).
  59.  A. Lamberti, S.L. Marasso, and M. Cocuzza, “PDMS membranes with tunable gas permeability for microfluidic applications”, RSC Adv. 4, 61415–61419 (2014).
  60.  K. Kamei, Y. Mashimo, and Y. Koyama, “3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients”, Biomed. Microdev. 17(2), 36 (2015).
  61.  P. Thurgood, S. Baratchi, C. Szydzik, A. Mitchella, and K. Khoshmanesh, “Porous PDMS structures for the storage and release of aqueous solutions into fluidic environments”, Lab Chip 17, 2517‒2527 (2017).
  62.  A. Podwin, R. Walczak, and J.A. Dziuban, “A 3D printed membrane-based gas microflow regulator for on-chip cell culture”, Appl. Sci. 8(4), 579 (2018).
  63.  A. Podwin and J.A. Dziuban, “Modular 3D printed lab-on-a-chip bio-reactor for the biochemical energy cascade of microorganisms”, J. Micromech. Microeng. 27(10), 104004 (2017).
  64.  A. Podwin, W. Kubicki, K. Adamski, R. Walczak, and J.A. Dziuban, “A step towards on-chip biochemical energy cascade of microorganisms: Carbon dioxide generation induced by ethanol fermentation in 3D printed modular lab-on-a-chip”, J. Phys.: Conf. Ser. 773(1), 012052 (2016).
  65.  K. Ozasa, J. Lee, S. Song, M. Hara, and M. Maeda, “Gas/liquid sensing via chemotaxis of euglena cells confined in an isolated micro- aquarium”, Lab Chip 13, 4033‒4039 (2013).
  66.  F.J.H. Hol and C. Dekker, “Zooming in to see the bigger picture: Microfluidic and nanofabrication tools to study bacteria”, Science 346 (6208), 1251821 (2014).
  67.  K. Nagy, Á. Ábrahám, J.E. Keymer, and P. Galajda, “Application of Microfluidics in Experimental Ecology: The Importance of Being Spatial”, Front. Microbiol. 9, 496 (2018)
  68.  A. Podwin, W. Kubicki, and J.A. Dziuban, “Study of the behavior of Euglena viridis, Euglena gracilis and Lepadella patella cultured in all-glass microaquarium”, Biomed. Microdev. 19(3), 63 (2017).
  69.  R. Walczak, P. Śniadek, J.A. Dziuban, J. Kluger, and A. Chełmońska-Soyta, “Supravital fluorometric apoptosis detection in a single mouse embryo using lab-on-a-chip”, Lab Chip 11, 3263‒3268 (2011).
  70.  A. Podwin, D. Lizanets, D. Przystupski, W. Kubicki, P. Śniadek, J. Kulbacka, A. Wymysłowski, R. Walczak, and J.A. Dziuban, “Lab-on- Chip Platform for Culturing and Dynamic Evaluation of Cells Development”, Micromachines 11(2), 196 (2020).
  71.  W. Wei, et al., “A numerical model for air concentration distribution in self-aerated open channel flows”, J. Hydrodynam. B. 27(3), 394‒402 (2015).
  72.  S. Agaoglu, et al., “The effect of pre-polymer/cross-linker storage on the elasticity and reliability of PDMS microfluidic devices”, Microfluid. Nanofluidics 21, 117 (2017).
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Authors and Affiliations

Agnieszka Podwin
1
Tymon Janisz
1
Katarzyna Patejuk
2
Piotr Szyszka
1
Rafał Walczak
1
Jan Dziuban
1
  1. Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, ul. Janiszewskiego 11/17, 50-372 Wrocław, Poland
  2. Wrocław University of Environmental and Life Sciences, Department of Plant Protection, Grunwaldzki Sq. 24a, 50-363 Wroclaw, Poland

Study on the Effect of in Situ Surface Treatments of Medical Microfluidic Systems Obtained Through Additive Manufacturing

A. Csapai D.-A. Țoc V. Pașcalău V. Toșa D. Opruța F. Popa C. Popa
Archives of Metallurgy and Materials | 2022 | vol. 67 | No 3 | 1071-1078 | DOI: 10.24425/amm.2022.139705
Keywords additive manufacturing microfluidic systems surface roughness biofilm growth laminar flow
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Abstract

This paper presents a comparative study on the effects of the in-situ surface modifications performed on “H” type microfluidic systems obtained via additive manufacturing. The microsystem was printed using a polylactic acid filament on an Ender-5 Pro printer. The surface modification of the main channel was done using chloroform by two different methods: vapor smoothing and flushing. The obtained surface roughness was studied using an optical microscope and the ImageJ software, as well as scanning electron microscopy. The effect of the channel surface treatment upon the characteristics of the fluid flow was assessed. The microfluidic systems were used for the dynamic study of biofilm growth of Candida albicans (ATCC 10231). The influence of the surface roughness of the main channel on the formation and growth of the biofilm was studied using quantitative methods, scanning electron microscopy imaging as well as optical coherence tomography.
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Authors and Affiliations

A. Csapai
1
ORCID: ORCID
D.-A. Țoc
2
ORCID: ORCID
V. Pașcalău
1
ORCID: ORCID
V. Toșa
1
ORCID: ORCID
D. Opruța
3
ORCID: ORCID
F. Popa
1
ORCID: ORCID
C. Popa
ORCID: ORCID
  1. Materials Science and Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania
  2. Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400000 Cluj-Napoca, Romania
  3. Thermal Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania

Micro-Particle Image Velocimetry for imaging flows in passive microfluidic mixers

Dariusz Witkowski Wojciech Kubicki Jan A. Dziuban Darina Jašíková Anna Karczemska
Metrology and Measurement Systems | 2018 | vol. 25 | No 3 | 441–450 | DOI: 10.24425/123890
Keywords micro-Particle Image Velocimetry microfluidics microfluidic devices
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Abstract

The micro-Particle Image Velocimetry (micro-PIV) was used to measure flow velocities in micro-channels

in two passive micromixers: a microfluidic Venturi mixer and a microfluidic spiral mixer, both preceded

by standard “Y” micromixers. The micro-devices were made of borosilicate glass, with micro-engineering

techniques dedicated to micro-PIV measurements. The obtained velocity profiles show differences in the

flow structure in both cases. The micro-PIV enables understanding the micro-flow phenomena and can help

to increase reproducibility of micromixers in mass production.

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Authors and Affiliations

Dariusz Witkowski
Wojciech Kubicki
Jan A. Dziuban
Darina Jašíková
Anna Karczemska

Spectrophotometric analysis using poly(dimethylsiloxane) microfluidic detectors

D. Stadnik M. Chudy Z. Brzózka A. Dybko
Bulletin of the Polish Academy of Sciences Technical Sciences | 2005 | vol. 53 | No 2 | 163-165
Keywords poly(dimethylsiloxane) polymer optical fibres microfluidic structures
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Abstract

Two constructions of microfluidic structures are described in this paper. A fibre optic microcell for spectrophometric measurements and a microcell for fluorescence experiments were designed and tested. The structures were made of polymer optical fibres which were incorporated into polymeric material i.e. poly(dimethylsiloxane). The structures were tested as detectors in absorbance measurement (solutions of bromothymol blue with diffierent pH were used) and in fluorescence tests (solution of fluoresceine was used).

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Authors and Affiliations

D. Stadnik
M. Chudy
Z. Brzózka
A. Dybko

Technology and applications of Low Temperature Cofired Ceramic (LTCC) based sensors and microsystems

L.J. Golonka
Bulletin of the Polish Academy of Sciences Technical Sciences | 2006 | vol. 54 | No 2 | 221-231
Keywords thick film LTCC sensor microsystem microfluidic
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Abstract

The paper presents general information on LTCC materials, manufacturing processes and properties of fired modules. A Multichip Module package has been the main application of Low Temperature Cofired Ceramic (LTCC) technology. Recently, this technology is also used for production of sensors, actuators and microsystems. The research and development on the LTCC sensors and microsystems carried out in the Laboratory of Thick Film Microsystems at Wroclaw University of Technology are presented. LTCC microfluidic system is described in detail. Moreover, a short information is given on other LTCC applications .

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Authors and Affiliations

L.J. Golonka

Lab-on-a-Chip Microdevice with Contactless Conductivity Detector

Karolina Blaszczyk Michal Chudy Zbigniew Brzózka Artur Dybko
Metrology and Measurement Systems | 2013 | No 2 | 299-306 | DOI: 10.2478/mms-2013-0026
Keywords lab-on-a-chip poly(dimethylsiloxane) contactless conductivity pseudoelectrodes microfluidic
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Abstract

This paper describes a new contactless conductivity detector, whose electrodes are constructed of microchannels filled with solution of KCl - called pseudoelectrodes. The lab-on-a-chip microdevice was fabricated in poly(dimethylsiloxane) PDMS, using a moulding technique. The mould was made from a dry negative photoresist with a thickness of 50 μm. During the tests, the dimension! and arrangement of pseudoelectrodes` microchannels were evaluated. The analyte was pumped into the microchannel using a syringe pump with a flow rate of 50 μL/min. Reproducible!changes of the signal were obtained.

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Authors and Affiliations

Karolina Blaszczyk
Michal Chudy
Zbigniew Brzózka
Artur Dybko

Formation of bubbles and droplets in microfluidic systems

P. Garstecki A.M. Gańán-Calvo G.M. Whitesides
Bulletin of the Polish Academy of Sciences Technical Sciences | 2005 | vol. 53 | No 4 | 361-372
Keywords formation of bubbles and droplets microfluidic systems hydrodynamic techniques
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Abstract

This mini-review reports the recent advances in the hydrodynamic techniques for formation of bubbles of gas in liquid in microfluidic systems. Systems comprising ducts that have widths of the order of 100 micrometers produce suspensions of bubbles with narrow size distributions. Certain of these systems have the ability to tune the volume fraction of the gaseous phase – over the whole range from zero to one. The rate of flow of the liquids through the devices determines the mechanism of formation of the bubbles – from break-up controlled by the rate of flow of the liquid (at low capillary numbers, and in the presence of strong confinement by the walls of the microchannels), to dynamics dominated by inertial effects (at high Weber numbers). The region of transition between these two regimes exhibits nonlinear behaviours, with period doubling cascades and irregular bubbling as prominent examples. Microfluidic systems provide new and uniquely controlled methods for generation of bubbles, and offer potential applications in micro-flow chemical processing, synthesis of materials, and fluidic optics.

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Authors and Affiliations

P. Garstecki
A.M. Gańán-Calvo
G.M. Whitesides

X-ray microdiffraction on flow-controlled biomolecular assemblies

H.M. Evans R. Dootz S. Köster B. Struth T. Pfohl
Bulletin of the Polish Academy of Sciences Technical Sciences | 2007 | vol. 55 | No 2 | 217-227
Keywords X-ray diffraction hydrodynamic focusing DNA microfluidic self-assembly lipoplex
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Abstract

The study of liquid crystalline assemblies, with an emphasis on biological phenomena, is now accessible using newly developed microdevices integrated with X-ray analysis capability. Many biological systems can be described in terms of gradients, mixing, and confinement, all of which can be mimicked with the use of appropriate microfluidic designs. The use of hydrodynamic focusing creates well-defined mixing conditions that vary depending on parameters such as device geometry, and can be quantified with finite element modelling.We describe experiments in which geometry and strain rate induce finite changes in liquid crystalline orientation. We also demonstrate the online supramolecular assembly of lipoplexes. The measurement of lipoplex orientation as a function of flow velocity allows us to record a relaxation process of the lipoplexes, as evidenced by a remarkable 4-fold azimuthal symmetry. All of these processes are accessible due to the intentional integration of design elements in the microdevices.

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Authors and Affiliations

H.M. Evans
R. Dootz
S. Köster
B. Struth
T. Pfohl

Integration of Optoelectronic Components with LTCC (Low Temperature Co-Fired Ceramic) Microfluidic Structure

Karol Malecha
Metrology and Measurement Systems | 2011 | No 4 | 713-722 | DOI: 10.2478/v10178-011-0067-3
Keywords LTCC (low temperature co-fired ceramics) microfluidic absorbance CFD (computational fluid dynamics)
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Abstract

Investigations on integration of optoelectronic components with LTCC (low temperature co-fired ceramics) microfluidic module are presented. Design, fabrication and characterization of the ceramic structure for optical absorbance is described as well. The geometry of the microfluidic channels has been designed according to results of the CFD (computational fluid dynamics) analysis. A fabricated LTCC-based microfluidic module consists of an U-shaped microchannel, two optical fibers and integrated light source (light emitting diode) and photodetector (light-to-voltage converter). Properties of the fabricated microfluidic system have been investigated experimentally. Several concentrations of potassium permanganate (KMnO4) in water were used for absorbance/transmittance measurements. The test has shown a linear detection range for various concentrations of heavy metal ions in distilled water. The fabricated microfluidic structure is found to be a very useful system in chemical analysis.

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Authors and Affiliations

Karol Malecha

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