The research on impact of Cleonus piger Scop. (Coleoptera; Curculionidae) feeding and the occurrence of other pest insects on milk thistle plants grown in monoculture and crop rotation after cereals, with two different seeding dates was carried out in the years 2003–2005. The infestation and density of C. piger larvae in roots of plants grown in monoculture increased with subsequent developmental phases and subsequent years of the experiment. Feeding resulted in the decrease in crop yield by 40% compared to the crop rotation treatment. In crop rotation stands, the infestation of milk thistle roots by C. piger larvae was 4–5 times lower at the final phase than in monoculture. Postponing seeding by three weeks led to the decrease of infestation and density of C. piger larvae, but the crop yield was lower than that from the earlyseeded stands. No other phytophagous species of economic importance were found.

The species structure of plant parasitic nematode populations from the rhizosphere of winter wheat grown with crop rotation or in 48-year-old monoculture was analyzed and compared. Dominating species: Bitylenchus dubius, Merlinius microdorus, Paratylenchus neglectus and Heterodera avenae, in monoculture plots, had higher populations than in crop rotation plots. Heterodera avenae eggs and larvae were infected by pathogenic fungi in 68% of the monoculture crops (vs. 65–66% of the cysts from crop rotation), 12–20% of Paratylenchus sp. specimens were colonized by bacteria, mainly by Bacillus penetrans. This study shows nematological changes occurring in long-term wheat breeding, thus providing additional information necessary to fight dangerous viral vectors of the examined cereal.

The aim of performed research was to evaluate weed seedbank in soil under the influence of four different winter wheat tillage systems. Winter wheat was grown in the following cultivation systems: A – monoculture with direct drilling into white clover mulch; B – monoculture with direct drilling into wheat stubble; C – monoculture with conventional tillage; D – crop rotation with conventional tillage. It was shown that pre-sowing wheat tillage had a more considerable effect on weed species and weed seedbank in soil than type of crop rotation. The least seedbank was observed when plough system was replaced by direct drilling. In the soil layer of 0–20 cm, under wheat no-plough tillage, 20.3% less weed diaspores wasfound compared to monoculture with plough tillage and by 40.1% lessthan in crop rotation. The plough tillage increased amount of weed diaspores in the whole plough layer, while direct drilling increased it only in 0–1 cm of soil layer. After direct drilling of wheat into stubble (B) the number of weed diaspores in 1 dcm³ of soil in 0–1 cm layer was over twofold higher than in direct sowing in mulch (A), and threefold higher than in crop rotation (D) and almost six times higher than in wheat monoculture with conventional tillage (C). Dominating weed species in the soil over the types of wheat cultivation systems were: Chenopodium album L., Amaranthus retroflexus L., Apera spica-venti L., Lamium purpureum L., and Viola arvensis Murr.