The stress superposition effect in the midline area of the multi-wheeled vehicles produced by moving vehicles load is generally ignored, which leads to smaller results in research of subgrade service depth. Based on the elastic mechanics theory, the analytical solution of subgrade dynamic response under moving vehicles load is derived with compound elastic layers. The characteristics of subgrade dynamic stress distribution under the action of moving vehicles are analyzed by using Midas Gts Nx numerical simulation software, and the influence of static and dynamic axle load on the subgrade service depth is compared. The results demonstrate that the subgrade dynamic stress in the under-wheel area attenuates rapidly along the depth direction, while the subgrade dynamic stress in the midline area increases at first and subsequently decreases along the same direction. With the increase of subgrade dynamic stress, the shape of dynamic stress isosurface changes from bimodal to unimodal. Whether in the form of static or dynamic axle load, the subgrade service depth in the middle line area is larger than that in under-wheel area, and the influence of dynamic axle load on the subgrade service depth is greater than that of static axle load. The wheel distance and vehicle velocity have a significant influence on the subgrade service depth.With the increase of vehicle velocity, the subgrade service depth decreases. With the increase of wheel distance, the subgrade service depth decreases.