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Analysis of peripheral blood chemiluminescence in horses

W. Krumrych J. Danek H. Markiewicz M. Gołyński

Polish Journal of Veterinary Sciences | 2021 | vol. 24 | No 4 | 537-546 | DOI: 10.24425/pjvs.2021.139978

Keywords horses blood neutrophils chemiluminescence

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Abstract

The aim of this study was to analyze the chemiluminescence (CL) of peripheral blood in clinically healthy horses of different sexes and ages. The tests were carried out on 119 half- -breed horses, representing various forms of use (66 recreational horses and 53 sport horses). The test material was peripheral blood, which was collected under resting conditions, i.e. before physical activity related to the use of these animals. In the blood samples, spontaneous and stimulated CL with zymosan and phorbol myristate acetate were determined. It has been found that regular training effort increases the blood’s pro-oxidative potential, which was demonstrated by significantly higher (p<0.05) CL values in sport horses compared to recreational animals. Analysis of the results did not show any statistically significant correlation between sex or age of the horses with chemiluminescence values in peripheral blood. The result of the research suggests the need to optimize the results of blood CL measurements, taking into account the number of neutrophils and the concentration of hemoglobin in the blood of tested animals. Analysis of non-optimized blood CL results may lead to premature conclusions.

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Authors and Affiliations

W. Krumrych

J. Danek

H. Markiewicz

M. Gołyński

Department of Microbiology and Immunobiology, Kazimierz Wielki University, Powstańców Wlkp. Avenue 10, 85-090, Bydgoszcz, Poland
Department of Biomedical Engineering, UTP University of Science and Technology, prof. S. Kaliskiego Avenue 7, 85-796 Bydgoszcz, Poland
Department of Animal Breeding, Faculty of Animal Breeding and Biology, UTP University of Science and Technology, Mazowiecka Street 28, 85-082 Bydgoszcz, Poland
Department of Diagnostics and Clinical Sciences, Veterinary Medicine Institute, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska Street 1, 87-100 Toruń, Poland

Extrathoracic negative pressure ameliorates lung injury during mechanical ventilation in experimental pigs

J. Wessely-Szponder T. Szponder A. Fijalkowska-Nestorowicz R. Bobowiec A. Sobczyńska

Polish Journal of Veterinary Sciences | 2018 | vol. 21 | No 4 | 697-703 | DOI: 10.24425/124308

Keywords neutrophil mechanical ventilation lung injury extrathoracic negative pressure

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Abstract

Mechanical ventilation (MV) is a supportive and life-saving therapy, however, it can cause ventilator-induced lung injury as a common complication. Thus, recruitment manoeuvres (RM) are applied to open the collapsed alveoli to ensure sufficient alveolar surface area for gas exchange. In the light of the fact that positive pressure ventilation is currently the standard treat- ment for improving pulmonary function, extrathoracic negative pressure is considered as an alter- native form of respiratory support. The aim of this study was to estimate the proinflammatory and oxidative response during MV and lung injury as well as the response after RM. All studied parameters were assessed at the following time points: T1-spontaneous breathing, T2- MV, T3- lung injury, T4 –RM. During MV (T2) elastase, MPO, ALP release, nitrite and superoxide generation significantly increased, whereas in later measurements a decrease in these values was noted. The MDA plasma concentration significantly (p<0.05) increased at T2, reaching a level of 13.30±0.87 nmol/ml; at other time points the values obtained were similar to the baseline value of 9.94±0.94 nmol/ml, whereas a gradual decrease in SOD activity at time T2-T4 points in comparison with the baseline value was found. During the study both neutrophil activity and oxi- dative stress indicate exacerbated response after MV and lung injury by bronchoalveolar lavage; however, extrathoracic negative pressure system as the MR ameliorates damaging changes which could further lead to serious lung injury.

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Authors and Affiliations

J. Wessely-Szponder

T. Szponder

A. Fijalkowska-Nestorowicz

R. Bobowiec

A. Sobczyńska

Neutrophil-to-lymphocyte ratio predicts long-term all-cause mortality in patients with chronic kidney disease stage 5

Karolina Woziwodzka Artur Dziewierz Magdalena Pawica Agnieszka Panek Marcin Krzanowski Paulina Gołasa Paweł Latacz Magdalena Burkat Marek Kuźniewski Katarzyna Krzanowska

Folia Medica Cracoviensia | 2019 | vol. 59 | No 4 | 55-70 | DOI: 10.24425/fmc.2019.131380

Keywords neutrophil-to-lymphocyte ratio CV mortality all-cause mortality CKD stage

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Abstract

I n t r o d u c t i o n: A high neutrophil-to-lymphocyte ratio (NLR) has been reported to be a strong biomarker of inflammation.

A i m: We sought to evaluate the impact of NLR on long-term all-cause and cardio-vascular (CV) mortality in hemodialysis (HD) patients.

Ma t e r i a l a n d Me t h o d s: A total of 84 chronic kidney disease (CKD) stage 5 patients with 54 of them on HD, with a median age of 61.5 (51.3–74.8) years were enrolled. The association between NLR and clinical biomarkers was investigated. Multivariable Cox regression analysis was used to find significant predictors of all-cause and CV mortality at follow-up.

R e s u l t s: The median NLR (interquartile range) was 3.0 (2.1–4.1). Patients with NLR ≥3.9 (the highest tertile) had higher five-year all-cause mortality then remaining patients (53.6% vs. 30.4%; p = 0.039). On the contrary, only a trend towards increased CV mortality was observed (25.0% vs. 42.9%; p = 0.10). NLR ≥3.9 was a significant predictor of all-cause mortality at five years [hazard ratio (95%CI): 2.23 (1.10– 4.50); p = 0.025] in Cox regression model adjusted for age, gender, and diabetes status. Similarly, while using NLR as continuous variable a significant association between NLR and all-cause mortality was confirmed even after adjustment for covariates [hazard ratio per 1 unit increase (95%CI): 1.26 (1.06–1.51); p = 0.009] with the area under the receiver operating characteristic (ROC) curve of 0.64. Correlations between NLR and WBC, concentration of fibrinogen, albumin were observed.

C o n c l u s i o n s: Asymptomatic inflammation measured by NLR showed an association with long-term all-cause mortality in stage 5 CKD patients, even while white blood cell count was in the normal range.

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Authors and Affiliations

Karolina Woziwodzka

Artur Dziewierz

Magdalena Pawica

Agnieszka Panek

Marcin Krzanowski

Paulina Gołasa

Paweł Latacz

Magdalena Burkat

Marek Kuźniewski

Katarzyna Krzanowska

The applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats on the example of animals supplemented with β-hydroxy-β-methylbutyrate (HMB)

R. Wójcik J. Małaczewska E. Kaczorek-Łukowska

Polish Journal of Veterinary Sciences | 2019 | vol.22 | No 4 | 725-733 | DOI: 10.24425/pjvs.2019.131402

Keywords goats Migratest® kit chemotactic activity of peripheral blood neutrophils β-hydroxy-β-methylbutyrate

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Abstract

The objective of this study was to determine the applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats. The experiment was performed on 14 goat kids aged 30±2 days, divided into two groups of 7 animals each: C - control group, and E – experimental group, supplemented with β-hydroxy-β-methylbutyrate (HMB), a typical immunostimulant which influences the phagocytic activity of peripheral neutrophils. The feed administered to experimental goat kids was supplemented with HMB at 40 mg/kg BW, whereas control goat kids were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the chemotactic activity of peripheral blood neutrophils in goats. The results of the study indicate that the Migratest® kit can be used to evaluate the influence of immunomodulators on the chemotactic activity of peripheral blood neutrophils in goats. The results of the assay are most effectively presented by calculating the chemotactic index which accounts for the chemotaxis or migration of neutrophils in the presence or absence of a chemotactic factor, respectively, and the percentage of granulocytes that migrate towards fMLP. The results of both presentation methods appear to be identical.

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Authors and Affiliations

R. Wójcik

J. Małaczewska

E. Kaczorek-Łukowska

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Analysis of peripheral blood chemiluminescence in horses

Abstract

Bibliography

Authors and Affiliations

Extrathoracic negative pressure ameliorates lung injury during mechanical ventilation in experimental pigs

Abstract

Authors and Affiliations

Neutrophil-to-lymphocyte ratio predicts long-term all-cause mortality in patients with chronic kidney disease stage 5

Abstract

Authors and Affiliations

The applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats on the example of animals supplemented with β-hydroxy-β-methylbutyrate (HMB)

Abstract

Authors and Affiliations