The text presents the achievements of Wiesław Juszczak, who died in 2021, as a challenge that has not yet been taken up by the humanities. The reason for this may be the multiformity and thematic richness of his texts, almost always essayistic in nature (in the best, traditional sense), with an outstanding literary dimension, but at the same time a deep and broad scientific background. This applies to both his books on Polish painting around 1900, works on post-impressionists, as well as analyzes of archaic Greek thought about art, religious roots of art from various cultures, unconventional interpretations of Japanese films or Ingmar Bergman’s oeuvre.

This paper presents the results of observations on occurrence of Tetranychidae. During 1995-2001 investigations a total of 10 species, namely Bryobia praetiosa, Eotetranychus carpini, E. cary/i, E. pruni, E. tiliarum, Eurytetranychus buxi, Panonychus ulmi, Schizotetranychus schizopus, Tetranychus viennensis and T. urticae were collected in ornamental nurseries in Poland. The T. urticae was the most frequent species ofmites and it was found on 60 different species of plants belonging to 1 7 families. This species caused severe damage on Spiraea japonica (Rosaceae), Ulmus minor 'Jacqueline Hillier' (Ulmaceae), Magnolia spp. (Magnoliaceae), Buddleja davidii (Buddlejaceae), Sambucus nigra (Caprifoliaceae), Laburnum xwatererii (Fabaceae) and Daphne mezereum (Thymelaceae). Among ocher tetranychids, S. schizopus is also dangerous pest of grafted willows.

RNA extraction involves several main stages, regardless of the method of extraction: homogenization, effective denaturation of proteins from RNA, inactivation of ribonuclease and removal of any DNA, protein, and some residual contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of polysaccharides and phenols. Several efforts have been made towards the comparison and optimization of extraction and purification methods for RNA from plant tissues. This is dictated by the necessity of obtaining RNA of a good quality and in a sufficient quantity for further molecular analyzes. Plant storage organs (such as bulbs or seeds) rich in polysaccharide and polyphenolic compounds present distinct challenges for total RNA isolation. Such components, considered in this case as contamination, may bind and co-precipitate with nucleic acids and negatively affect later assays. Since standard routine protocols yield unacceptable results in bulbs, we have designed a new method for RNA extraction. We used two modified procedures (based on CTAB and sarkosyl reagents) of RNA extraction from so called “difficult plant material” and compared them to a popular RNA isolation base on the column isolation kit and TriPure reagent. Our modified protocols dealt with problems of both RNA degradation and low yield caused by co-purification with polysaccharides present in plant bulbs. In this study we have shown that improvement of the CTAB and sarkosyl method with a lyophilization step of plant tissues leads to isolation of high quality RNA from difficult material like storage organs of bulbous plants. The main changes in the procedure compared to the previously described methods concerned the different order of lithium chloride and sodium acetate addition, lithium chloride concentration increase and modification of centrifugation conditions. Gel electrophoresis and spectrophotometer analysis confirmed the high quality and integrity of the obtained RNA. The modified procedures allowed for obtaining a satisfying amount of RNA concentration in the range from 280 to 950 ng/μl depending on the plant species. Thus, the demonstrated RNA isolation methods are efficient and can be used for plant material rich in polysaccharides, such as bulbs.

Chrysanthemum stunt viroid (CSVd) is a serious pathogen infecting chrysanthemum worldwide. To improve and enhance the detection procedure, a colorimetric loop-mediated isothermal amplification (LAMP) technique was developed. Six LAMP primers were newly designed and tested to determine the optimal conditions using a recombinant plasmid of CSVd as a DNA template. The optimal conditions for colorimetric LAMP were incubation at 65°C for 45 min. Under these conditions, a ladder-like pattern of LAMP products was detected along with a change of color from pink to yellow in the positive reactions. Limits of the detection (LOD) of colorimetric LAMP were up to 1 fg ∙ μl–1 of plasmid DNA concentration which was 104 times greater than that of polymerase chain reaction (PCR). The developed colorimetric LAMP was not cross reacted to other viruses and viroids. From detection of actual samples and chrysanthemum plantlets which were obtained from meristem tip culture, the colorimetric LAMP showed effective potential in detecting CSVd. Therefore, the colorimetric LAMP can be used as a main technique to detect CSVd and ensure CSVd-free chrysanthemum plantlet production due to its accuracy, rapidness and sensitivity.

The subject of this article is the fragmentary silver plate of a gilded silver sheet braid ornament decorated with palmette motifs, which was deposited in the storage of the Gorgippia Archaeological Museum (Krasnodar Krai, Russia) in 2015, together with several other finds. The finds had been discovered at a site named Andreyevskaya Shhel, located a few kilometres south-east of the town, at the north-western hill area of the Caucasus. Among the artefacts deposited in the storage in 2015, there were other finds related to the 9–10th centuries (e.g. silver plate of a sabretache, gilded bronze belt mounts, bronze strap end, sabre, bow case or sabretache mount, fingering, etc). The braid ornament, with many analogies in the Carpathian Basin, could have reached the North Caucasian region by means of long-distance trade. This hypothesis is sustained by the considerable dirham-finds in the Carpathian Basin, which indicate the integration of this region – and of early Hungarian commerce as a whole – into the Eastern, Muslim trade network.