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Abstract

Since silver nanoparticles (AgNPs) are used as nanofungicides and nanopesticides in agriculture, the toxicity of AgNPs as well as AgNO3 must be determined. Besides this, we evaluated the combined effects of salicylic acid (SA) and nitric oxide (NO) on responses of Phlomis tuberosa plants to Ag-induced stress. The results of growth parameters together with measurement of malondialdehyde (MDA) indicated that exposure to 1000 mg L–1 of AgNPs or AgNO3 exerted more toxicity, which was closely associated with the over– accumulation of ROS and the reduction of photochemical functioning. However, SNP (NO) and SA addition successfully alleviated adverse impact of AgNPs on Phlomis seedlings. Maximum amelioration of Ag-induced stress was found by combined treatments of SA+NO. Phlomis plants primed with SA+NO exhibited higher synthesis of chlorophyll b and carotenoid pigments to ameliorate AgNP-induced adverse effects on chlorophyll fluorescence parameters. SA+NO led to high levels of proline under both AgNPs and AgNO3 treatments. A further increase in antioxidants (phenolic compounds) was observed in NO-primed plants under AgNPs- induced stress, which was attendant with the high level of CAT and APX activities. Increase in total Ag translocation into shoot organs and cell survival were also enhanced by SA+NO under AgNPs stress. We concluded that SA+NO mitigated the inhibitory effects of AgNPs stress on the photosynthetic apparatus by increasing the phenolic compounds and carotenoids as well as by regulating accumulation of Ag, ROS and antioxidants. The present findings provide important knowledge to design strategies that minimize the negative impact of AgNPs and AgNO3 on crops.
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Authors and Affiliations

Elham Ghasemifar
1
Ghader Habibi
1
Golamreza Bakhshi-Khaniki
1

  1. Department of Biology, Payame Noor University (PNU), PO BOX 19395-3697 Tehran, Iran
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Abstract

When exposed to high cadmium concentrations applied to the soil, the abiotic stress-tolerant, semi-halophytic C3/CAM (Crassulacean Acid Metabolism) photosynthetic intermediate plant Mesembryanthemum crystallinum L. demonstrates negligible poisoning symptoms with well-protected photochemical activity. Gas exchange analysis of the soil-grown plants exposed to Cd concentrations ranging from 0.01 to 10.0 mM revealed stimulation of net photosynthesis in the C 3 metabolic state, and this observation coincided with an increase in the transpiration level. The obtained results suggest that the initial action of Cd after the administration of this heavy metal is the stimulation of stomata opening.
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Authors and Affiliations

Adriana Maria Kaczmarczyk
1
ORCID: ORCID
Michał Nosek
2
Paweł Kaszycki
3
ORCID: ORCID
Paulina Supel
3
ORCID: ORCID
Zbigniew Miszalski
1

  1. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków, Poland
  2. Institute of Biology, University of the National Education Comission Kraków, Poland
  3. Department of Plant Biology and Biotechnology, University of Agriculture in Kraków

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