This paper presents the results of palaeobotanical studies (pollen and plant macrofossil analyses) of the sediments from the profile Michałowo P-3 (Gródek-Michałowo Depression, NE Poland). At this profile the second bed of peat under Holocene peat and the layer of silts and sands was discovered. The pollen succession indicates that they were deposited, with some gaps, from the beginning of Eernian Interglacial to Early Vistulian. The local pollen zones, distinguished in the pollen diagram, correlate with the regional pollen stratigraphy of the Eemian Interglacial, but the pollen record of hornbeam (ES R PAZ) and spruce (E6 R PAZ) regional zones is absent in Michałowo. In the middle part of the Michałowo pine zone (Mi-5 Pinus L PAZ), a cool oscillation of climate is marked by an opening of forests and development of herb plant communities.