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Abstract

In vitro test of antagonistic activity of culture filtrates from Trichoderma harzianum Rifai and Trichoderma pseudo-koningii Rifai strains against post-harvest pathogens of some fruits were investigated. The undiluted culture filtrates of the two Trichoderma species completely inhibited germination of conidia/spores of all the rot pathogens, but 50% dilution showed varying degree of inhibition of spore germination. T. pseudo-koningii culture filtrate had a rather moderate to strong inhibitory effect on mycelia of the pathogenic fungi. The highest per cent inhibition of 45.6% of mycelial growth was recorded for Aspergillus niger Tiegh.

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Authors and Affiliations

Chris Adegboyega Odebode
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Abstract

Kiwifruit (Actinidia deliciosa) is one of the most significant commercial crops in Iran. In 2015 a destructive disease of kiwifruits was observed in orchards, storage facilities and retail markets, resulting in great economic loss to producers. In this study phenotypic and molecular techniques were applied to characterize the causal agent of kiwifruit rot observed in Mazandaran province, northern Iran. From the similarity among the results of pathogenicity tests, equivalency with standard taxonomic criteria for disease and PCR-based analysis of the ITS region, all the isolates were identified as Botryosphaeria dothidea.

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Authors and Affiliations

Eisa Nazerian
Mansoureh Mirabolfathy
Samira Peighami Ashnaei
Farid Beiki
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Abstract

The main pathogen that deteriorates the quality of rose flowers during the postharvest stage belongs to the fungal genus Botrytis. The chemical products used to control the disease caused by this pathogen have been losing effectiveness due to the appearance of resistance. The present study describes the in vitro and in vivo fungicidal activity of Pelargonium graveolens essential oil and its chemical composition. The essential oil was obtained by hydrodistillation, and the in vitro fungicidal activity was determined by agar diffusion assays, showing 100% of fungal growth inhibition at 250 ppm. In vivo assays were performed on Rosa grandiflora flowers treated with 250 ppm of P. graveolens essential oil, using distillate water as a positive control and the commercial fungicide carbendazim as a negative one. No significant differences were obtained between the treatment with the essential oil and the treatment with the commercial fungicide. The chemical profile of the essential oil was determined by GC-MS. The main compounds detected were geraniol (24.89%), citronellol (19.50%), linalool (10.92%) and γ-eudesmol (8.93%). These results encourage the possible use of P. graveolens essential oil for the control of B. cinerea in rose flowers.
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Authors and Affiliations

María Inés Stegmayer
1
ORCID: ORCID
Norma Hortensia Álvarez
1 2
ORCID: ORCID
Néstor Gaspar Sager
2
ORCID: ORCID
Marcela Alejandra Buyatti
2
ORCID: ORCID
Marcos Gabriel Derita
1 3
ORCID: ORCID

  1. Producción y Protección Vegetal, ICiAgro Litoral, UNL, CONICET, FCA, Argentina
  2. Cultivos Intensivos, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Santa Fe, Argentina
  3. Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina

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