An application of biostimulants is becoming an increasingly popular operation in agriculture because they positively affect crop performance and qualitative characteristics, and prevent stress-related losses. The objective of this study was to determine the effect of an application of the following biostimulants: PlonoStart, Aminoplant, and Agro-Sorb Folium, and the herbicide Avatar 293 ZC on total carotenoids in table potato tubers. The research material consisted of tubers of two table potato cultivars, Oberon and Malaga, which were produced in a 3-year field experiment. Chemical analyses of fresh material were conducted 4–6 days following harvest. Biostimulants applied with the herbicide significantly increased the table potato tuber content of carotenoids compared to tubers obtained from unamended control plots. The highest accumulation of carotenoids was determined following an application of the biostimulant Agro-Sorb Folium, and it was higher in the tubers of cv. Oberon versus cv. Malaga. It should be added that in the available literature there is no research on the effect of biostimulants on the content of carotenoids in potato, which is the main food in the world. Carotenoids perform many important functions in the human body. They exhibit antioxidant properties, which means that they protect cells from damage, and also have a beneficial effect on the immune system. Carotenoids are substances that show an indispensable effect on the health and appearance of the skin. Regular consumption of them in the form of vegetables and fruits ensures its firmness, elasticity, smoothness, as well as a healthy appearance. The protective function of carotenoids against free radicals simultaneously contributes to slowing down the aging process. This action, in turn, translates into preventing the development of cancer or diseases of the circulatory system.