Ectopic pregnancy constitutes 2% of all pregnancies. In the last decades, due to the rising amount of caesarean sections, new localization of ectopic pregnancy has been observed — caesarean scar pregnancy (CSP). Cesarean scar pregnancy is an iatrogenic disease and a life-threatening condition which frequency will systematically rise. Because of possible serious complications, CSP should be swift ly diagnosed and treated. Th e purpose of this retrospective study was to demonstrate diff erent methods of CSP treatment performed between 2015–2018 in the Clinic of Endocrinological Gynaecology and Gynaecology Jagiellonian University Medical College in Cracow. Th e clinical characteristics, diagnosis, various methods of treatment and clinical outcomes were analysed. Defi nitive algorithm of CSP treatment is still not established. Pharmacological and operative methods are approved while expectant observation is considered unsafe due to possible risk of complications for the patient, including death.
One of the effective reproductive management programs in dairy cattle is the accurate detection of pregnancy. A total of 204 non-descriptive cows were examined for pregnancy before slaughter in Sulaimani abattoir. Examinations were done by rectal palpation and enzyme-linked immunosorbent assay (ELISA) to measure the levels of progesterone and bovine pregnancy-associated glycoproteins (bPAGs) in their blood. Detection of a live conceptus in the uterus of slaughtered cows was used as the gold standard to determine the accuracy of the three pregnancy detection methods. The results showed that the accuracies of rectal palpation, progesterone assay, and bPAGs assay in the diagnosis of pregnancy were 87.2%, 84.8%, and 97.05%, respectively. The bPAGs assay scored the highest sensitivity (100%) for detection of pregnancy, followed by the progesterone assay (92.3%) and rectal palpation (84.6%). In addition, the specificity of the bPAGs assay was the highest (96.0%), while progesterone assay exhibited the lowest specificity (80.1%) and rectal palpation showed a specificity rate of (88.8%). In conclusion, the best method for the detection of either for early or late pregnancy in cows was the bPAGs assay, which gave the lowest number of false-positive and false-negative results.
This study analysed the influence of montelukast (MON; 10-8 - 10-4 M), a cysteinyl leukotriene receptor 1 (CysLTR1) antagonist, on the contractility of the porcine uterine smooth muscle in the luteal phase of the oesterous cycle (n=8) and in early pregnancy (n=8). Stimulation of uterine strips in the luteal phase with MON has been shown to significantly reduce the amplitude of con- tractions, but not to affect the tension or frequency of contractions. A statistically significant tension increase and decrease in the frequency and amplitude of contractions was observed in pigs in early pregnancy. This suggests that MON has a different effect on the parameters under study in cyclic and pregnant pigs.
Early embryonic death (EED) is one of the causes of infertility in the mare. We compared endometrial environment in 9 mares with EED and 13 mares in diestrus phase. Cotton swab (CS), cytobrush (CB) and uterine biopsy (B) samples were obtained for the cytological, bacteriological and histopathological examinations. In the first step we compared CS and CB methods to biopsy as a reference method, as B revealed the highest number of positive results in cytological and bacteriological examinations in both groups. In turn, we also compared cytological, bacteri- ological and histopathological findings between EED and control animals using the B sampling. Although the differences between these groups were not statistically significant (p≥0.05), there was a tendency to a higher prevalence of subclinical endometritis in the control group, than in the EED group (62% vs 22%). In general, positive bacteriological results were similar in both groups (62% vs 55%), whereas positive cytological results were higher in the control group (62% vs 22%; p≥0.05). In histopathological examination in EED mares endometrial degeneration was better expressed (all mares were with grades IIB and III on the Kenney-Doig scale); however, the differences between both groups were not statistically significant (p≥0.05). We could not confirm a clear difference in uterine environment between the two groups. Moreover, the uterine biopsy seemed to be the most reasonable sampling method for diagnosis of endometrial state.
The chronic degenerative endometritis (CDE) is recognised as directly related to age and infertility in the mare. In this study, the 14 days post ovulation (PO) pregnancy rate was assessed in 60 barren mares affected by CDE, submitted to platelet-rich-plasma intrauterine infusion (PRPI) 24 hours PO. Data showed a significant positive effect of PRPI on the chance to become pregnant in mares affected by Kenney I-II CDE. The overall 14 days PO pregnancy rate was 75%. Sixty-nine % of the overall pregnancies was achieved with the 1st PRPI, but an additional 31% was obtained with the 2nd PRPI at the subsequent heat, increasing significantly the number of pregnancies. Although the 1st PRPI lead to a higher pregnancy rate than the 2nd PRPI (52 vs 23%), the repetition of the treatment had a significant positive effect on the overall pregnancies. Mare’s young age positively influenced the chance to become pregnant and was associated to endometritis Kenney I. Mares with endometritis Kenney I had better chance to become pregnant than those with endometritis Kenney II. The PRPI proved, therefore, to be beneficial in barren mares with CDE, and resulted in high overall pregnancy rate achievement in barren mares.