Search for: [Keywords = "secondary metabolites"]

Plant secondary metabolites as defenses: A review

Herlina Jusuf Marischa Elveny Feruza Azizova Rustem A. Shichiyakh Dmitriy Kulikov Muataz M. Al-Taee Karrar K. Atiyah Abduladheem T. Jalil Surendar Aravindhan

Journal of Water and Land Development | 2022 | No 55 | 206-211 | DOI: 10.24425/jwld.2022.142323

Keywords alkaloids flavonoids phenolics plant pathogens secondary metabolites

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Abstract

Plant secondary metabolites have a variety of functions, including mediating relationships between organisms, responding to environmental challenges, and protecting plants against infections, pests, and herbivores. In a similar way, through controlling plant metabolism, plant microbiomes take part in many of the aforementioned processes indirectly or directly. Researchers have discovered that plants may affect their microbiome by secreting a variety of metabolites, and that the microbiome could likewise affect the metabolome of the host plant. Pesticides are agrochemicals that are employed to safeguard humans and plants from numerous illnesses in urban green zones, public health initiatives, and agricultural fields. The careless use of chemical pesticides is destroying our ecology. As a result, it is necessary to investigate environmentally benign alternatives to pathogen management, such as plant-based metabolites. According to literature, plant metabolites have been shown to have the ability to battle plant pathogens. Phenolics, flavonoids, and alkaloids are a few of the secondary metabolites of plants that have been covered in this study.

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Authors and Affiliations

Herlina Jusuf

1

e-mail:

ORCID:

Marischa Elveny

2

e-mail:

ORCID:

Feruza Azizova

3

e-mail:

ORCID:

Rustem A. Shichiyakh

4

e-mail:

ORCID:

Dmitriy Kulikov

5

e-mail:

ORCID:

Muataz M. Al-Taee

6

e-mail:

ORCID:

Karrar K. Atiyah

7

e-mail:

ORCID:

Abduladheem T. Jalil

8

e-mail:

ORCID:

Surendar Aravindhan

9

e-mail:

ORCID:

Universitas Negeri Gorontalo, Faculty of Sports and Health, Department of Public Health, Jln. Jenderal Sudirman 6, Gorontalo, 96128, Indonesia
Universitas Sumatera Utara, DS & CI Research Group, Medan, Indonesia
Tashkent Medical Academy, Tashkent, Uzbekistan
Kuban State Agrarian University named after I.T. Trubilin, Department of Management, Kuban, Russia
Moscow State University of Technologies and Management named after K.G. Razumovsky (First Cossack University), Department of Digital Nutrition, Hotel and Restaurant Services, Moscow, Russia
AL-Nisour University College, Department of Medical Laboratories Technology, Baghdad, Iraq
College of Dentistry, Al-Ayen University, Thi-Qar, Iraq
Al-Mustaqbal University College, Medical Laboratories Techniques Department, Babylon, Hilla, Iraq
Saveetha Institute of Medical and Technical Sciences, Chennai, India

Antifungal activity of bioactive compounds produced by the endophyte Bacillus velezensis NC318 against the soil borne pathogen Sclerotium rolfsii Sacc

Michelle Gaëlle Siméone Bidima Noureddine Chtaina Brahim Ezzahiri Mohammed El Guilli Ilham Barakat Taha El Kamli

Journal of Plant Protection Research | 2022 | vol. 62 | No 4 | 326-333 | DOI: 10.24425/jppr.2022.142139

Keywords antifungal activity biological control sclerotia secondary metabolites Bacillus velezensis

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Abstract

In a previous study, the endophytic Bacillus velezensis NC318 was isolated from the rhizosphere of date palm and showed strong antifungal activity against the soil-borne plant pathogenic fungus, Sclerotium rolfsii Sacc, the causal agent of Southern blight. The potential of the Bacillus genus in the inhibition of plant pathogens is mainly due to the production of certain bioactive compounds. In the present study, secondary metabolites extracted from the cell-free supernatant of strain NC318 showed strong antifungal activity on the mycelial growth and germination of S. rolfsii sclerotia in vitro. With 50 μl of bioactive compounds crude extracts, the mycelial growth inhibition rate was 97% and any germination of sclerotia was reported. Chemical analysis of the secondary metabolite crude extracts performed by high performance liquid chromatography coupled with mass spectrometry (HPLC/MS), revealed that the secreted bioactive compounds belonged to the family of lipopeptides (iturin, fengycin, surfactin), polyketides (bacillaene, macrolactin, difficidin and bacilysin) and siderophores (bacillibactin). These results provide a better understanding of the biocontrol mechanism of the bacteria strain B. velezensis NC318 against the soil fungal pathogens, especially S. rolfsii root rot.

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Authors and Affiliations

Michelle Gaëlle Siméone Bidima

1

Noureddine Chtaina

1

Brahim Ezzahiri

1

Mohammed El Guilli

2

Ilham Barakat

1

e-mail:

ORCID:

Taha El Kamli

3

Plant Protection Unit, Hassan II Agronomic and Veterinary Institute, Rabat, Morocco
Plant Protection Unit, National Institute of Agronomic Research, Rabat, Morocco
Anti-Doping Control Laboratory, Hassan II Agronomic and Veterinary Institute, Kenitra, Morocco

Fall armyworm ( Spodoptera frugiperda) (J. E. Smith) (Lepidoptera: Noctuidae) infestation: maize yield depression and physiological basis of tolerance

Folake Bosede Anjorin Oluwaseyi Oluwakemi Odeyemi Olufolake Adenike Akinbode Kehinde Tolulope Kareem

Journal of Plant Protection Research | 2022 | vol. 62 | No 1 | 12-21 | DOI: 10.24425/jppr.2022.140294

Keywords allelochemicals crop yield herbivory plant defense secondary metabolites

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Abstract

Fall armyworm ( Spodoptera frugiperda) (FAW) is an important invasive pest of maize. The young FAW larva disrupts the photosynthetic system by feeding on the leaves. The older caterpillar interferes with pollination and fertilization processes, destroying the tassel and silks, or it bores into the maize cob, reducing harvest quality and predisposing the cob to secondary infections. The infested plant responds by channeling or converting the primary metabolites into secondary metabolites for plant defense, further reducing crop yield. The devastating feeding effect on maize becomes even more severe when maize plants are exposed to prolonged drought, during which the production of secondary metabolites is optimum. These secondary metabolites are food for herbivorous insects like the fall armyworm. Naturally, plants possess several adaptive features which enable them to cope and survive herbivorous insect attacks without compensating yield for plant defense. Such features include: thickening of the leaf cuticle of the epidermal cell walls, production of certain allelochemicals, defense proteins and the toxic chemical compound, favone glycoside (silk maysin). This review attempts to critically appraise the physiological implications of fall armyworm damage on developmental processes and maize yield. Understanding the mechanisms of various adaptive traits that confer resistance to maize against herbivorous insect damage would assist greatly in crop improvement processes.

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Authors and Affiliations

Folake Bosede Anjorin

1

Oluwaseyi Oluwakemi Odeyemi

1

Olufolake Adenike Akinbode

1

Kehinde Tolulope Kareem

1

Institute of Agricultural Research and Training, Obafemi Awolowo University, Ibadan, Nigeria

Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense

Jéssica Emília Rabuske Marlove Fátima Brião Muniz Thiarles Brun Mateus Alves Saldanha Janaina Silva Sarzi Lucas Gracioli Savian Clair Walker Jéssica Mengue Rolim Giovani Leone Zabot Marcio Antonio Mazutti

Journal of Plant Protection Research | 2023 | vol. 63 | No 4 | 488-498 | DOI: 10.24425/jppr.2023.147832

Keywords Carya illinoinensis chromatographic analysis culture filtrates secondary metabolites

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Abstract

Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense are pathogens causing trunk canker in Carya illioniensis and there are still no reports of effective forms of control. However, biological control is a promising measure. The objective of this work was to isolate, identify and evaluate the action of Trichoderma spp. in the in vitro control of L. theobromae and P. kimberleyense and to identify the compounds produced by the antagonist. Trichoderma spp. was identified by molecular technique and morphologically characterized. The antagonistic action of T. asperellum isolates (obtained from the rhizospheric soil, and of an isolate obtained from a commercial formulation) was evaluated by pairing cultures and volatile metabolites on L. theobromae and P. kimberleyense. Optimization of the cultivation method of T. asperellum was carried out and the compounds produced by the antagonist were identified by gas chromatography. Isolates obtained from the soil were identified as T. asperellum and decreased mycelial growth of L. theobromae and P. kimberleyense in the crop pairing test (48.98% S6 x Qt), as well as by volatile metabolites (29.85% SM21 x TR4). The cultivation conditions that generated the filtrates with the greatest antifungal action used 20 g · l ^–1 of corn maceration water, yeast extract 7.5 g · l ^–1, pH 5, agitation 100 rpm, sucrose 50 g · l ^–1, inoculum concentration 105 spores /ml. Among the identified compounds, some stood out for having bioactive action, such as pyran derivatives, celidoniol, deoxy, pentadecanoicacid, 2,3-dihydro-3,5-dihydroxy-6-methyl, propanoicacid, 1-methylethyl ester and 9-octadecenoic acid. The T. asperellum isolates showed potential for biocontrol in vitro, acting by different mechanisms.

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Authors and Affiliations

Jéssica Emília Rabuske

1

e-mail:

ORCID:

Marlove Fátima Brião Muniz

2

e-mail:

ORCID:

Thiarles Brun

1

e-mail:

ORCID:

Mateus Alves Saldanha

2

e-mail:

ORCID:

Janaina Silva Sarzi

2

e-mail:

ORCID:

Lucas Gracioli Savian

2

e-mail:

ORCID:

Clair Walker

2

e-mail:

ORCID:

Jéssica Mengue Rolim

3

e-mail:

ORCID:

Giovani Leone Zabot

4

e-mail:

ORCID:

Marcio Antonio Mazutti

5

e-mail:

ORCID:

Universidade Regional Integrada do Alto Uruguai e das Missões, Frederico Westphalen, Rio Grande do Sul, Brazil
Departamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Departamento de Fitotecnia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil
Laboratório de Processos de Engenharia Agroindustrial, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Departamento de Engenharia Química, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

Bioinformatic insight into Portulaca oleracea L. (Purslane) of Bulgarian and Greek origin

Vessela Balabanova Iassen Hristov Dimitrina Zheleva-Dimitrova Paulina Sugareva Valentin Lozanov Reneta Gevrenova

Acta Biologica Cracoviensia s. Botanica | 2020 | vol. 62 | No 1 | 7-21 | DOI: 10.24425/abcsb.2020.131662

Keywords Portulaca oleracea LC–HRAMS secondary metabolites descriptive analysis multivariate statistics

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Abstract

Portulaca oleracea L. (Portulacaceae) is used as functional food and its nutritional and therapeutic properties are related to the high levels of organic and fatty acids, polyphenols, polysaccharides and cyclo-dopa amides. This study presents a strategy based on liquid chromatography – high resolution accurate mass spectrometry method (LC – HRAMS) and bioinformatic methods to analyze 33 purslane accessions originating from 11 floristic regions in Bulgaria together with 5 accessions of Greek provenance. Extracts were obtained by microwave extraction. Based on the LC-MS metabolic “fingerprints” of assayed samples, a purslane metabolic database was developed. LC-MS data were proceeded with Software application Compound Discover 2.0 (Thermo Fischer Sci., USA). Principal Component Analysis (PCA) combined with both descriptive and differential analyses were used to find marker metabolites to distinguish different geographical regions. The differential analysis of the Bulgarian and Greek samples allowed the identification of 50 marker metabolites. Based on accurate masses, retention times, fragmentation patterns in MS/MS, comparison with commercial standards and literature data, these secondary metabolites were identified after detailed analysis of Volcano-plots. For the first time, 29 compounds are reported. The identified compounds were used to perform a study of the biosynthetic pathways of purslane secondary metabolites using Kyoto Encyclopedia of Genes and Genomes (KEGG) software platform. The statistical treatments identified marker compounds that can be used to distinguish the origin of accession set. Combining LC-MS data with multivariate statistical analysis was shown to be effective in studying the purslane metabolites, allowing for integration of chemistry with geographic origin.

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Authors and Affiliations

Vessela Balabanova

Iassen Hristov

Dimitrina Zheleva-Dimitrova

Paulina Sugareva

Valentin Lozanov

Reneta Gevrenova

Bioreactor study of the metabolic repertoire and morphology of actinomycete Streptomyces rimosus ATCC 10970 under various initial concentrations of carbon and nitrogen sources

Marcin Bizukojć Anna Ścigaczewska Tomasz Boruta Agnieszka Ruda Aleksandra Kawka

Chemical and Process Engineering: New Frontiers | Accepted articles | e56 | DOI: 10.24425/cpe.2023.147414

Keywords actinomycetes batch bioreactor secondary metabolites Streptomyces rimosus microbial morphology

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Abstract

This work aims at investigating the influence of the initial concentrations of carbon (glucose) and organic nitrogen (yeast extract) sources on Streptomyces rimosus ATCC10970 secondary metabolism in the stirred tank bioreactors. Additionally, glucose utilisation, biomass formation, pH, redox potential and dissolved oxygen levels, and the morphological development of S. rimosus pseudomycelium were studied. Eighteen secondary metabolites were detected by mass spectrometry and identified with the use of the authentic standard, or putatively with the use of literature and database of secondary metabolites. Varied initial yeast extract concentration acted much stronger on the formation of secondary metabolites than glucose did. For example, oxytetracycline was not biosynthesised at high yeast extract concentration while the formation of three other metabolites was enhanced under these conditions. In the case of glucose its increasing initial concentration led to higher secondary metabolite levels with the exception of an unnamed angucycline. High initial yeast extract concentration also drastically changed S. rimosus pseudomycelial morphology from the pelleted to the dispersed one. Ultimately, the cultivation media with the varied initial levels of carbon and nitrogen sources were proved to have the marked effect on S. rimosus secondary metabolism and to be the simplest way to either induce or block the formation of the selected secondary metabolites.

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Authors and Affiliations

Marcin Bizukojć

1

e-mail:

ORCID:

Anna Ścigaczewska

1

e-mail:

ORCID:

Tomasz Boruta

1

e-mail:

ORCID:

Agnieszka Ruda

1

Aleksandra Kawka

1

Lodz University of Technology, Faculty of Process and Environmental Engineering,Department of Bioprocess Engineering, Wólczańska 213, 93-005 Łódź, Poland

Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites

Yoke−Kqueen Cheah Learn−Han Lee Cheng−Yun Catherine Chieng Vui−Ling Clemente Michael Wong

Polish Polar Research | 2015 | No 1 | 67-78 | DOI: 10.1515/popore−2015−0001

Keywords Antarctic Actinobacteria secondary metabolites 16S diffusion assay selective isolation media

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Abstract

This project aimed to isolate and characterize volcanic soil Actinobacteria from Deception Island, Antarctic. A total of twenty−four Actinobacteria strains were isolated using four different isolation media (Starch casein agar, R2 agar, Actinomycete isolation agar, Streptomyces agar) and characterized basing on 16S rRNA gene sequences. Tests for secondary metabolites were performed using well diffusion method to detect antimicrobial activities against eight different pathogens, namely Staphyloccocus aureus ATCC 33591, Bacillus megaterium , Enterobacter cloacae , Klebsiella oxytoca , S. enterica serotype Enteritidis, S. enterica serotype Paratyphi ATCC 9150, S. enterica serotype Typhimurium ATCC 14028 and Vibrio cholerae . Antimicrobial properties were detected against Salmonella paratyphi A and Salmonella typhimurium at the concentration of 0.3092±0.08 g/ml. The bioactive strains were identified as Gordonia terrae , Leifsonia soli and Terrabacter lapilli. Results from this study showed that the soil of Deception Island is likely a good source of isolation for Actinobacteria. The volcanic soil Actinobacteria are potentially rich source for discovery of antimicrobial compounds.

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Authors and Affiliations

Yoke−Kqueen Cheah

Learn−Han Lee

Cheng−Yun Catherine Chieng

Vui−Ling Clemente Michael Wong

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Plant secondary metabolites as defenses: A review

Abstract

Authors and Affiliations

Antifungal activity of bioactive compounds produced by the endophyte Bacillus velezensis NC318 against the soil borne pathogen Sclerotium rolfsii Sacc

Abstract

Authors and Affiliations

Fall armyworm ( Spodoptera frugiperda) (J. E. Smith) (Lepidoptera: Noctuidae) infestation: maize yield depression and physiological basis of tolerance

Abstract

Authors and Affiliations

Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense

Abstract

Authors and Affiliations

Bioinformatic insight into Portulaca oleracea L. (Purslane) of Bulgarian and Greek origin

Abstract

Authors and Affiliations

Bioreactor study of the metabolic repertoire and morphology of actinomycete Streptomyces rimosus ATCC 10970 under various initial concentrations of carbon and nitrogen sources

Abstract

Authors and Affiliations

Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites

Abstract

Authors and Affiliations