@ARTICLE{Youssef_A.I._PCR-based_2021, author={Youssef, A.I. and Afifi, A. and Abbadi, S. and Hamed, A. and Enany, M.}, volume={vol. 24}, number={No 3}, journal={Polish Journal of Veterinary Sciences}, pages={445-450}, howpublished={online}, year={2021}, publisher={Polish Academy of Sciences Committee of Veterinary Sciences}, publisher={University of Warmia and Mazury in Olsztyn}, abstract={Helicobacter species have been reported in animals, some of which are of zoonotic importance. This study aimed to detect Helicobacter species among human and animal samples using conventional PCR assays and to identify their zoonotic potentials. Helicobacter species was identified in human and animal samples by genus-specific PCR assays and phylogenetic analysis of partial sequencing of the 16S ribosomal RNA gene. The results revealed that Helicobacter species DNA was detected in 13 of 29 (44.83%) of the human samples. H. pylori was identified in 2 (15.38%), and H. bovis was detected in 4 (30.77%), whereas 7 (53.85%) were unidentified. H. bovis and H. heilmannii were prevalent among the animal samples. Phylogenetic analysis revealed bootstrapping of sequences with H. cinaedi in camel, H. rappini in sheep and humans, and Wollinella succinogenes in humans. In conclusion, the occurrence of non-H. pylori infections among human and animal samples suggested zoonotic potentials.}, type={Article}, title={PCR-based detection of Helicobacter pylori and non-Helicobacter pylori species among humans and animals with potential for zoonotic infections}, URL={http://journals.pan.pl/Content/120939/16_Youssef.pdf}, doi={10.24425/pjvs.2021.138737}, keywords={Helicobacter spp., zoonotic infections, detection, identification}, }