@ARTICLE{Knapik-Czajka_Małgorzata_Influence_2021,
 author={Knapik-Czajka, Małgorzata and Bieleń, Justyna and Zajonz, Monika and Gawędzka, Anna and Drąg, Jagoda and Belczyk, Małgorzata},
 volume={Vol. 61},
 number={No 3},
 journal={Folia Medica Cracoviensia},
 pages={55-63},
 howpublished={online},
 year={2021},
 publisher={Oddział PAN w Krakowie; Uniwersytet Jagielloński – Collegium Medicum},
 abstract={White adipose tissue plays an important role in the catabolism of branched chain amino acids (BCAAs). Two initial regulatory steps in BCAAs catabolism are catalyzed by branched chain aminotrans-ferase (BCAT) and branched chain α-keto acid dehydrogenase complex (BCKDH complex), respectively. It has been demonstrated that synthetic ligands for PPARγ receptors increased mRNA levels for enzymes involved in BCAAs catabolism. We hypothesized that feeding rats with diet rich in linoleic acid (LA), a natural PPARγ agonist modifies mRNA levels for enzymes catalyzing BCAAs degradation in adipose tissue. The current pilot study was aimed at the investigation of the effect of diet rich in LA on mRNA levels for BCATm, branched chain α-keto acid dehydrogenase (E1 component of the BCKDH), and mRNA levels for the regulatory enzymes of BCKDH complex, a specific kinase (BDK) and a specific phosphatase (PPM1K) in epididymal white adipose tissue (eWAT). Wistar male rats were fed with high unsaturated fat diet containing mainly linoleic acid (study group) or with the high saturated fat diet (control group). The relative mRNA levels were quantified by reverse transcription PCR. We have found that in rats fed diet rich in LA mRNA level for BCATm decreased, while mRNA amount for BDK increased. There was no difference between mRNA levels for BCKDH E1 and PPM1K. It is con-ceivable that changes in mRNA levels for enzymes involved in BCAAs metabolism in eWAT may lead to modification of BCAAs catabolic rate. Further studies are required to fully elucidate this issue.},
 type={Article},
 title={Influence of a diet rich in linoleic acid on mRNA levels for enzymes of branched chain amino acids metabolism in rat’s adipose tissue: a pilot study},
 URL={http://journals.pan.pl/Content/122084/PDF-MASTER/2021-03-FMC-04-Knapik.pdf},
 doi={10.24425/fmc.2021.138951},
 keywords={branched chain amino acids (BCAAs), branched chain α-ketoacid dehydrogenase (BCKDH), linoleic acid, adipose tissue, rat},
}