@ARTICLE{Widjiati_W._Calcium_2022,
 author={Widjiati, W. and Faizah, Z. and Darsini, N. and Hendrawan, V.F. and Karima, H.N. and Chotimah, C. and Sumitro, S.B. and Yustinasari, L.R. and Kasman, A.A.M.N. and Ntoruru, J.M. and Luqman, E.M.},
 volume={vol. 25},
 number={No 1},
 journal={Polish Journal of Veterinary Sciences},
 pages={19-26},
 howpublished={online},
 year={2022},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.},
 type={Article},
 title={Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs) in Kacang goat after vitrification},
 URL={http://journals.pan.pl/Content/122653/PDF-MASTER/3%20_%20Widjiati.pdf},
 doi={10.24425/pjvs.2022.140836},
 keywords={Calcium (Ca2+), confocal laser scanning microscope, cumulus-oocyte complex (COCs), food production, Kacang goat},
}