@ARTICLE{Zhang_Y._Protective_2022,
 author={Zhang, Y. and Xu, W. and Tang, Y. and Huang, F.},
 volume={vol. 25},
 number={No 1},
 journal={Polish Journal of Veterinary Sciences},
 pages={35-44},
 howpublished={online},
 year={2022},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={Porcine contagious pleuropneumonia (PCP) is a very serious respiratory disease which is difficult to prevent and treat. In this study, the therapeutic effects of lithium chloride (LiCl) on PCP were examined using a mouse model. A mouse model of PCP was established by intranasal infections with Actinobacillus pleuropneumoniae (App). Histopathological analysis was performed by routine paraffin sections and an H-E staining method. The inflammatory factors, TLR4 and CCL2 were analyzed by qPCR. The expression levels of p-p65 and pGSK-3ß were detected using the Western Blot Method. The death rates, clinical symptoms, lung injuries, and levels of TLR-4, IL-1ß, IL-6, TNF-α, and CCL2 were observed to decrease in the App-infected mice treated with LiCl. It was determined that the LiCl treatments had significantly reduced the mortality of the App-infected cells, as well as the expressions of p-p65 and pGSK-3ß. The results of this study indicated that LiCl could improve the pulmonary injuries of mice caused by App via the inhibition of the GSK-3β-NF-κB-dependent pathways, and may potentially become an effective drug for improving pulmonary injuries caused by PCP.},
 type={Article},
 title={Protective effect of lithium chloride on pulmonary injury caused by Actinobacillus pleuropneumoniae via inhibition of GSK-3β-NF-κB-dependent pathway},
 URL={http://journals.pan.pl/Content/122655/PDF/5%20_%20Zhang.pdf},
 doi={10.24425/pjvs.2022.140838},
 keywords={porcine contagious pleuropneumonia, mouse model, lithium chloride, toll-likereceptor 4, GSK-3β-NF-κB-dependent pathways},
}