@ARTICLE{Stefanek_Agata_Biomimetic_2022,
 author={Stefanek, Agata and Kulikowska-Darłak, Aleksandra and Bogaj, Karolina and Nowak, Aleksandra and Dembska, Joanna and Ciach, Tomasz},
 volume={vol. 43},
 number={No 1},
 journal={Chemical and Process Engineering},
 pages={81-95},
 howpublished={online},
 year={2022},
 publisher={Polish Academy of Sciences Committee of Chemical and Process Engineering},
 abstract={Cell encapsulation seems to be a promising tool in tissue engineering. However, it has been shown to have several limitations in terms of long-term cell cultures due to an insufficient oxygen supply. In this study we propose the use of novel microcapsules designed for long-term cell culture consisting of an alginate shell and perfluorocarbon (PFC) core, which works as a synthetic oxygen carrier and reservoir. The influence of PFC presence in the culture as well as the size of structures on cell metabolism was evaluated during 21-day cultures in normoxia and hypoxia. We showed significant improvement in cell metabolism in groups where cells were encapsulated in hydrogel structures with a PFC core. The cells maintained a typical metabolism (oxidative phosphorylation) through all 21 days of the culture, overcoming the oxygen supply shortage even in large structures (diameter ¡ 1 mm). Applying PFC in alginate matrices can improve cell metabolism and adaptation in long-term cell cultures.},
 type={Article},
 title={Biomimetic alginate/perfluorocarbon microcapsules – the effect on in vitro metabolic activity and long-term cell culture},
 URL={http://journals.pan.pl/Content/123265/PDF-MASTER/art05_internet.pdf},
 doi={10.24425/cpe.2022.140812},
 keywords={alginate, microcapsules, cell culture, perfluorocarbons, metabolism},
}