@ARTICLE{Mao_Lutian_Composition_2022,
 author={Mao, Lutian and Chen, Lifen and Wang, Xirui and Xu, Zhongbao and Ouyang, Hui and Huang, Biyou and Zhou, Libin},
 volume={vol. 48},
 number={No 4},
 pages={95-101},
 journal={Archives of Environmental Protection},
 howpublished={online},
 year={2022},
 publisher={Polish Academy of Sciences},
 abstract={To analyze the composition of norfloxacin-resistant bacteria and norfloxacin-degrading bacteria in pond water and sediment in subtropical China, the composition of antibiotic resistant bacteria in pond water and sediment enriched with norfloxacin-containing medium was analyzed by high-throughput sequencing. Sediment and water samples were collected from 3 fish ponds in subtropical China, and domesticated with norfloxacin, subsequently norfloxacin-resistant bacteria through high-throughput sequencing of 16S rDNA, and isolated norfloxacin- degrading bacteria. Our results showed that the pond sediment and water contain a variety of norfloxacin-resistant bacteria, mainly from Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Chloroflexi. Moreover, we isolated two norfloxacin-degrading bacteria (NorXu-2 and NorXu-3). The norfloxacin-degrading rate by NorXu-2 and NorXu-3 in the culture mediums with 200 μg/mL was the highest, which was up to 49.71% and 35.79%,respectively. When the norfloxacin concentration was 200 μg/mL, NorXu-2 and NorXu-3 had the best norfloxacin-degrading effect at pH of 6, and the degradation rates were 53.64% and 45.54%, respectively. Moreover, NorXu-3 exhibited a good tolerance to high NaCl concentration. These results not only provided basic data for the follow-up study of the molecular mechanism of antimicrobial microbial degradation, but also provided potential norfloxacin degrading bacteria for norfloxacin removal and bioremediation in aquaculture environment.},
 type={Article},
 title={Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China},
 URL={http://journals.pan.pl/Content/125246/PDF/Archives%20vol48no4pp94_101.pdf},
 doi={10.24425/aep.2022.143712},
 keywords={high-throughput sequencing, norfloxacin, antibiotic resistant bacterium, pond culture},
}